Method for determining monosaccharide composition and content in radix astragali medicinal material
A technology of Astragalus polysaccharides and monosaccharides, which is applied in the field of monosaccharide composition and content measurement in Astragalus medicinal materials, can solve the problems of lack of specific characteristics of indicators, achieve good stability and recovery, good separation effect, and wide linear range.
Inactive Publication Date: 2021-03-16
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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[0004] The purpose of the present invention is to provide a method for measuring the composition and content of monosaccharides in Astragalus medicinal materials, so as to solve the problem that current researchers in the above-mentioned background technology use ultraviolet spectrophotometry to measure the total polysaccharide content as an index for evaluating the quality of medicinal materials. However, this The problem of lack of specific characteristics of indicators
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[0022] see figure 1 , the present invention provides a technical solution: a method for determining the composition and content of monosaccharides in Astragalus medicinal materials, the specific steps of the method for determining the composition and content of monosaccharides in Astragalus medicinal materials are as follows:
[0023] Material selection: 9 batches of Astragalus medicinal materials from Shanxi Province, Gansu Province and Inner Mongolia were collected, and the detailed information is shown in Table 1. Astragalus medicinal material is identified as the dried root of Astragalus medicinal material according to the identification requirements of Part I of Astragalus membranaceus in the 2020 edition of "Chinese Pharmacopoeia".
[0024] Anhydrous glucose (YG8510, Yifeixue Biotechnology Co., Ltd.), rhamnose (YR8080, Yifeixue Biotechnology Co., Ltd.), galactose (YG8010, Yifeixue Biotechnology Co., Ltd.), mannose (YG8420, Yifeixue Biotechnology Co., Ltd.) Feixue Biotec...
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The invention belongs to the technical field of measurement of monosaccharide composition and content in radix astragali medicinal materials, and particularly relates to a method for measuring monosaccharide composition and content in radix astragali medicinal materials, which comprises the following specific steps of S1, extracting radix astragali polysaccharide, S2, hydrolyzing the radix astragali polysaccharide, S3, derivatization of a monosaccharide reference substance, S4, derivatization of the sample, S5, setting chromatographic conditions, S6, inspecting a linear relationship, S7, carrying out a stability test, S8, carrying out a repeatability test, S9, recovery rate and precision testing, and S10, determining the monosaccharide composition and content in the radix astragali polysaccharide. The method is high in sensitivity, good in separation effect, wide in linear range, low in detection limit, good in precision, repeatability, stability and recovery rate, easy and convenientto operate and stable and reliable in result, can be used for monosaccharide composition analysis and quantitative determination of polysaccharide in the radix astragali medicinal material, and can also provide reference for medicinal material plant polysaccharide composition research.
Description
technical field [0001] The invention relates to the technical field of measuring the composition and content of monosaccharides in medicinal materials of Astragalus, in particular to a method for measuring the composition and content of monosaccharides in medicinal materials of Astragalus. Background technique [0002] Astragalus is the dried root of the leguminous plant Astragalus mongolica or Astragalus membranaceus. The former is mainly produced in Northeast China, while the latter is mainly produced in Shanxi, Inner Mongolia, and northern Shaanxi. As one of the commonly used traditional Chinese medicines, Astragalus membranaceus is sweet in taste and flat in nature, and has the functions of invigorating qi and strengthening the surface, diuretic and detoxification, draining pus, astringing sores and promoting muscle growth. Current studies believe that the main chemical components of Astragalus are flavonoids, saponins, and polysaccharides, which have pharmacological eff...
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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 严国俊陆兔林余亦婷谢辉毛春芹成小兰顾薇张倩马丽霞张佳
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE