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Method for determining flavonoid components in classic famous prescription Dajianzhong decoction

A technology of Dajianzhong Decoction and flavonoids, which is applied in the field of determination of flavonoids in the classic famous prescription Dajianzhong Decoction, can solve the problems of high proportion of jelly and maltose, interference of content determination of index components in preparations, etc., and improve safety And reliability, stability and repeatability, the effect of improving quality standards

Active Publication Date: 2021-03-30
SICHUAN SUNNYHOPE PHARM CO LTD
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, according to the ancient recipe of Dajianzhongtang (Synopsis of the Golden Chamber), the mass proportion of jelly in Dajianzhongtang preparation is relatively high, which will cause great interference to the determination of the content of index components in the preparation

Method used

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  • Method for determining flavonoid components in classic famous prescription Dajianzhong decoction
  • Method for determining flavonoid components in classic famous prescription Dajianzhong decoction
  • Method for determining flavonoid components in classic famous prescription Dajianzhong decoction

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preparation example Construction

[0036] The preparation method of water-saturated n-butanol is as follows: n-butanol and distilled water are mixed (the mixing ratio is 1:1 by volume), shaken, mixed, and left to separate layers. The upper layer is a water-saturated n-butanol solution, and the lower layer It is an aqueous solution saturated with n-butanol.

[0037] The rutin reference substance was purchased from the China National Institute for Food and Drug Control, with a purity of 91.7%; the hyperoside reference substance was purchased from the China Food and Drug Control Institute, with a purity of 99.41%; the quercetin reference substance was purchased from the China Food and Drug Control Institute, 99.17% purity.

[0038]The Dajianzhongtang preparation used in the embodiments of the present invention is prepared according to the ancient recipe of the classic recipe Dajianzhongtang (Synopsis of the Golden Chamber), and the specific preparation method is as follows: take 9g of Sichuan pepper (to remove swe...

Embodiment 1

[0039] Embodiment 1, the method for qualitative determination of rutin, hyperin and quercetin in Dajianzhong Decoction

[0040] Under the following chromatographic conditions, simultaneously determine whether Dajianzhong Decoction contains rutin, hyperin, and quercetin.

[0041] Preparation of Dajianzhong Decoction test solution: same as in Example 2.

Embodiment 2

[0044] Embodiment 2, the method for quantitative determination of rutin, hyperin and quercetin content in Dajianzhong Decoction

[0045] Taking rutin, hyperin, and quercetin as reference substances, the contents of rutin, hyperin, and quercetin in Dajianzhong Decoction were simultaneously determined under the following chromatographic conditions.

[0046] Preparation of Dajianzhongtang test solution: Take 2g of Dajianzhongtang preparation, add 8ml of pure water, shake to dissolve completely, add water-saturated n-butanol and shake to extract 4 times, add water-saturated n-butanol each time The amount of butanol is 10ml, combine the n-butanol solution, recover the solvent to dryness, dissolve the residue with a small amount of methanol, transfer it to a 2ml volumetric flask, add methanol to the mark, shake well, filter, and take the subsequent filtrate.

[0047] Preparation of reference substance solution: Weigh 112.93 mg of rutin reference substance, 25.22 mg of hyperoside ref...

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Abstract

The invention provides a method for determining flavonoid components in a classic famous prescription Dajianzhong decoction. According to the method, a Dazhuanzhong decoction preparation is used as atest sample, high performance liquid chromatography detection is carried out, and rutin, hyperoside and quercetin in the Dazhuanzhong decoction preparation are determined at the same time. A Dazhuanzhong decoction preparation prepared according to an ancient prescription process of classic famous prescription Dazhuanzhong decoction (Jin Gui Yao Lue) is prepared into a test solution by adopting themethod disclosed by the invention, so that under specific chromatographic conditions, the interference of carbohydrates can be effectively eliminated, and qualitative analysis and quantitative determination can be carried out on a plurality of flavonoid components (rutin, hyperin and quercetin) in the Dazhuanzhong decoction preparation. The method provided by the invention can accurately and comprehensively evaluate the quality of the Dazhuanzhong decoction, is simple, convenient and rapid, has good stability and repeatability, is beneficial to improving the quality standard of the Dazhuanzhong decoction and improving the safety and reliability of medicines, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of quality control of traditional Chinese medicines, and in particular relates to a method for determining flavonoid components of a classic famous prescription Dajianzhong Decoction. Background technique [0002] Dajianzhong Decoction is from "Golden Chamber Syndrome (Han·Zhang Zhongjing) Tenth Syndrome and Treatment of Abdominal Fullness, Cold, Hernia, Suspension, Food and Disease": Great cold and pain in the heart and chest, vomiting and unable to eat, cold in the abdomen, rash on the upper part of the skin, and a head when it comes out Feet, pain up and down and cannot be touched, Dajianzhong decoction is the main one. Dajianzhong Tang Recipe: two ounces of Sichuan pepper (to remove sweat), four liang of dried ginger, two liang of ginseng; Take half a liter of porridge and take it at a different temperature. Eat the chyme one day and warm it. Dajianzhong Decoction mainly treats the symptoms of fullness and pain...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 沈立杨军杨安东杨强芮旭东程亮胥勤
Owner SICHUAN SUNNYHOPE PHARM CO LTD
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