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Method for in-situ synthesis of nano magnetite by soil microorganisms

A technology of soil microorganisms and nano-magnets, which is applied in the field of in-situ synthesis of nano-magnetites by soil microorganisms, can solve the problems of increasing repair costs and difficult electronic mediators, and achieve the effect of improving mass transfer capacity

Pending Publication Date: 2021-03-02
NANKAI UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

However, in soil, magnetite and other conductive minerals are often surrounded by organic matter or pollutants, making it difficult to function as an electron mediator, and adding magnetite from an external source will increase the repair cost

Method used

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  • Method for in-situ synthesis of nano magnetite by soil microorganisms
  • Method for in-situ synthesis of nano magnetite by soil microorganisms
  • Method for in-situ synthesis of nano magnetite by soil microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] 1. Experimental group

[0028] 1) Enrichment of soil microorganisms

[0029] Grind and sieve the harvested farmland soil and weigh 1000g to the acrylic conical leach reactor with a bottom diameter of 10cm and a height of 15cm. Extraction liquid collector, use 5mL / h water flow into the collector, stir the soil deposition liquid with a glass rod every certain time, the whole process is repeated 2-3 times to complete the extraction of soil extraction liquid, the device diagram is as follows figure 1 shown;

[0030] Add 4g / L sodium acetate and 1g / L ferric citrate to the extracted soil extract, carry out anaerobic sealing treatment, and carry out the cultivation of soil microorganisms and the directional enrichment of microorganisms that can reduce iron at a constant temperature of 30°C. Set, extract the solution regularly, measure the optical density value in it, and determine the microbial enrichment effect.

[0031] 2) Microbial synthesis of nano-magnetite

[0032] Th...

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Abstract

The invention discloses a method for in-situ synthesis of nano magnetite by soil microorganisms. Aiming at the problems of poor soil mass transfer capacity, the method mainly comprises the following steps: enrichment of soil microorganisms, microbial synthesis of nano magnetite and measurement of the nano magnetite. The enrichment of the soil microorganisms is mainly completed through the steps ofsoil leaching solution extraction, microorganism directional enrichment and the like. The microbial synthesis of the nano magnetite is mainly completed through the steps of precursor preparation, microorganism electron donor addition and the like. The measurement of the nano magnetite is mainly completed by measuring the content of iron ions in the nano magnetite through hydrochloric acid digestion, and performing infrared spectroscopy, X-ray diffraction pattern and transmission electron microscope. The method is simple to operate, the nano magnetite can be formed in situ in the soil and is uniformly distributed, and a new thought is provided for remediation of the contaminated soil.

Description

technical field [0001] The invention belongs to the field of environmental microbial geochemistry, in particular to a method for synthesizing nanometer magnetite in situ by soil microorganisms. Background technique [0002] With the acceleration of industrialization, environmental pollution has become an important factor restricting economic development and a serious threat to human survival and development. Heavy metal and organic pollution are two common types of pollution in soil and water environment. Microbes complete the recovery of heavy metals and the degradation of organic matter through redox processes in these two media. However, compared with the water environment, the mass transfer capacity of the soil environment itself Poor, the oxidation-reduction process of microorganisms is greatly hindered, greatly reducing the removal efficiency of pollutants. [0003] Iron (Fe) is an important highly active and abundant redox element in soil, and its biogeochemical cycl...

Claims

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Application Information

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IPC IPC(8): C12P3/00G01N21/3563G01N23/04G01N23/20G01N23/207
CPCC12P3/00G01N21/3563G01N23/04G01N23/20G01N23/207G01N2021/3572
Inventor 李田王鑫田丽丽
Owner NANKAI UNIV
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