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SD rat-derived mesenchymal stem cell induction medium and induction method

A technology of bone marrow mesenchyme and induction medium, applied in the direction of cell culture active agent, bone/connective tissue cells, culture process, etc. Avoiding Risk and Uncertainty, Efficient Clinical Treatment Research, Simple Ingredient Effect

Active Publication Date: 2021-04-27
北京益华生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the type of factor components after induction is relatively single, only the secretion of GDNF is increased, and it does not increase the secretion of BDNF related to the treatment of ischemic stroke. In addition, the treatment of umbilical cord mesenchymal cells in animal models of ischemic stroke Among them, the effect is not as obvious as that of bone marrow mesenchymal stem cells, and the level of factor secretion induced by its induction scheme is not high, and it cannot be secreted at a high level in a long-term and stable manner.
In addition, low-generation cells are generally used, generally 3-4 generation cells, so the amount of cells that can be induced per unit of primary cells is very limited

Method used

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  • SD rat-derived mesenchymal stem cell induction medium and induction method
  • SD rat-derived mesenchymal stem cell induction medium and induction method
  • SD rat-derived mesenchymal stem cell induction medium and induction method

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Experimental program
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Effect test

Embodiment 1

[0034] A SD rat-derived bone marrow mesenchymal stem cell induction medium, the induction medium is composed of α-MEM medium and additives, in terms of final concentration, the additives are composed of 10ng / ml transforming growth factor-α, 100ng / ml Regulatory protein β1, 1 mM trehalose and 1 mM dibutyryl cyclic adenosine monophosphate composition.

Embodiment 2

[0036] A SD rat-derived bone marrow mesenchymal stem cell induction medium, the induction medium consists of α-MEM medium and additives, in terms of final concentration, the additives are composed of 5ng / ml transforming growth factor-α, 10ng / ml Regulatory protein β1, 100 nM trehalose and 50 nM dibutyryl cyclic adenosine monophosphate composition.

Embodiment 3

[0038] A SD rat-derived bone marrow mesenchymal stem cell induction medium, the induction medium is composed of α-MEM medium and additives, in terms of final concentration, the additives are composed of 100ng / ml transforming growth factor-α, 200ng / ml Composition of regulatory protein β1, 3000nM trehalose and 5000nM dibutyryl cyclic adenosine monophosphate.

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Abstract

The invention discloses an SD rat-derived mesenchymal stem cell induction medium and induction method. The induction medium is composed of an alpha-MEM medium and an additive, wherein the additive is composed of 5-100 ng / ml of a transforming growth factor-alpha, 10-200 ng / ml of regulatory protein [beta]1, 100-3000 nM of trehalose and 50-5000 nM of dibutyryl cyclic adenosine monophosphate according to the final concentration. According to the SD rat-derived mesenchymal stem cell induction culture medium and induction method, the secretion amount of factors such as BDNF, HGF, VEGF and GDNF of rat mesenchymal stem cells can be obviously increased, and the secreted factors are relatively rich in variety and can be stably secreted for a long time; and the risks and uncertainty caused by other methods such as gene modification are avoided through a cytokine induction method, and the induction method is more suitable for industrialization and clinical application.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an induction medium and an induction method for bone marrow mesenchymal stem cells derived from SD rats. Background technique [0002] With the progress of stem cell research, stem cell transplantation has brought a new dawn for the effective treatment of ischemic stroke. Among various types of stem cells, bone marrow mesenchymal stem cells have become an ideal seed cell for transplantation due to their advantages of convenient material extraction, low immunogenicity, and strong expansion ability. Multiple studies have shown that bone marrow mesenchymal stem cell transplantation can improve neurological dysfunction caused by ischemic stroke to varying degrees. Kvortsova et al. transplanted BM-MSCs into the rat model of focal cerebral ischemia through intravenous injection, and found that the transplanted bone marrow mesenchymal stem cells were mainly distributed in the ische...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N2500/34C12N2500/40C12N2501/15C12N2501/998
Inventor 姜粉军仇志强成彦文何子周慧
Owner 北京益华生物科技有限公司
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