Preparation and application of electrochemiluminescence sensor for detecting sialylated sugar chain antigen KL-6 iridium nanodots

A sugar chain antigen, sialylation technology, applied in the field of nanomaterials, can solve the problems of easy agglomeration, poor water solubility, limited application, etc.

Active Publication Date: 2021-04-20
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Ir(ppy) 2 (acac) has poor water solubility, easy aggregation, and poor biocompatibility, which greatly limit its application in aqueous immunoassays

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1. Preparation and application of an electrochemiluminescent sensor for detecting sialylated sugar chain antigen KL-6 iridium nano-dots, the steps of the iridium nano-dot solution are as follows:

[0032] At room temperature, 10 mg of bis(2-phenylpyridine) iridium acetylacetonate Ir(ppy) 2 The (acac) solid was dissolved in 1 mL of tetrahydrofuran to prepare a solution with a mass concentration of 10 mg / mL; the sodium hydroxide solution with a molar concentration of 0.1 mol / L and the apoferritin solution with a mass concentration of 0.5 mg / mL were Shake evenly according to the volume ratio of 1:100, and keep stirring at room temperature for 2 h to completely dissociate the structural unit of apoferritin into free subunits; then, add 0.5 mL of iridium with a mass concentration of 10 mg / mL dropwise After stirring the complex solution continuously for 2 h, quickly add 0.1 mL of hydrochloric acid solution with a molar concentration of 0.1 mol / L to the mixed soluti...

Embodiment 2

[0033] Embodiment 2. Preparation and application of an electrochemiluminescent sensor for detecting sialylated sugar chain antigen KL-6 iridium nano-dots, the preparation steps of the iridium nano-dot solution are as follows:

[0034] At room temperature, 15 mg of bis(2-phenylpyridine) iridium acetylacetonate Ir(ppy) 2 (acac) was dissolved in 2mL of tetrahydrofuran to prepare a solution with a mass concentration of 7.5 mg / mL; the sodium hydroxide solution with a molar concentration of 0.2 mol / L and the apoferritin solution with a mass concentration of 0.7 mg / mL were mixed according to volume Shake evenly at a ratio of 1:100, and continue to stir at room temperature for 3 h to completely dissociate the structural unit of apoferritin into free subunits; then, add 1 mL of iridium complex with a mass concentration of 7.5 mg / mL dropwise solution, after stirring continuously for 3 h, quickly add 0.2 mL of hydrochloric acid solution with a molar concentration of 0.2 mol / L to the mixe...

Embodiment 3

[0035] Embodiment 3. Preparation and application of an electrochemiluminescence sensor for detecting sialylated sugar chain antigen KL-6 iridium nano-dots, the preparation steps of the iridium nano-dot solution are as follows:

[0036]At room temperature, 20 mg of bis(2-phenylpyridine) iridium acetylacetonate Ir(ppy) 2 (acac) was dissolved in 4mL tetrahydrofuran to prepare a solution with a mass concentration of 5 mg / mL; the sodium hydroxide solution with a molar concentration of 0.3 mol / L and the apoferritin solution with a mass concentration of 5 mg / mL were mixed according to volume Shake evenly at a ratio of 1:100, and continue to stir at room temperature for 4 h to completely dissociate the structural unit of apoferritin into free subunits; then, add 1.5 mL of iridium complex solution with a mass concentration of 5 mg / mL dropwise, After stirring continuously for 4 h, quickly add 0.3 mL of hydrochloric acid solution with a molar concentration of 0.3 mol / L to the mixed solut...

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PUM

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Abstract

The invention relates to preparation and application of an electrochemiluminescence sensor for detecting sialylated sugar chain antigen KL-6 iridium nanodots, and is characterized in that the iridium nanodots with good water solubility, good biocompatibility and high electrochemiluminescence efficiency are prepared, an antibody labeled iridium nanodot solution is used as a signal probe to develop the label-free electrochemiluminescence immunosensor, the sensor is used for rapid and sensitive detection of the interstitial lung disease marker sialylated sugar chain antigen KL-6, the detection limit is 45 fg/mL, the linear range is 100 fg/mL and 50 ng/mL, and the sensor has the advantages of being high in specificity, good in reproducibility, stable in signal and the like.

Description

technical field [0001] The invention belongs to the field of nanometer materials and the field of biosensing technology. Background technique [0002] As a research hotspot arising from the intersection of biology, chemistry, medicine, electronic technology and other disciplines, electrochemiluminescence immunoassay is an organic combination of electrochemiluminescence technology and immunoassay method, and the prepared immunosensor has low cost , good selectivity, high sensitivity, fast analysis speed, easy automation, miniaturization and integration, etc., have been widely used in disease marker analysis, food safety analysis, environmental pollution analysis and other fields. [0003] Interstitial lung disease has the characteristics of a wide range of patients, strong concealment, long course of disease, complex pathogenesis, and difficulty in diagnosis and treatment. Such diseases can lead to lung organ damage and corresponding dysfunction, which has become a hot and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N21/76G01N33/53G01N33/531G01N33/543B82Y15/00
CPCY02A50/30
Inventor 杜宇任祥吴丹刘雪静范大伟魏琴鞠熀先
Owner UNIV OF JINAN
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