Optical clearing agent and preparation method thereof

Abstract

The invention relates to the technical field of biological imaging, in particular to an optical clearing agent and a preparation method thereof. The optical clearing agent comprises a first componentand a second component, wherein the first component is composed of PEG400 and glycerol in the volume ratio of (0.2-5.0): 1; the second component is thiazone; and the volume ratio of the first component to the second component is (1-50): 1. The optical clearing agent obtained by selecting the specific components and compounding the components according to the specific ratio can reduce the scattering degree of tissues to light, and compared with other optical clearing agents in the prior art, the optical clearing agent provided by the invention has the advantage that the optical tissue penetration depth is larger.

Description

technical field [0001] The invention relates to the technical field of biological imaging, in particular to an optical transparent agent and a preparation method thereof. Background technique [0002] In recent years, with the development of optical devices, the medical application of light has become more and more mature, and has also spawned some new disciplines, such as biomedical photonics. At present, light has been widely used in the clinical diagnosis and treatment of diseases, including medical imaging (such as fluorescence imaging, up-conversion imaging, photothermal imaging, photoacoustic imaging, etc.), phototherapy (including photothermal therapy, photodynamic therapy, laser ablation, UV therapy, etc.) and optogenetics. In medical imaging, near-infrared light is used to excite indocyanine green (ICG) fluorescence imaging to observe its clearance in the human body in real time, so as to judge the liver reserve function of patients. In terms of photothermal thera...

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Problems solved by technology

However, there is still a certain difference between the refractive index of these light clearing agents and the essence of the tissue, which cannot achieve optimal light transparency of the tissue, and the refractive index of the tissue in different parts is also slightly different, and the refractive index of these light clearing agents reported It cannot be adjusted arbitrarily as needed to be suitable for the phototransparency of different tissue parenchyma, so there are still huge challenges in realizing the phototransparency of living tissues

[0004] Moreover, the photomedical applications of existing light-transparent agents are mainly limited to the fields of optical imaging or optical fluorescence imaging, and no light-transparent agents have been used in other photomedical fields such as phototherapy or optogenetics. For other emerging It is very necessary to enhance the depth of light penetration in the optical field to improve its effect, and it has great clinical significance and value

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