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Method for cultivating new transgenic insect-resistant glyphosate-resistant cotton strain

A technology of glyphosate resistance and cultivation method is applied in the field of cultivation of new lines of transgenic insect-resistant and glyphosate-resistant cotton, which can solve the problems of lack of research, and achieve the effects of increasing profits, speeding up replacement, and rapid popularization and application.

Inactive Publication Date: 2021-03-12
四川省农业科学院经济作物育种栽培研究所
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Problems solved by technology

[0003] Cotton transgenic technology has made significant progress in genetic improvement and has become a successful example of the application of biotechnology in crop genetic improvement. However, the transgenic cotton currently available can only guarantee one kind of resistance, such as insect resistance or glyphosate resistance. However, how to use non-transgenic means to integrate insect resistance and glyphosate resistance genes into plants to obtain a new multi-resistant cotton variety / line has not been studied in detail at present

Method used

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  • Method for cultivating new transgenic insect-resistant glyphosate-resistant cotton strain
  • Method for cultivating new transgenic insect-resistant glyphosate-resistant cotton strain
  • Method for cultivating new transgenic insect-resistant glyphosate-resistant cotton strain

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Embodiment 1

[0033] Breeding of New Transgenic Cotton Line RK635 with Insect-resistant and Glyphosate-resistant

[0034] (1) In the summer of 2015, the transgenic cotton variety Lumianyan 28 and the glyphosate-resistant line GGK-2 were planted in Chengdu, Sichuan. When Lumianyan No. 28 grows to 7 true leaves fully unfolded, the leaves that have just unfolded after being smeared with 1.0% karamycin sulfate solution with a mass percentage concentration, the leaves of all plants do not change color after 9 days, and Lumianyan 28 No. 100% insect-resistant, the insect-resistant gene is homozygous; GGK-2 grows to 5 true leaves when fully unfolded, its growth point is sprayed with a 0.2% glyphosate solution by mass percentage, after 10 days all cotton The growth of the seedlings was normal, and the growth point did not wilt or die, indicating that GGK-2 was 100% resistant to glyphosate, and the glyphosate-resistant gene was homozygous. After the flowering period, the stamens of the Lumianyan 28 ...

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Abstract

The invention provides a method for cultivating a new transgenic insect-resistant glyphosate-resistant cotton strain, and relates to the technical field of new crop variety breeding. The method comprises the following steps of hybridizing a transgenic cotton insect-resistant variety with a glyphosate-resistant strain, backcrossing by taking a filial generation as a female parent and the transgenicinsect-resistant variety as a male parent, continuously backcrossing for five generations by taking an insect-resistant glyphosate-resistant plant of a backcrossed generation as the female parent andthe transgenic insect-resistant variety as a recurrent parent, selecting the insect-resistant glyphosate-resistant plant for selfing for three generations, and screening insect-resistant and glyphosate-resistant plants for selfing to obtain the new transgenic insect-resistant and glyphosate-resistant cotton strain. The cultivation method is simple, practical and high in operability, the target traits can be aggregated in a short time, and the cultivated new variety retains the excellent traits of the original variety and can be rapidly popularized and applied, and variety updating is accelerated.

Description

technical field [0001] The invention belongs to the technical field of breeding of new crop varieties, and in particular relates to a method for cultivating new strains of transgenic insect-resistant and glyphosate-resistant cotton. Background technique [0002] Cotton is the largest economic crop in my country and occupies a very important position in the national economy. Cotton fiber is an important textile raw material, and cottonseed oil and cottonseed protein are important vegetable oil and protein resources. With the rapid development of our country's economy, the improvement of people's living standards, and the improvement of environmental protection awareness, the demand for pure cotton textiles is increasing. The production also requires multi-resistant cotton varieties to reduce environmental pollution caused by chemical pesticide control. . One of the most important ways to achieve the goals of reducing labor input, increasing yield, and reducing chemical pesti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 陈四维陈正杰牟方生钟文娟周永航戢沛城杨泽湖蒋理石盛佳宛永璐毛正轩王小强
Owner 四川省农业科学院经济作物育种栽培研究所
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