Coronavirus sample preservation solution and application thereof

A coronavirus and preservation solution technology, applied in the field of biological detection, can solve problems such as false negatives, viral RNA degradation, etc., and achieve the effect of maintaining integrity and broad-spectrum inhibition of growth

Pending Publication Date: 2021-04-16
杭州金域医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Improper handling of collected samples during transportation and storage can degrade viral RNA, resulting in false negative results

Method used

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  • Coronavirus sample preservation solution and application thereof
  • Coronavirus sample preservation solution and application thereof
  • Coronavirus sample preservation solution and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0172] The preparation of embodiment 1 coronavirus sample preservation solution

[0173] The formula of the coronavirus sample preservation solution provided in this embodiment is shown in Table 2, and the coronavirus sample preservation solution is prepared according to the formula composition and concentration in Table 2.

[0174] Formulation of table 2 coronavirus sample preservation solution

[0175]

[0176]

Embodiment 2

[0177] Example 2 Coronary virus sample preservation solution compares the preservation effect of the coronavirus sample

[0178] This experiment is divided into 3 groups, is respectively the coronavirus sample preservation solution group prepared in embodiment 1, Hank'S solution group and control group (physiological saline).

[0179] (1) Coronavirus sample preservation solution group: take 20 μl of new coronavirus COVID-19 (titer is 0.1- 1×10 5 copies / ml) is mixed with the coronavirus sample preservation solution prepared in 2ml embodiment 1, and placed at 2-8°C for preservation after mixing. Nucleic acid extraction was carried out within 1-5 days of storage (once a day), and PCR primers specific to the new coronavirus COVID-19 were used for fluorescent quantitative PCR detection.

[0180] (2) Hank'S group: Take 20 μl of the new coronavirus COVID-19 (titer is 0.1- 1×10 5 copies / ml) mixed with 2ml Hank’s preservation solution, mixed well and stored at 2-8°C. Nucleic acid ex...

Embodiment 3

[0187] Example 3 Comparison of preservation effects of different coronavirus sample preservation solutions on coronavirus samples

[0188] Select 4 preservation solutions that have been put on record by the Drug Administration (inactivated preservation solution: Mole Biology and Microbiology WM-1 type; non-inactivated preservation solution: Shengxiang and Microbiology WM-2 type) and the crown prepared in Example 1 The virus sample preservation solution is used to verify and compare the preservation effect of coronavirus samples, and the preservation method is carried out according to the above-mentioned methods for the collection, preservation and transportation of the new crown nucleic acid. After the original sample was collected, it was placed in normal saline, and within 24 hours from the original sample (with a titer of 0.1-1×10 5 IU / ml) Take 20 μl of novel coronavirus COVID-19 and add it to 2ml preservation solution, mix well and store at 2-8°C. After 72 hours of storag...

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Abstract

The invention relates to a coronavirus sample preservation solution and application thereof. The coronavirus sample preservation solution comprises a cell preserving fluid, a cell membrane protective agent, an RNA enzyme inhibitor, a virus metabolism inhibitor, a virus inactivator and a bacteriostatic agent, wherein the virus inactivator is sodium caprylate, and the bacteriostatic agent is Funme polypeptide. The bacteriostatic agent in the preservation solution selects biological Funme polypeptide, growth of various microorganisms can be inhibited in a broad spectrum, nucleic acid detection of coronaviruses is not interfered, the selected virus inactivator does not influence detection of new coronavirus amplification reagents produced by any manufacturer, and RNA in a sample cannot be degraded by RNA enzyme in the preservation and transportation process through the RNA enzyme inhibitor. According to the preservation solution, collected coronavirus samples are comprehensively protected from all links, the samples are kept in an original state in the preservation and transportation process, the originality of the samples can still be kept after the samples are preserved for 5 days at the temperature of 2-8 DEG C, and detection results consistent with those of the original samples are obtained.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a coronavirus sample preservation solution and its application. Background technique [0002] Nucleic acid detection is a key technology for the diagnosis of new coronavirus infection. After the coronavirus infects the human body, the lesions mainly occur in the upper and lower respiratory tracts and lungs. Usually, the collection of throat swabs for nucleic acid testing is a common method in the screening of new crowns. Improper handling of collected samples during transportation and storage can degrade viral RNA, resulting in false negative results. Therefore, how to protect virus sample RNA from degradation during transportation and storage has become a key concern after sample collection. Contents of the invention [0003] Based on this, the object of the present invention is to provide a coronavirus sample preservation solution, which can effectively pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/24
Inventor 冯莉亚刘军权母佳琪黄琦王欢
Owner 杭州金域医学检验所有限公司
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