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SNP molecular marker for ocular muscle depth character of large white pig and application

A molecular marker and porcine eye muscle technology, which can be used in proteomics, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problem of high cost

Active Publication Date: 2021-04-23
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity of the formation mechanism of eye muscle depth traits, and the fact that it can only be measured after slaughter and the cost is high, and there may be unfavorable and antagonistic correlations with other traits, it is directly difficult for traditional breeding methods to detect eye muscle depth. Great genetic progress has been made in depth (Ni Debin et al., Research on the measurement method of backfat thickness and eye muscle area (B-ultrasound) in live pigs [J]. Breeding and Feed, 2015(01):5-9.)

Method used

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  • SNP molecular marker for ocular muscle depth character of large white pig and application
  • SNP molecular marker for ocular muscle depth character of large white pig and application
  • SNP molecular marker for ocular muscle depth character of large white pig and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Genotyping Detection

[0030] (1) Take an appropriate amount of tissue samples into a 1.5mL centrifuge tube.

[0031] (2) Add 500 μL of lysate and 5 μL of proteinase K (20 mg / mL) to the centrifuge tube, shake and mix for 30 seconds, place in a 65°C oven or metal bath, and lyse for 30 minutes to 1 hour.

[0032] (3) After the lysis is complete, transfer all the supernatant to a deep-well plate (marked as ①), and add 350 μL of isopropanol to each well.

[0033] (4) Put the No. ① deep-well plate on station 1 of the nucleic acid extractor, and place the deep-well plates with magnetic beads, washing solution ①, washing solutions ② and ③ and eluent on station 2~ 6 on. Turn on the power of the instrument, and set the parameters as shown in Table 1 after the instrument completes the self-test.

[0034] Table 1 Instrument parameter settings

[0035]

[0036] (5) Run the program. After the program ends, the instrument will automatically stop, and station 6 will ...

Embodiment 2

[0040] Example 2: Application of the INRA0052808 molecular marker typing method in the correlation analysis of eye muscle depth traits in American Large White pigs

[0041] Phenotype pretreatment of American large white eye muscle depth traits:

[0042] The eye muscle depth traits of American large white pigs need to use the mixed linear model (MLM), using the joint effect of field, year and season, gender as a fixed effect, individual as a random effect, and body weight as a covariate to calculate the individual random effect. As newly constructed phenotypes (pseudo-phenotypes) for subsequent GWAS analysis. The DMUAI module under the DMU statistical environment was used for restricted maximum likelihood estimation (restricted maximum likelihood, REML) and BLUP analysis, and the data contained 16534 individuals. The specific model is as follows:

[0043] the y ijklm =μ+HYS i +Sex j +ID l +Weight k +ε ijklm

[0044] Among them, y ijklm is the original phenotype value ...

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Abstract

The invention belongs to the technical field of animal molecular marker preparation, and particularly relates to an SNP molecular marker for ocular muscle depth character of a large white pig and an application. The molecular marker is cloned from a gene segment with a self-login number of INRA0052808. The gene segment is subjected to typing screening through a gene chip technology to obtain the SNP marker associated with the ocular muscle depth character of the large white pig, the nucleotide sequence of the SNP marker is shown as SEQ ID NO: 1, A / G allele mutation exists at the 101st basic group of the shown sequence, and when the 101st nucleotide of the sequence is A, it is determined that the large white pig has a higher ocular muscle depth. The invention provides a new genetic resource for marker-assisted selection of the ocular muscle depth character of the large white pig.

Description

technical field [0001] The invention belongs to the technical field of preparation of animal molecular markers, and in particular relates to a SNP molecular marker and application of large white pig eye muscle depth traits. The molecular marker of the present invention can be used for molecular marker-assisted selection of eye muscle depth traits in Large White pigs. Background technique [0002] Pig carcass traits include backfat thickness, eye muscle area and carcass lean meat percentage, etc. These are important economic traits of pigs, which are closely related to people's meat nutrition, meat processing and economic benefits of pig farming. Therefore, the study of pig carcass shape has become an important part of pig breeding projects. Relevant scientific and technological workers from all over the world also regard the growth and development of pig muscles as the focus and focus of research, and eye muscle depth is an important indicator for measuring the growth and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11G16B20/20G16B40/00
CPCC12Q1/6888G16B20/20G16B40/00C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 项韬张云路李新云赵书红梅全顺付川珂
Owner HUAZHONG AGRI UNIV
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