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Method for green recovery of adenine from adenosine crude product mother liquor

A technology of adenosine crude product and adenosine mother liquor, applied in the direction of organic chemistry, etc., can solve the problems of high overall production cost of adenosine, no progress in recycling of adenosine crude product mother liquor, waste of mother liquor, etc.

Inactive Publication Date: 2021-04-20
HENAN JULONG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the downstream production process of adenosine has not yet achieved significant improvement, especially in the recycling and utilization of the crude adenosine mother liquor. A large amount of mother liquor is wasted, resulting in low overall yield in the downstream section, and the overall cost of adenosine production is relatively high. sufficient market competitiveness

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Pretreatment of adenosine mother liquor

[0025] Pass 30L adenosine mother liquor (adenosine concentration is 28.73g / L, total glycosides 861.9g) through 500 molecular weight polyethersulfone nanofiltration membrane for nanofiltration and collect the supernatant. The working pressure of nanofiltration is 1-1.5Mpa. The temperature is 35-40°C. When the volume of the nanofiltration concentrated liquid reaches 8L, add purified water to the nanofiltration concentrated liquid. The speed of replenishing water is equivalent to the outflow speed of the clear liquid. When the concentration of adenosine in the filtrate was lower than 2.0g / L, the nanofiltration was stopped to obtain 58L of nanofiltration liquid, the concentration of adenosine was 14.52g / L, the total amount was 841.9g, and the pretreatment yield was 97.68%.

[0026] 2. Concentrate the nanofiltration solution under reduced pressure

[0027] Concentrate the pretreated nanofiltration liquid with a rotary evaporator ...

Embodiment 2

[0036] 1. Pretreatment of adenosine mother liquor

[0037] Pass 30L adenosine mother liquor (adenosine concentration is 30.95g / L, total glycosides 928.5g) through 500 molecular weight polyethersulfone nanofiltration membrane for nanofiltration and collect the clear liquid. The working pressure of nanofiltration is 1-1.5Mpa. The temperature is 35-40°C. When the volume of the nanofiltration concentrated liquid reaches 8L, add purified water to the nanofiltration concentrated liquid. The speed of replenishing water is equivalent to the outflow speed of the clear liquid. When the concentration of adenosine in the filtrate was lower than 2.0g / L, the nanofiltration was stopped to obtain 60.2L of nanofiltration liquid, the concentration of adenosine was 15.12g / L, the total amount was 910.12g, and the pretreatment yield was 98.02%.

[0038] 2. Concentrate the nanofiltration solution under reduced pressure

[0039] Concentrate the pretreated nanofiltration liquid with a rotary evapora...

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PUM

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Abstract

The invention relates to a method for green recovery of adenine from adenosine crude product mother liquor, which belongs to the technical field of microbial fermentation downstream, and comprises the following five steps: pretreatment, reduced pressure concentration, enzyme conversion, cooling crystallization and crude product refining, wherein the enzyme conversion is to add adenosine hydrolase into the obtained concentrate according to 30u / g adenosine to convert the concentrate into adenine, according to the method, the adenosine mother liquor is extracted from adenine, enzyme conversion conditions, refining conditions and the like are optimized, then the adenosine mother liquor can be indirectly recycled by utilizing the solubility difference of adenine and adenosine, the obtained product is high in yield and purity, no toxic or harmful substances are generated, green recycling is realized, and the method has huge application value.

Description

technical field [0001] The invention belongs to the downstream technical field of microbial fermentation, and in particular relates to a method for green recovery of adenine from crude adenosine mother liquor. Background technique [0002] Adenosine (Adenosine), that is, adenosine nucleoside, chemical name 9-β-D-ribofuranosyl adenine, molecular formula C 10 h 13 N 6 o 4 , White or off-white powder with a relative molecular mass of 267.24. Adenosine plays important roles in biochemistry, including energy transfer in the form of adenosine triphosphate (ATP) or adenosine diphosphate (ADP), or signaling in the form of cyclic adenosine monophosphate (cAMP). In addition, adenosine is also an inhibitory neurotransmitter (inhibitory neurotransmitter), may promote sleep. So far, adenosine has been widely used in chemical industry, food, medicine, daily chemical and other fields, so it can be seen that this compound has very broad application prospects. [0003] As the scope of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D473/34
Inventor 李静赵鹏飞谢沛
Owner HENAN JULONG BIOLOGICAL ENG CO LTD
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