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SNP Molecular Markers and Applications Related to Green Foot Traits of Class 817 Broiler Chickens

A technology of molecular markers and broiler chickens, applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve problems such as unclear green feet, and achieve the effect of improving profitability

Active Publication Date: 2022-04-01
NANCHANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specific reason for green feet is not clear at present, and there is no specific plan for selecting breeders to produce 100% green feet 817 commercial broiler chickens

Method used

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  • SNP Molecular Markers and Applications Related to Green Foot Traits of Class 817 Broiler Chickens
  • SNP Molecular Markers and Applications Related to Green Foot Traits of Class 817 Broiler Chickens
  • SNP Molecular Markers and Applications Related to Green Foot Traits of Class 817 Broiler Chickens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 selects and stays according to the genotype the parent generation breeder chicken that can produce green-footed offspring

[0021] 1.1 Sample collection and DNA extraction

[0022] 30 parent roosters of the AA, Ross and Kebao populations to be inspected, 0.3ml blood samples of the parent roosters were collected from the subwing vein, placed in a centrifuge tube containing 70 microliters of anticoagulant, and shaken immediately. Store at -20°C.

[0023] Genomic DNA was extracted using phenol-chloroform extraction (Osper F et al., 1998), and the specific steps were as follows:

[0024] (1) Take 30 μL of whole blood and place in a 1.5 mL centrifuge tube, add 470 μL of 1×SET buffer, 12.5 μL of 20% SDS and 6 μL of 10 mg / mL proteinase K, mix well and place in a 55°C water bath overnight;

[0025] (2) Take out the sample in a 1.5mL centrifuge tube, add 500μL saturated phenol, shake gently for 20min, and centrifuge at 10,000rpm for 10min;

[0026] (3) Take the s...

Embodiment 2

[0051] Example 2 According to the genotype identification of the commercial generation "Qingjiao" chickens produced by random male and female parents

[0052] 2.1 Production of commercial substitute broilers

[0053] (1) 36 30-day-old commercial broiler chickens produced by Ross 308♂×Hailan Brown♀;

[0054] (2) 36 30-day-old commercial broiler chickens produced by Kebao♂×Jinghong♀.

[0055] 3.2 PCR and target locus typing

[0056] The specific method is:

[0057] 1) Collection and DNA extraction of samples in each group

[0058] Concrete operation is with embodiment 1.

[0059] 2) PCR and target locus typing

[0060] Concrete operation is with embodiment 1.

[0061] 3) Results

[0062] In the Rose 308♂×Hailan Brown♀ group, there were 6 genotype 1 and 2 genotype 2; in the Kebao♂×Jinghong♀ group, 5 genotype 1 and 1 genotype 2 (Table 6).

[0063] 3.3 Phenotype identification

[0064] The two groups of commercial broiler chickens randomly selected in 3.2 were slaughtered...

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PUM

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Abstract

The invention discloses SNP molecular markers and applications related to the green foot traits of 817 broiler chickens, and relates to the technical field of molecular breeding; the SNP molecular markers include SNP-1 and SNP-2; wherein, the core of the SNP-1 The nucleotide sequence is shown in SEQ ID NO: 1, and the 161st, 223rd, 347th, 376th, 586th and 593th positions are polymorphism sites, and the polymorphisms are C / T, A / G, C / T, A respectively. / G, C / T and A / C; the nucleotide sequence of the SNP-2 is shown in SEQ ID NO: 2, the 67th position is a polymorphism site, and the polymorphism is C / T; the present invention Typing the causative mutation genes related to the formation of the "green-footed" trait can quickly breed 817 broiler hybrid lines with the green-footed trait, without increasing the cost of seed production and without changing the method of seed production. Improve the profitability of commercial broilers by changing the color of the shanks.

Description

technical field [0001] The invention relates to the technical field of molecular breeding, in particular to SNP molecular markers related to the traits of green feet of 817 broiler chickens and their applications. Background technique [0002] "Class 817 broiler chicken" mainly refers to large white-feathered broiler chickens (AA, Ross 308, Kebao and other commercial cocks or parent cocks) as the male parent, and high-yield layer hens as the female parent (Hailan, Jinghong, etc.) Roman, Yisha, etc.) are small white-feathered broiler breeds, which have the characteristics of fast growth, strong disease resistance, and high feed remuneration. Therefore, the 817 seed production model has become the most suitable for commercial broiler chicken production in modern industrialized agriculture. One of the commonly used seed production models / supporting models. [0003] At present, there is a phenomenon in the 817 seed production model, that is, in addition to the "white feet" (yel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 许继国罗小庆崔芳芳郭志鸿罗雯林红艳王建枫范传威张丽佳杨成
Owner NANCHANG NORMAL UNIV