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High-temperature-resistant nitrosomonas nitrosa strain and application thereof in sewage treatment

A technology of nitrosomonas and sewage treatment, which is applied in biological water/sewage treatment, water/sludge/sewage treatment, polluted groundwater/leachate treatment, etc., which can solve the problems of rapid growth, bacterial reproduction and Population expansion and other issues, to achieve the effect of efficient deamination

Active Publication Date: 2021-04-09
湖北宸微生物科技有限责任公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, Nitrosomonas is sensitive to high temperature, and the suitable temperature of most Nitrosomonas is not higher than 35°C. So far, there are only 2 cases of heat-resistant AOB pure strains reported, but these two strains Both can only have certain ammonia oxidation activity under high temperature conditions, but cannot grow rapidly, that is, high temperature will limit the reproduction and population expansion of these two strains

Method used

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  • High-temperature-resistant nitrosomonas nitrosa strain and application thereof in sewage treatment
  • High-temperature-resistant nitrosomonas nitrosa strain and application thereof in sewage treatment
  • High-temperature-resistant nitrosomonas nitrosa strain and application thereof in sewage treatment

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Experimental program
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Effect test

Embodiment 1

[0018]200 μL was placed in 5 mLHNM medium in 5 mlHNM medium in Hubei Province, Hubei Province, and the 190rpm shaker was incorporated in 27 ° C. Single colonies were obtained on the medium (an agar content of 1.5%).

[0019]The genomic DNA of the separated strain was extracted by Genomic Rapid Bacterial Genomic DNA ISOLATION KIT (Shanghai Life), and 16S RDNA was amplified by PCR and the molecular biological identification was sequenced. Upstream primers used in PCR amplification, downstream primers are:

[0020]Upstream primers 27F: 5'-aga-gtttgatcatggctcAg-3 ', (SEQ ID NO.1)

[0021]Downstream primers 1492R: 5'-TAC-GGTTACCTTGTTCGACTT-3 '. (SEQ ID NO.2)

[0022]The purification and sequencing of the PCR product is completed by Shanghai's life, sequencing results are shown in SEQ ID NO.3. 16SRDNA sequencing results show that the separated strain is nitrosated by nitrosated monocensis (Nitrosomonas EUTROPHA ), Named it is nitrosated monochrobacterium WH-1. The strain is deposited in China's Typic...

Embodiment 2

[0024]The vaccination ring was used to pick 2 ~ 3 cycloal nitroximedoma WH-1 single bacteria to 50 ml of liquid HNM medium, 39 ° C, 160 rpm shaker was cultured after 7 days, the strain suspension (this suspension is defined as WH- 1 inoculating liquid). 50 ml of nitrosated monocansis WH-1 strain was then inoculated into 1 l HNM medium, 39 ° C, 160 rpm shaker for 72 hours, resulting in inoculating liquid. The inoculation fluid in the following examples is obtained by this condition.

[0025]figure 1 The method of implementation is that the pre-prepared WH-1 bacterial inoculation solution is inoculated into 2000 ml of HNM medium by 5%, and then dispensing it into a 250 mL conical bottle (100 mL per bottle). At 32 ° C, 36 ° C, 39 ° C, 42 ° C, and 45 ° C, 160 r · min-1Shalling bed culture 40 h, periodic nitrogen nitrogen concentration (D, Mg · L)-1), And calculate the growth rate (μ, h)-1) And extraction (T, H): μ = (LNVn-LNVn-1) / 24, where VnAccumulation speed (mg · L)-1· H-1), Its calcu...

Embodiment 3

[0028]figure 2 The implementation method is: pre-prepared WH-1 bacterial inoculation fluid is inoculated into 1000 ml of domestic sewage by 2.5%, and then dispensing it into a 250 mL conical bottle (100 ml per bottle). Under 40 ° C and 45 ° C, 160R · min-1Shalting bed culture 96h, the control group was not angiite, and the nitrogen nitrogen concentration (D, Mg · L) was determined during the period.-1), And calculate the growth rate (μ, h)-1) And extraction (T, H): μ = (LNVn-LNVn-1) / 24, where VnAccumulation speed (mg · L)-1· H-1), Its calculation method is Vn(D)t -Dt-1) / 24; t = ln2 / μ.

[0029]according tofigure 2 : It can be seen from 40 ° C to 45 ° C, after investing WH-1 bacteria, there is a significant ammonia oxidation effect (nitrogen accumulation speed is up to 2.0 mg · L)-1· H-1), The shortest generation of 40 ° C is 11.9 h, and the shortest generation at 45 ° C can also reach 13.6 h, indicating that the bacteria can grow rapidly and deactivated in high temperature treasch...

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Abstract

The invention relates to the field of microbiological sewage treatment, in particular to a high-temperature-resistant nitrosomonas nitrosa strain and an application thereof in sewage treatment. The bacterial strain is preserved in the China Center for Type Culture Collection on October 6, 2020, the classification name of the bacterial strain is Nitromonas nitrosa strain WH-1, and the preservation number of the bacterial strain is CCTCC NO: M 2020526. The nitrosomonas nitrosa strain can quickly grow and be deaminized at 40 DEG C under an aerobic condition, and is suitable for landfill leachate, printing and dyeing wastewater, black and odorous river water, black and odorous lake water, freshwater aquaculture water and the like.

Description

Technical field[0001]The present invention relates to the field of microbial treatment sewage, and more particularly to high temperature nitrosated monocensococcus and its applications in sewage treatment.Background technique[0002]Ammonia nitrogen is a common contaminant in sewage and pollutant surface water, while ammonia oxidation (microorganisms oxidation of ammonia nitrogen as nitrogen) is the most efficient, most commonly used deamination mechanism, and current sewage biochemical treatment systems, mainly ammonia Nitrosated monocensis in the oxidized bacteria assumes the responsibility of ammonia oxidation. However, nitrosated monocensacids are more sensitive to high temperatures, and the suitable temperature of most nitrosated monascuroblasts is not higher than 35 ° C, so far, only 2 cases of heat-resistant AOB pure strains, but these two strains Both can only have certain ammonia oxidation activity under high temperature conditions, but it is not possible to grow rapidly, tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01C02F103/00C02F103/06C02F103/20C02F103/30
CPCC02F3/02C02F3/34C02F2103/007C02F2103/06C02F2103/20C02F2103/30C02F2303/02C12N1/20Y02W10/10
Inventor 程凯高慧娟熊英向斯
Owner 湖北宸微生物科技有限责任公司
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