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Lactobacillus paracasei strain and use thereof

A technology of Lactobacillus and para-cheese, applied in the direction of Lactobacillus, bacteria, application, etc., can solve problems such as intestinal health that have not yet been discovered

Pending Publication Date: 2021-03-19
KO BIOLABS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] However, a strain excellent in improving intestinal health, such as treatment of IBD and IBS, and reducing allergic symptoms has not been found, and many research institutions have been working in order to find a strain having such an effect

Method used

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  • Lactobacillus paracasei strain and use thereof
  • Lactobacillus paracasei strain and use thereof
  • Lactobacillus paracasei strain and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] Example 1. Screening of Probiotic Strains with Immunomodulatory Function

[0115] Probiotic strains with immunomodulatory function were screened using leukemic monocyte THP-1 cell line and peripheral blood mononuclear cells (PBMC). Strains derived from the human gut or vagina, respectively, were inoculated into the above two cell lines so that the ratio of cell line:strain was 1:100, and IL-10 (a major cytokine indicative of inflammation control) and IL-10 were measured. -6 (a major cytokine indicative of an inflammatory response) ratio (IL-10 / IL-6), and the ratio of IL-10 to IFN-γ (a major cytokine indicative of an autoimmune response) (IL -10 / IFN-γ). A total of 23 strains were used for screening, as follows: two strains of Lactobacillus gasseri, one strain of Lactobacillus reuteri, five strains of Lactobacillus rhamnosus, two strains of Lactobacillus fermentum, four strains of para Lactobacillus casei strains, four Lactobacillus salivarius strains, one Lactobacillus...

Embodiment 2

[0119] Example 2. Verification of the effects of Lactobacillus paracasei KBL382, KBL384 and KBL385 strains on inflammatory cytokine expression and T cell differentiation regulation (test in vitro)

[0120] In order to verify the immunomodulatory effect from said Lactobacillus paracasei KBL382, KBL384 and KBL385 strains, the ability to produce cytokines involved in immune regulation was determined in THP-1 cell lines treated with Lactobacillus paracasei KBL382, KBL384 and KBL385 strains , while determining whether marker genes of T cell differentiation were expressed in PBMCs treated with Lactobacillus paracasei KBL382 and KBL385 strains.

[0121] First, the THP-1 cell line was treated with 1×10 5 Cells were seeded into each well of a 24-well plate, differentiated into mature macrophages, and then the culture solution was replaced. After 3 hours, one well was treated with LPS at a concentration of 1 μg / mL as a positive control, and the other wells were treated with Lactobacill...

Embodiment 3

[0139] Example 3. Verification of the effect of Lactobacillus paracasei KBL382, KBL384 and KBL385 strains on T cell immune regulation (test in vitro)

[0140] At the same time, in order to verify the immunomodulatory effect of Lactobacillus paracasei KBL382, KBL384 and KBL385 strains on T cells, when T cells were activated in PBMC cells and were subsequently treated with the paracasei KBL382, KBL384 and KBL385 strains, it was verified that inflammation The expression level of sex cytokines.

[0141] PBMC cells were divided into 2×10 5 Cells were seeded into each well of a 96-well plate, T cells were activated with anti-CD3 antibody (1 μg / ml, ebioscience), and then based on the number of living cells, 1×10 7 Lactobacillus paracasei KBL382, KBL384 or KBL385 strains were added to each cell to make "bacteria:PBMC=50:1". As a control, based on the number of viable cells, 1 x 10 7 2 cells were added to each well of Escherichia coli K12 (ATCC 10798). After incubating the test gro...

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Abstract

The present invention pertains to: a novel human-derived probiotic strain that is Lactobacillus paracasei KBL382, Lactobacillus paracasei KBL384, or Lactobacillus paracasei KBL385; and a use of the strain for improving intestinal health and treating or preventing intestinal diseases. The strain according to the present invention has excellent anti-inflammatory and immunomodulatory functions, has asuperb strengthening effect on tight junctions of the intestinal tract wall, suppresses enteritis-induced weight loss and colon length reduction, thereby exhibiting a therapeutic effect for enteritis, and significantly alleviates the symptoms of atopic dermatitis. Therefore, the strain can be useful as a probiotic material for enhancing anti-inflammatory effects, strengthening immunity, improvingintestinal health functions, and alleviating allergic diseases.

Description

technical field [0001] The present invention relates to Lactobacillus paracasei (Lactobacillus paracasei) bacterial strain and application thereof. More specifically, the present invention relates to health functional food compositions for reducing allergic symptoms, improving immune regulation, reducing inflammatory symptoms, reducing atopic dermatitis and improving intestinal health; and for treating or preventing allergic A pharmaceutical composition for disease, autoimmune disease, inflammatory disease, atopic dermatitis and / or enteropathy, which comprises an effective amount of at least one selected from the following: Lactobacillus paracasei KBL382 strain, Lactobacillus paracasei KBL384 strain , Lactobacillus paracasei KBL385 bacterial strain, the thalline of said bacterial strain, the culture of said bacterial strain, the lysate of said bacterial strain and the extract of said bacterial strain. Background technique [0002] Probiotics refer to microorganisms and the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P1/00A61P29/00A61P37/08C12R1/225
CPCA23L33/135A61K35/747A61P1/00C12R2001/225C12N1/205A61P37/06A61P37/08A23V2400/165C12N1/20A23V2002/00A23V2200/32A23L33/40A61K2035/115
Inventor 高光杓金云基张俞真徐普揽李濬哲南泰旭金因洙李臻雨
Owner KO BIOLABS INC
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