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A Pandoraea pnomenusa S2-2 bacterial strain and its application

A pandorae, gdmccno technology, applied in the field of Pandorae apnomenusa S2-2 strains, can solve the problem of limited types of polychlorinated biphenyl degrading bacteria and the like, and achieve the effect of good application prospects

Active Publication Date: 2022-06-21
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to overcome the problem of the limited species of existing PCB-degrading bacteria, and provide a Pandoraea pnomenusa S2-2 bacterial strain with PCB-degrading ability

Method used

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  • A Pandoraea pnomenusa S2-2 bacterial strain and its application
  • A Pandoraea pnomenusa S2-2 bacterial strain and its application
  • A Pandoraea pnomenusa S2-2 bacterial strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Screening of strain S2-2

[0032] (1) Preparation of selective separation medium:

[0033] Mixture44 solution (mg / 100mL): Na 2 B 4 O 7 ·10H 2 O 17.7, CuSO 4 ·5H 2 O 39.2, CaCl 2 ·6H 2 O20.1, ZnSO 4 ·7H 2 O 1095, EDTA 250, add 100-150 μL concentrated H 2 SO 4 , to prevent precipitation.

[0034] Trace metal salt solution (mg / 100mL): EDTA 0.5, CaCO 3 1. FeSO 4 ·7H 2 O 0.5, MgSO 4 ·7H 2 O10, MnSO 4 ·H 2 O 10, Mixture 44 10 mL.

[0035] Macroelement solution (g / L): (NH 4 ) 2 SO 4 1. KH 2 PO 4 3. Na 2 PO 6, trace metal salt solution 0.5 mL, adjusted to pH 7.1 (adjusted by dropwise addition of dilute HCl and NaOH).

[0036] MM30 liquid medium: (NH 4 ) 2 SO 4 g / L, 1KH 2 PO 4 3g / L, Na 2 PO 4 6g / L, trace metal salt solution 0.5mL, pH 7.1 (When preparing MM30 medium, add vitamin B12 0.0002mg / L to induce microbial dechlorination.)

[0037] MM30 solid medium: add 1.8% agar to MM30 liquid medium, sterilize by high pressure steam (121℃,...

Embodiment 2

[0051] Example 2 Identification of strain S2-2

[0052] 1. Traditional biological identification

[0053] (1) Appearance characteristics of strain S2-2

[0054] During the passaging process, the growth on MM30 medium was relatively slow, and S2-2 formed a slightly transparent colony with the size of a needle tip visible to the naked eye about 72 hours after inoculation, such as figure 1 shown.

[0055] On LB solid medium, S2-2 showed translucent spherical colonies visible to the naked eye after 72h-96h, such as figure 2 shown.

[0056] image 3 It is a Gram-stained microscope image of S2-2 (magnification 1000X), S2-2 is a Gram-negative strain, and long pink rods are observed, which are mostly arranged in chains or Y-shaped.

[0057] Figure 4 It is the scanning electron microscope image of S2-2. The morphology of the strain was observed under the electron microscope at 5K and 45K respectively. It was found that S2-2 was a slender rod-shaped bacteria under the electron m...

Embodiment 3

[0071] Example 3 Verification of the degradation system of PCB3 (4-chlorobiphenyl) by PS2-2

[0072] (1) Reagents

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Abstract

The invention discloses a Pandoraea pnomenusa S2-2 strain and its application. The strain was preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on November 12, 2020, and the strain preservation number is GDMCC No: 61284 . The research of the present invention shows that the Pandoraea pnomenusa S2-2 bacterial strain has the effect of efficiently degrading polychlorinated biphenyls, and the Pandoraea pnomenusa S2-2 bacterial strain is an effective and green PCB pollution-degrading bacteria, which can repair PCB environmental pollution , has good potential and application prospects.

Description

technical field [0001] The present invention relates to the technical field of microbial degradation, and more particularly, to a strain of Pandoraeapnomenusa S2-2 and its application. Background technique [0002] With the rapid development of industrial modernization, the natural environment is inevitably polluted by various industrial wastes. Polychlorinated biphenyls (PCBs) is one of them. As early as 1881, German scientists Sehmid and Sehult synthesized PCBs in the laboratory for the first time. In 1929, the United States began to produce PCBs for the first time, and then various countries began the commercialization and large-scale production of PCBs, which were widely used in industrial and agricultural production. PCBs have many excellent properties such as chemical inertness, thermal stability, low electrical conductivity and flame retardancy, and are highly hydrophobic and insoluble in water and various organic solvents. They are widely used in the manufacture of h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/22
CPCC12N1/20A62D3/02A62D2101/22
Inventor 陈进军聂芳红陈蒙蒙杨俊玲照那木拉陈志宝林红英刘素青康丹菊朱棣华何敏
Owner GUANGDONG OCEAN UNIVERSITY
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