A kind of aniline efficient degrading bacteria and its application
A technology for efficiently degrading bacteria and aniline, applied in the field of environmental microorganisms, can solve the problems of unsatisfactory degradation effect of aniline-degrading bacteria, decreased degradation rate, low aniline concentration, etc. Effect
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Embodiment 1
[0020] Example 1 Isolation and purification of strains
[0021] The isolation and purification steps of strain BA-7 are as follows:
[0022] (1) Domestication and enrichment
[0023] 50ml of activated sludge was added to a 1L acclimation device containing a basic culture solution with aniline as the only carbon source. The activated sludge came from a biochemical tank in a sewage treatment plant in Jiangsu for long-term treatment of aniline wastewater. The formula of basal culture medium is: aniline 0.5g / L, NH 4 Cl 0.5g / L, KH 2 PO 4 1g / L, Na 2 HPO 4 1g / L, MgCl 2 0.02g / L, CaCl 2 0.03g / L. Introduce the air for acclimation and culture for about 15-20 days, and regularly monitor the aniline concentration and sludge growth during the period. When it is detected that the aniline is completely degraded, it is added in time, and the concentration of aniline is gradually increased to 5 g / L, that is, the enriched solution of aniline-degrading bacteria is obtained.
[0024] (2...
Embodiment 2
[0028] Example 2 Identification of strains
[0029] The purified aniline degrading bacteria were subjected to 16s rDNA sequencing, and the sequencing comparison result was Bacillus albicans ( Bacillus albus ), designated BA-7. Its main physiological characteristics are positive Gram staining, aerobic growth, and the ability to use aniline as the only carbon source and energy source for growth. Take a few bacterial samples for crystal violet staining. Under the x1000 magnification oil microscope, it was observed that the bacteria were rod-shaped and had spores. The physiological and biochemical identification of the strains was carried out in accordance with the "Common Bacterial System Identification Manual". The microscopic examination photos are attached. figure 1 shown.
Embodiment 3
[0030] Example 3 Growth curve of strain BA-7 and degradation experiment of p-aniline
[0031] Pick the single colony of the purified aniline degrading bacteria BA-7 in the present invention in a shake flask containing 5g / L aniline of an inorganic salt medium, and the inorganic salt medium formula contains: NH 4 Cl 0.5g / L, KH 2 PO 4 1g / L, Na 2 HPO 4 1g / L, MgCl 2 0.02g / L, CaCl 20.03g / L. The shaking speed was 180 rpm, the temperature was 37 °C, and the shaking was performed. Samples were taken at 0h, 6h, 12h, 18h, 24h, 30h, 36h, 42h, 48h, 60h, and 72h to determine the OD600 and aniline concentration of bacteria. OD600 was measured by visible light spectrophotometer, and aniline concentration was measured by liquid chromatography. Data as attached figure 2 As shown, at the initial aniline concentration of 5 g / L, the growth of strain BA-7 was slow within 12 h, resulting in slow degradation of aniline. The removal rate reached 50%. When cultured for 48h, aniline has reac...
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