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Application of L-malate in preparation of medicine for preventing and treating hepatic ischemia/reperfusion injury

A technology of liver ischemia and malic acid, applied in the field of biomedicine, to achieve a wide range of clinical application value and the effect of inhibiting hepatic ferroptosis

Pending Publication Date: 2021-04-20
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, so far, there is no report on the role of L-malic acid as the only active ingredient in cell ferroptosis and liver ischemia-reperfusion injury.

Method used

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  • Application of L-malate in preparation of medicine for preventing and treating hepatic ischemia/reperfusion injury
  • Application of L-malate in preparation of medicine for preventing and treating hepatic ischemia/reperfusion injury
  • Application of L-malate in preparation of medicine for preventing and treating hepatic ischemia/reperfusion injury

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1 Animal experiment: Application of L-malic acid in the preparation of drugs for preventing and treating liver ischemia-reperfusion injury

[0027] 1. Materials and methods

[0028] 1.1. Experimental animals

[0029] Six-week-old SPF grade C57BL / 6 male mice were purchased from Jiangsu Jicui Yaokang Biotechnology Co., Ltd. and raised in SPF environment. The standard diet AIN-76A (Research Diets, Inc) was adapted to feeding for 2 weeks, and they were randomly divided into groups according to body weight, with 8-10 animals in each group.

[0030] 1.2. Experimental drugs

[0031] L-malic acid was purchased from Sigma-Aldrich Company (Product No. 02288), with a purity of ≥99%, and was dissolved in normal saline.

[0032] 1.3. Construction of mouse liver ischemia-reperfusion model

[0033] Mice were anesthetized with isoflurane (3% concentration for anesthesia induction, 1.5% concentration for anesthesia maintenance) and fixed on a constant temperature platform at...

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Abstract

The invention discloses application of L-malic acid in preparation of a medicine for preventing and treating liver ischemia-reperfusion injury, and particularly discloses application of the L-malic acid serving as a unique active ingredient in preparation of a medicine or a health care product for preventing or treating the liver ischemia-reperfusion injury. Mouse in-vivo experiments prove that the L-malate can significantly reduce the increase of hepatic injury markers such as alanine aminotransferase, aspartate aminotransferase and lactic dehydrogenase in serum caused by hepatic ischemia / reperfusion, and reduce the hepatic necrosis area; and meanwhile, liver tissue lipid peroxidation and cell ferroptosis caused by ischemia / reperfusion are inhibited, so that hepatic injury is relieved. The invention provides a theoretical basis for treatment of hepatic ischemia / reperfusion injury related diseases, and has clinical application value.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of L-malic acid in the preparation of medicines for preventing and treating liver ischemia-reperfusion injury. [0002] technical background [0003] Ischemia / Reperfusion Injury (Ischemia / Reperfusion Injury, IRI) refers to the recovery of blood circulation after a period of ischemia in tissues and organs, and the phenomenon of cell dysfunction and tissue structure destruction is not alleviated but aggravated. Ischemia-reperfusion injury can occur in many important organs, including the heart, liver, lung, kidney, and gastrointestinal tract. [0004] Hepatic ischemia-reperfusion injury (Hepatic Ischemia / Reperfusion Injury, HIRI) is a common complication in liver surgery such as liver resection and liver transplantation, and is one of the main reasons leading to poor surgical results and failure of liver transplantation. Severe HIRI can lead to massive death o...

Claims

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Application Information

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IPC IPC(8): A61K31/194A61P1/16A61P9/10
Inventor 方学贤余盈盈梁静静
Owner HANGZHOU NORMAL UNIVERSITY
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