Glucomannan series composition, preparation method and application thereof
A technology of glucomannan oligosaccharide and composition, applied in the field of enzymatic degradation, can solve the problems of unstable process, complicated post-treatment process of acid degradation means, severe reaction conditions, etc., and achieve the effect of significant anti-inflammatory biological activity
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Embodiment 1
[0043] This embodiment provides a method for degrading glucomannan, and the specific steps are as follows:
[0044] Fully dissolve Bletilla striata polysaccharide weighing 5 g in 200 ml deionized water, heat to 60° C., and stir for 24 hours. The bletilla striata polysaccharide solution is freeze-dried to obtain the bletilla striata polysaccharide freeze-dried powder. Weigh 500 mg of bletilla striata polysaccharide freeze-dried powder, dissolve it in 150 ml of deionized water, and place it in a round bottom flask. Then weigh 50mg combined enzymes, including in mass fraction: 5% endo-1,4-β-mannanase, 5% β-mannosidase, 5% β-glucosidase and 85% cellulase , the combined enzymes were fully dissolved in a round bottom flask at the same time, heated to 40°C, and the reaction time was 24 hours, and the combined enzymes degraded the polysaccharides of Bletilla striata. After the reaction is over, heat to 100° C. for 10 minutes to inactivate the enzyme so as to terminate the enzyme deg...
Embodiment 2
[0046] This embodiment provides a method for degrading glucomannan, and the specific steps are as follows:
[0047] Fully dissolve 5 g of natural konjac polysaccharide in 200 ml of deionized water, heat to 60° C., and stir for 24 hours. The konjac polysaccharide solution is subjected to freeze-drying operation to obtain konjac polysaccharide freeze-dried powder. Weigh 500mg konjac polysaccharide freeze-dried powder and dissolve it in 150ml deionized water, and place it in a round bottom flask. Then weigh 50mg combined enzymes, including in mass fraction: 10% endo-1,4-β-mannanase, 10% β-mannosidase, 10% β-glucosidase and 70% cellulase , the combined enzymes were fully dissolved in the round bottom flask at the same time, heated to 40°C, and the reaction time was 24h, and the combined enzymes were used to degrade konjac polysaccharides. After the reaction is over, heat to 100° C. for 10 minutes to inactivate the enzyme so as to terminate the enzyme degradation reaction. Then ...
Embodiment 3
[0049] This embodiment provides a method for degrading glucomannan, and the specific steps are as follows:
[0050] Fully dissolve 5 g of natural konjac polysaccharide in 200 ml of deionized water, heat to 60° C., and stir for 24 hours. The konjac polysaccharide solution is subjected to freeze-drying operation to obtain konjac polysaccharide freeze-dried powder. Weigh 500mg konjac polysaccharide freeze-dried powder and dissolve it in 150ml deionized water, and place it in a round bottom flask. Then weigh 50mg combined enzymes, including in mass fraction: 10% endo-1,4-β-mannanase, 10% β-mannosidase, 70% β-glucosidase and 10% cellulase , the combined enzymes were fully dissolved in the round bottom flask at the same time, heated to 40°C, and the reaction time was 24h, and the combined enzymes were used to degrade konjac polysaccharides. After the reaction is over, heat to 100° C. for 10 minutes to inactivate the enzyme so as to terminate the enzyme degradation reaction. Then ...
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Abstract
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