System for expanding propagation of arbuscular mycorrhizal fungi with alfalfa as host

Abstract

The invention discloses a system for expanding propagation of arbuscular mycorrhizal fungi with alfalfa as a host. The system comprises a plurality of fixed stand columns, a plurality of fungus expanding propagation devices are arranged on each fixed stand column at intervals in the height direction of the fixed stand column, each fungus expanding propagation device comprises an inner planting cavity and an outer planting cavity, and a fungus expanding propagation cavity is formed between each inner planting cavity and the corresponding outer planting cavity; and the two planting cavities communicate with the corresponding fungus expanding propagation cavity through a plurality of fungus propagation diffusion channels, so that fibrous roots and arbuscular mycorrhizal fungi of the alfalfa are propagated into the fungus expanding propagation cavities. According to the system, arbuscular mycorrhizal fungi and some fibrous roots can be introduced into fungus matrix soil in the fungus expanding propagation cavities through the fungus propagation diffusion channels, hyphae, spores and the like are attached into the fungus matrix soil, in this way, after the fungus matrix soil is collected, an arbuscular mycorrhizal fungus inoculant is prepared, then the fungus expanding propagation cavities are filled with new fungus matrix soil again, the growth of alfalfa root systems and the growth and propagation of fungi are not influenced, and the expanding propagation efficiency of arbuscular mycorrhizal fungi is improved while continuous production is realized.

Description

technical field [0001] The invention relates to the propagation of arbuscular mycorrhizal fungi in the field of microorganisms, in particular to a system for propagating arbuscular mycorrhizal fungi with alfalfa as a host. Background technique [0002] Mycorrhizae are the result of the co-evolution of plants and mycorrhizal fungi during their long-term survival. Its existence is not only conducive to improving the ability of plants to resist adverse environments, promoting plant growth, but also conducive to the survival of mycorrhizal fungi. This relationship sometimes develops to the point that the two sides are inseparable. Plants cannot survive without mycorrhizae, and mycorrhizal fungi cannot complete their life cycle and continue to reproduce without the necessary plant root symbiosis. [0003] Arbuscular mycorrhiza is the most common type of endomycorrhizal, which is a symbiont formed between an arbuscular mycorrhizal fungus in soil and plant roots. Arbuscular mycor...

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Problems solved by technology

[0005] However, arbuscular mycorrhizal fungi are strictly symbiotic fungi, and their survival is strictly dependent on living higher plants, so it is difficult to cultivate and produce arbuscular mycorrhizal fungal agents on a large scale

At present, there are many methods to obtain arbuscular mycorrhizal fungi in the laboratory, such as: medium culture method, static nutrient solution culture method, flowing nutrient solution culture method, spray liquid culture method, arbuscular mycorrhizal fungi and plant isolated roots The aseptic double culture method of organs and the glass bead compartment culture method, etc. These culture methods generally use clover as host plants for cultivation, but the root system of clover is relatively small, and it is an annual herb with a short life cycle. Proliferating arbuscular mycorrhizal fungi difficult for long-term industrialization

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