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Bioactive peptide with amino acid structure AIRNDEELNKLLGR as well as preparation method and application of bioactive peptide

A technology of biologically active peptides and amino acids, applied in the field of protein, to improve quality, reduce the incidence of the body, and promote the effect of lymphocyte proliferation

Active Publication Date: 2021-04-16
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the related functions of Histone H2A type 3 and HistoneH2A type 1-E protein polypeptide fragments in the prior art

Method used

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  • Bioactive peptide with amino acid structure AIRNDEELNKLLGR as well as preparation method and application of bioactive peptide
  • Bioactive peptide with amino acid structure AIRNDEELNKLLGR as well as preparation method and application of bioactive peptide
  • Bioactive peptide with amino acid structure AIRNDEELNKLLGR as well as preparation method and application of bioactive peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The artificial synthesis of eight kinds of bioactive peptides of embodiment 1

[0064] 1. Synthesis of Bioactive Peptides

[0065] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0066] 2. After 2 hours, wash the resin with nitrogen-dimethylformamide (DMF) that is 3 times the volume of the resin, and then drain it. Repeat this four times, and drain the resin for use.

[0067] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0068] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful.

[0069]...

Embodiment 2

[0121] Example 2 The immunomodulatory activity experiment of biologically active peptides

[0122] 1. Determination of the amount of bioactive peptide AIRNDEELNKLLGRVTI that promotes nitric oxide induction in macrophages (Griess method)

[0123] 1. Experimental reagents and instruments:

[0124] Reagents: experimental animal balb / c mice (male 6-8 weeks old) spleen lymphocyte-derived bioactive peptide AIRNDEELNKLLGRVTI; LPS, purchased from Sigma Company; neutral red staining solution, produced by Beyond Biotechnology Research Institute.

[0125] Equipment: LRH-250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150CO 2 Incubator Heraeus; Dragon WellscanMK3 microplate reader Labsystems.

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Abstract

The invention relates to the field of protein, in particular to bioactive peptide with an amino acid structure AIRNDEELNKLLGR as well as a preparation method and application of the bioactive peptide. The bioactive peptide is one or more selected from of the following eight bioactive peptides: AIRNDEELNKLLGRVTI, AIRNDEELNKLLGRVTIAQ, LQLAIRNDEELNKLLGRVTI, LAIRNDEELNKLLGRVTIA, AIRNDEELNKLLGR, AIRNDEELNKLLGRV, AIRNDEELNKLLGRVTIA and LAIRNDEELNKLLGRVTI. Results of in-vitro immunoregulation function experiments show that the eight bioactive peptides have a very good immunoregulation function. The eight bioactive peptides disclosed by the invention have the capabilities of remarkably promoting lymphocyte proliferation, exerting the immune function of an organism, improving the immunity of the organism, promoting the increase of a nitric oxide induction amount of macrophages, reducing the morbidity of the organism and improving the quality of life, and have very important significance for developing foos, health care products and medicines with an immune regulation function.

Description

technical field [0001] The invention relates to the field of protein, especially the bioactive peptide with amino acid structure AIRNDEELNKLLGR and its preparation method and application. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anti-cancer, lowering blood pressure, antibacterial, lowering cholesterol, anti-diabetic and other properties. More than 3000 different bioactive peptides have been reported in the current most authoritative bioactive peptide database BIOPEP-UMW. [0003] Immunomodulatory peptides are a class of bioactive peptides obtained from milk for the first time after the discovery of opioid peptides and their physiological activity has been proved. Immunomod...

Claims

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Application Information

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IPC IPC(8): C07K14/47C12N15/12A61K38/17A61P29/00A61P37/02A61K8/64A61Q19/00A23L33/18
Inventor 张少辉张伯宇占文静
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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