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A high temperature resistant nitrosomonas and its application in sewage treatment

A nitrosomonas, high temperature resistant technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, polluted groundwater/leachate treatment, etc. population expansion and other problems, to achieve the effect of high-efficiency deamination

Active Publication Date: 2021-10-15
湖北宸微生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, Nitrosomonas is sensitive to high temperature, and the suitable temperature of most Nitrosomonas is not higher than 35°C. So far, there are only 2 cases of heat-resistant AOB pure strains reported, but these two strains Both can only have certain ammonia oxidation activity under high temperature conditions, but cannot grow rapidly, that is, high temperature will limit the reproduction and population expansion of these two strains

Method used

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  • A high temperature resistant nitrosomonas and its application in sewage treatment
  • A high temperature resistant nitrosomonas and its application in sewage treatment
  • A high temperature resistant nitrosomonas and its application in sewage treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Put 200 μL of sewage from the outlet of an aerobic pool in a domestic sewage treatment system in Wuhan City, Hubei Province into 5 mL of HNM medium, enrich and culture it on a shaker at 27°C and 190 rpm for 7 days, and then pass 3 rounds of streaking on the HNM solid A single colony was obtained on the culture medium (1.5% agar content).

[0019] The genomic DNA of the isolated strains was extracted using the Genomic Rapid Bacterial Genomic DNA Isolation Kit (Shanghai Sangon), and the 16S rDNA was amplified by PCR and sequenced for molecular biological identification. The upstream primers and downstream primers used in PCR amplification are:

[0020] Upstream primer 27F: 5'-AGA-GTTTGATCATGGCTCAG-3', (SEQ ID NO.1)

[0021] Downstream primer 1492R: 5'-TAC-GGTTACCTTGTTACGACTT-3'. (SEQ ID NO.2)

[0022] The purification and sequencing of the PCR products were completed by Shanghai Sangong, and the sequencing results are shown in SEQ ID NO.3. 16SrDNA sequencing results s...

Embodiment 2

[0024] Use an inoculation loop to pick 2 to 3 rings of Nitrosomonas WH-1 single colonies into 50mL liquid HNM medium, and culture it on a shaker at 39°C and 160rpm for 7 days to obtain the strain suspension (this suspension is defined as WH- 1 inoculum). Then 50 mL of the N. eutropha WH-1 strain suspension was inoculated into 1 L of HNM medium, and cultured on a shaker at 39° C. and 160 rpm for 72 hours to obtain an inoculum. The inoculation solutions in the following examples were all obtained through this condition.

[0025] figure 1 The implementation method is: after inoculation of WH-1 bacterial inoculum prepared in advance into 2000 mL HNM medium according to the volume ratio of 5%, it is then divided into 250 mL Erlenmeyer flasks (100 mL per bottle), At 32°C, 36°C, 39°C, 42°C and 45°C respectively, 160 r min -1 Cultivate on a shaking table for 40 hours, during which the concentration of nitrous nitrogen (D, mg·L -1 ), and accordingly calculate the specific growth r...

Embodiment 3

[0028] figure 2 The implementation method is: inoculate the WH-1 bacterial inoculum prepared in advance into 1000 mL of domestic sewage according to the volume ratio of 2.5%, and then divide it into 250 mL Erlenmeyer flasks (100 mL for each bottle), respectively. At 40°C and 45°C, 160r·min -1 Cultivate on a shaking table for 96 hours, no bacterial agent was added to the control group, during which the concentration of nitrous nitrogen was measured (D, mg·L -1 ), and accordingly calculate the specific growth rate (μ, h -1 ) and generation time (T, h): μ=(lnV n -lnV n-1 ) / 24, where V n is the accumulation rate of nitrite nitrogen (mg L -1 h -1 ), which is calculated as V n =(D t -D t-1 ) / 24; T = ln2 / μ.

[0029] according to figure 2 : It can be seen that in the range of 40°C~45°C, after adding WH-1 bacteria, there is an obvious ammonia oxidation effect (the maximum accumulation rate of nitrite nitrogen can reach 2.0 mg·L -1 h -1 ), the shortest generation time at...

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Abstract

The invention relates to the field of microbial treatment of sewage, in particular to a high temperature-resistant nitrosomonas and its application in sewage treatment. The strain was deposited in the China Center for Type Culture Collection on October 6, 2020, and its classification is named as Nitrosomonas nitrosa strain WH‑1, the deposit number is CCTCC NO: M 2020526. The above-mentioned Nitrosomonas can grow rapidly and deaminate at 40°C under aerobic conditions, and is suitable for landfill leachate, printing and dyeing wastewater, black and smelly river water, black and smelly lake water, freshwater aquaculture water, etc.

Description

technical field [0001] The invention relates to the field of microbial treatment of sewage, in particular to a strain of high-temperature-resistant nitrosomonas and its application in sewage treatment. Background technique [0002] Ammonia nitrogen is a common pollutant in sewage and polluted surface water, and ammonia oxidation (the process by which microorganisms oxidize ammonia nitrogen to nitrite nitrogen) is the most efficient and most commonly used deamination mechanism. In the current sewage biochemical treatment system, ammonia is mainly Among the oxidizing bacteria, Nitrosomonas is responsible for ammonia oxidation. However, Nitrosomonas is sensitive to high temperature, and the suitable temperature of most Nitrosomonas is not higher than 35°C. So far, there are only 2 cases of heat-resistant AOB pure strains reported, but these two strains Both can only have certain ammonia oxidation activity under high temperature conditions, but cannot grow rapidly, that is, hig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01C02F103/00C02F103/06C02F103/20C02F103/30
CPCC02F3/02C02F3/34C02F2103/007C02F2103/06C02F2103/20C02F2103/30C02F2303/02C12N1/20Y02W10/10
Inventor 程凯高慧娟熊英向斯
Owner 湖北宸微生物科技有限责任公司
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