A kind of pea peptide with auxiliary hypoglycemic function and preparation method thereof

A pea peptide and blood sugar-lowering technology, which is applied in the field of pea peptide and its preparation, can solve the problems of narrowing the application range of pea protein and affecting the development of pea deep processing, etc., and achieve the effect of controlling blood sugar rise, lowering blood sugar, and wide application prospects

Active Publication Date: 2022-07-01
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, in the production process of pea peptide, pea protein is enzymatically hydrolyzed in order to provide more nutrition for the human body to improve immunity and reduce inflammation, which narrows the application range of pea protein and affects the further development of pea deep processing

Method used

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  • A kind of pea peptide with auxiliary hypoglycemic function and preparation method thereof
  • A kind of pea peptide with auxiliary hypoglycemic function and preparation method thereof
  • A kind of pea peptide with auxiliary hypoglycemic function and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0064] The pea peptide of this embodiment is prepared according to the following method:

[0065] 1. Dissolve 1000g of pea protein powder with a protein content of 80% in 8L of 70°C water and stir to obtain a mixture;

[0066] The mixture was placed in an ultrasonic tank with a constant temperature function, and at 70°C, the ultrasonic tank was turned on and the power was adjusted to 400W for 30min;

[0067] The sonicated mixture was centrifuged at 3000 rpm for 10 min using a desktop centrifuge to collect the solid-phase precipitate;

[0068] Add pure water to the solid-phase precipitation to make the volume to 5L to obtain a slurry;

[0069] 2. After adjusting the pH of the slurry to 8.5 with 30% NaOH solution, under the condition of 50°C insulation, based on the protein content of the pea protein raw material, add alkaline protease at 500U / g, enzymatically hydrolyze for 3 hours, boil at high temperature to inactivate the enzyme, and obtain The first enzymatic hydrolyzate; ...

Embodiment 2

[0092] The pea peptide of this embodiment is prepared according to the following method:

[0093] 1. Dissolve 300kg of pea protein powder with a protein content of 80% in a 2m 3 In a fermenter with water at a temperature of 60°C, stir and set the volume to 3m 3 , to get a mixture;

[0094] The jacket of the fermenter tank is continuously vented with steam to maintain the temperature in the tank at 60-70°C, and the mixture is circulated between the fermenter tank and the pipeline equipped with an ultrasonic vibration plate for 90 minutes with a pump, wherein the area of ​​the ultrasonic vibration plate is 3m 2 , ultrasonic power 45kW (two 0.5m*3m ultrasonic vibration plates are placed opposite each other, the vibration plate efficiency is 1.5w / cm 2 );

[0095] Utilize the horizontal spiral decanter centrifuge to centrifuge the ultrasonically treated mixture at the main motor speed of 3480rpm and the auxiliary motor speed of 2860rpm to collect the solid phase precipitate;

...

Embodiment 3

[0112] 1. Dissolve 500g of pea protein powder with a protein content of 60% in 2.5L of 70°C water, place it in an ultrasonic tank with constant temperature function, stir evenly, heat up to 70°C and keep warm, ultrasonically treat it with a power of 100W, The processing time is 30min;

[0113] Use a desktop centrifuge at 3000 rpm for 10 min, and collect the solid phase precipitate;

[0114] Add pure water to the solid phase precipitation to make the volume to 2.5L to obtain a slurry;

[0115] 2. Adjust the pH of the slurry to 10.0 with 30% NaOH solution, raise the temperature to 50°C, maintain a constant temperature, stir, based on the protein content of the pea protein raw material, add alkaline protease at 800U / g, enzymatically hydrolyze for 3 hours, boil at high temperature to inactivate the enzyme, to obtain the first enzymatic hydrolyzate;

[0116] 3. Centrifuge the first enzymatic hydrolyzate, collect the centrifugation supernatant, and after cooling to room temperatur...

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Abstract

The invention provides a pea peptide with an auxiliary hypoglycemic function and a preparation method and application thereof. The composition of the pea peptide at least includes peptide segments pEE, pEK and pER; based on the quality of the pea peptide, the content of the peptide segment pEE is ≥100.00mg / 100g, the content of peptide pEK is ≥80.00mg / 100g, and the content of peptide pER is ≥90.00mg / 100g. The pea peptide has a more significant effect in reducing blood sugar.

Description

technical field [0001] The invention relates to a pea peptide with an auxiliary hypoglycemic function and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Peas, also known as wheat peas, cold beans, wheat beans, and snow peas, have the characteristics of cold resistance, drought resistance, barren resistance, etc., and are extremely adaptable. Therefore, they are widely distributed in the world, and the planting area and output are relatively stable, with an average annual output. around 10 million tons. The protein content in peas is relatively high, accounting for 23-25% of the mass of dry peas, and the amino acid composition of pea protein is relatively balanced, so pea protein is a high-quality vegetable protein. [0003] At present, the starch and vermicelli industry produces a large amount of pea protein by-products in the production process. These pea protein by-products are simply pea protein after being simply pur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/18A61P33/10
CPCC12P21/06C07K1/36C07K1/34C07K1/18A61P3/10A61P33/10C07K5/06A61K38/00C07K14/415C12Y304/21C12Y304/21004
Inventor 蔡木易谷瑞增陈亮王雨辰刘艳王雨晴王憬张瑞雪徐亚光曹珂璐李国明崔欣悦
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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