Bone tissue filling material containing levistilide A
A technology of angelica lactone and filling materials, which is applied in the field of biomedical engineering, can solve the problems of expensive fees, side effects, local inflammatory reactions and heterotopic ossification clinicians, and achieve low cost, easy preservation, production and get easy results
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Embodiment 1
[0022]Example 1 Preparation of EuDoncutum
[0023]Grind a certain amount of Chuanxion to the powder and placed in the flask. Anhydrous ethanol was added, and the ultrasound extracted at a certain condition (power: 150-200 W; frequency 40-60 kHz), and the chuanxiong extract was obtained.
[0024]The petroleum ether was added to the silica gel and added a column, evacuated, and drove the gas.
[0025]After taking the Sichuan extract, the chromatography was added to the chromatograph (2-6: 1) to elution at a certain gradient (2-6: 1) to obtain a light yellow oil extract.
Embodiment 2
[0026]Example 2 Preparation of a methacrylic hydrid material
[0027]a. Methacrylic modified gelatin preparation
[0028]4-7 g of gelatin was dissolved with 30-70 ml of phosphate buffered salts, placed in a round of flask. The solution was heated to 50-70 ° C and stirred by magnetic force for 0.5-2 hours, and a concentration of 8-12% gelatin solution was produced. Under the continuous stirring, 4-7 mL of methacrylate was added dropwise at 0.3-1 ml / min. After 1.5-3 hours of reaction, the reaction was terminated at 150-300 mL of a buffer solution preheated to 35-55 ° C. The dialysis bag with an intercepting molecular weight of 11-14 kDa was dialyzed under pure water conditions at 45-60 ° C for 6-9 days, and pure water was replaced every 4-8 hours. After dialysis is completed. Cool to room temperature and centrifuge at 6000-100,000 rpm for 10-30 minutes. After 1-3 times from extraordinary. The solution pH was adjusted to 7.0-7.4 and lyophilized for 70-90 hours. Finally, a white flocculatio...
Embodiment 3
[0033]Example 3 Preparation of bone tissue filler materials and different doses of EuDoncutum A promotion of bone tissue healing and testing of hematopoietic growth
[0034]The role of EuDoncutum-A promoting bone tissue and the growth of hemorrhate growth were investigated by mouse ectopic maneura model.
[0035]Use C57BL / 6, 6-9W-age and long and weighs about 25-40g; in accordance with the length of 0.5-2cm, width 0.2-0.4cm, high 0.4-0.7cm size Prepare the freeze-dried sponge sample of gel material, each freeze Sponge samples were lyophilized sponges treated with RHBMP-2 (unified additions 6-15μg).
[0036]Using DMSO to configure 15-35 mg / ml concentration of Eu Dudu-A solution, Eu Duddin Relidate -a is added to the previously lyophilized sample after the given dose gradient conversion amount, continues to freeze dry 10-15h Get the final sample; each set of samples is 2.
[0037]The amount of EuDoncutheryl in each group of samples is shown in Table (Experimental Mouse weight by 40G):
[0038]
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