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Enzymatic extraction process of fatty acid

A technology for enzymatic extraction of fatty acids, applied in the field of enzymatic extraction of fatty acids, can solve the problems of low yield of fatty acids, instability of gossypol, etc., and achieve the effect of increasing the yield of fatty acids, increasing the yield, and mild reaction

Pending Publication Date: 2021-03-05
无锡优普克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the alkali refining process, excess alkali is added, so that part of the neutral oil is also saponified and transferred into the soapstock; at the same time, part of the neutral oil is inevitably entrained in the soapstock, in addition to alkali, glycerin, protein, pigment etc.; the gossypol in the cotton oil is also brought in the soapstock, and the gossypol is very unstable in an alkaline medium, and is quickly converted into various dark compounds that make the soapstock have dark brown to black. The yield of fatty acid extracted from feet or oily feet is low

Method used

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  • Enzymatic extraction process of fatty acid

Examples

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Effect test

Embodiment 1

[0043] A kind of enzymatic extraction process of fatty acid, comprises the following steps:

[0044] 1) Add 300g of soapstock into the container, and add 600g of water and stir evenly to obtain the first mixture;

[0045] 2) Adjust the pH of the first mixture to 4 by using 10% acetic acid solution;

[0046] 3) Add 0.15g compound enzyme to the first mixture, the compound enzyme comprises 40wt% acid lipase, 40wt% neutral lipase and 20wt% phospholipase A2, the temperature is controlled at 40°C, stirred for 24h to carry out the enzymolysis reaction, to obtain the second mixture;

[0047] 4) The temperature of the second mixture after the enzymolysis reaction in step 3) was raised to 80° C., and kept for 30 minutes;

[0048] 5) Add 5% NaCl as a settling agent to the second mixture treated in step 4), and naturally lower the temperature and settle for 24 hours;

[0049] 6) The second mixture after sedimentation is subjected to oil-water separation to obtain fatty acid.

Embodiment 2

[0051] A kind of enzymatic extraction process of fatty acid, comprises the following steps:

[0052] 1) Add 400g of soapstock into the container, add 1200g of water and stir evenly to obtain the first mixture;

[0053] 2) Adjust the pH of the first mixture to 5 with 20% citric acid solution;

[0054]3) Add 0.4 g of compound enzyme to the first mixture, the compound enzyme includes 45wt% acid lipase, 36wt% neutral lipase and 19wt% phospholipase A2, control the temperature at 50°C, stir for 50h to carry out enzymolysis reaction, and obtain the second Two mixtures;

[0055] 4) The temperature of the second mixture after the enzymatic hydrolysis reaction in step 3) was raised to 85° C., and kept for 40 minutes;

[0056] 5) Add 10% NaCl as a settling agent to the second mixture treated in step 4), and naturally cool down and settle for 48 hours;

[0057] 6) The second mixture after sedimentation is subjected to oil-water separation to obtain fatty acid.

Embodiment 3

[0059] A kind of enzymatic extraction process of fatty acid, comprises the following steps:

[0060] 1) Add 500g of oil base into the container, and add 2500g of water and stir evenly to obtain the first mixture;

[0061] 2) Adjusting the pH of the first mixture to 6 with 20% citric acid solution;

[0062] 3) Add 2.5 g of compound enzyme to the first mixture, the compound enzyme includes 50wt% acid lipase, 40wt% neutral lipase and 10wt% phospholipase A2, control the temperature at 60°C, stir for 72h to carry out enzymolysis reaction, and obtain the second Two mixtures;

[0063] 4) heating the second mixture after the enzymatic hydrolysis reaction in step 3) to 90° C., and keeping it warm for 60 minutes;

[0064] 5) Add 20% NaCl as a settling agent to the second mixture treated in step 4), and naturally lower the temperature and settle for 72 hours;

[0065] 6) The second mixture after sedimentation is subjected to oil-water separation to obtain fatty acid.

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Abstract

The invention discloses an enzymatic extraction process of fatty acid. The process comprises the following steps: 1) adding soapstock or oil residue into a container, adding water, and uniformly stirring to obtain a first mixture; 2) adjusting the pH value of the first mixture to 4-6 by adopting an acid solution; 3) adding a compound enzyme into the first mixture, controlling the temperature, andstirring for 24-72 hours to perform enzymolysis reaction to obtain a second mixture; 4) heating the second mixture subjected to the enzymolysis reaction in the step 3) to 80-90 DEG C, and keeping thetemperature for a certain time; 5) adding a settling agent into the second mixture treated in the step 4), and performing natural cooling and settling; and 6) carrying out oil-water separation on thesettled second mixture to obtain the fatty acid. According to the enzymatic extraction process of the fatty acid, the compound enzyme is added into the soapstock or the oil residue for enzymolysis reaction, the compound enzyme preparation can be used for accelerating the reaction, the reaction is mild, and the yields of neutral oil and the fatty acid are increased.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to an enzymatic extraction process of fatty acids. Background technique [0002] Chinese people mainly eat vegetable oil, and most edible oils need to be refined. Depending on the type of crude oil, the impurity content, and the grade of edible oil, their refining does not matter, but they are generally divided into two categories: alkali refining and hydration. The precipitate obtained from alkali refining is called soapstock, and the precipitate obtained from hydration is called soapstock. It is called oil foot, and the difference is that the former uses liquid alkali to neutralize the free fatty acid in the crude oil into fatty acid sodium salt and forms a soap colloid, which contains impurities such as neutral oil and pigment, and the latter uses water to neutralize the free fatty acid in the crude oil. The phospholipid absorbs water and swells to form a colloid, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B13/02C11B13/00
CPCC11B13/02C11B13/00Y02W30/74
Inventor 周建根刘飞刘成林范军伟王媚艳
Owner 无锡优普克生物科技有限公司
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