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Equivalent circuit microfluidic concentration gradient chip with simplified channel

A technology of concentration gradient and equivalent circuit, which is applied in the direction of laboratory containers, chemical instruments and methods, laboratory utensils, etc., can solve the problems of lengthy channels, achieve the effects of promoting diffusion, easy processing, and reducing pressure drop

Pending Publication Date: 2021-03-12
NANCHANG HANGKONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a simplified and effective concentration gradient chip for the shortcoming of the existing equivalent circuit microfluidic concentration gradient chip.

Method used

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  • Equivalent circuit microfluidic concentration gradient chip with simplified channel
  • Equivalent circuit microfluidic concentration gradient chip with simplified channel
  • Equivalent circuit microfluidic concentration gradient chip with simplified channel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Simplify the design of the microchannel network formed by the concentration gradient of the equivalent circuit (take the double concentration gradient as an example)

[0025] Firstly, the equivalent circuit method is used to realize the double-digit concentration gradient chip designed in the traditional way to verify the effectiveness of the method, as shown in Figure 5 As shown, according to the principle of equivalent circuit design, the chip has two inlets, which are sample and diluent inlets respectively (the sample inlet flow rate is named as Q Sample , and the diluent inlet flow is named Q Buffer ), six outlets (named C in order of concentration from low to high 0 -C 5 ), the flow resistance of the liquid mixing channel is named R Mixing , the flow resistance of the connecting channel is named R connect . Known R Mixing =x,R connect = y, need to solve R 0 -R 8 The expression of the two-fold concentration gradient outlet concentration C 0 =0,C 1 =0.062...

Embodiment 2

[0056] Example 2: Comparison of concentration gradient formation before and after simplification and analysis of optimal conditions for stability

[0057] In order to verify the effectiveness of the designed equivalent circuit microfluidic network before and after the simplification, this embodiment took two-fold dilution as the target, and tested its effect of generating a two-fold concentration gradient. Such as Figure 4 As shown, the concentration ratios produced at the six outlets are 0, 0.0625, 0.125, 0.25, 0.5, 1 in turn. The microfluidic network adopts Embodiment 1 to design an unsimplified microfluidic network. According to the result obtained in the embodiment, the ratio of diluent and sample inlet flow is about 2, therefore, image 3 The ratio of twice the flow rate of multiple groups (120-60, 60-30, 30-15, 15-7.5μl / min) was selected. According to the calculation fluid dynamics simulation results, when the diluent and sample inlet flow rates are 60 When -30μl / min...

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Abstract

The invention discloses an equivalent circuit microfluidic concentration gradient chip with simplified channels. The chip comprises at least two liquid inlets used for being connected with a connecting channel, a flow resistance channel, a mixing channel and an outlet channel, and is characterized in that the mixing channel adopts a novel mixing channel with a transverse elliptical bulge structureto replace a traditional linear mixing channel; therefore, the mixing effect superior to that of a traditional method can be achieved only by adopting the mixing length of one mixing period, and thewhole microfluidic concentration gradient chip structure is simplified.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, in particular to an equivalent circuit microfluidic concentration gradient chip with simplified channels. Background technique [0002] Concentration gradients are widely used in high-throughput drug screening, chemotaxis analysis, and toxicity evaluation. In macroscopic devices, due to the flow characteristics of liquids, it is difficult to maintain long-term stability, so it is difficult to maintain long-term stable concentrations. gradient. Microfluidic chip technology solves this problem well. Based on the principle of mixing and diffusion of laminar liquid in microfluidic channels, it can form a stable concentration gradient in microchannels of tens to hundreds of microns. The most classic microfluidic concentration gradient chip design is the Christmas tree model proposed by Professor Whitesides of Harvard University, through the layer-by-layer diffusion and mixing of samples an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/5027
Inventor 石环环周亚诺张嘉豪袁霖余月华陶林节
Owner NANCHANG HANGKONG UNIVERSITY
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