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Method for simultaneously detecting five cannabinol compounds by using HPLC-MS/MS

A technology of cannabinol and compounds, applied in the field of simultaneous detection of 5 kinds of cannabinol compounds, to achieve the effect of alleviating difficulties, good recovery rate and easy operation

Pending Publication Date: 2021-04-30
CHINA NAT INST OF STANDARDIZATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there are currently no literature and patent documents that can detect cannabinoids in snack foods

Method used

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  • Method for simultaneously detecting five cannabinol compounds by using HPLC-MS/MS
  • Method for simultaneously detecting five cannabinol compounds by using HPLC-MS/MS
  • Method for simultaneously detecting five cannabinol compounds by using HPLC-MS/MS

Examples

Experimental program
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Effect test

Embodiment 1

[0052] 1) Sample pretreatment:

[0053] Weigh 0.5g of chocolate sample into a 10ml centrifuge tube, cover the lid, preheat in a water bath at 45°C for 5 minutes to melt, then add 2.5ml of methanol, vortex for 2min, centrifuge at 1000rmp for 5min, take the supernatant and pass through 0.22 μm organic filter membrane, ready for use.

[0054] 2) Detection:

[0055] The chromatographic conditions for HPLC separation are: a special column for cannabinol (4.6mm×250I.D., 5.0μm), and the column temperature is 30°C. The mobile phase A was 0.1% formic acid aqueous solution, the mobile phase B was acetonitrile, the flow rate was 1.0 mL / min, and the mobile phase was gradient eluted. The gradient elution program is: 0-15min, 70%-95%B; 15-15.1min, 95%-70%B; 15.1-25min, 70%B.

[0056] 3) Mass Spectrometry:

[0057] The mass spectrometry part adopts the multiple reaction monitoring mode (MRM), and the mass spectrometry parameters of the five cannabinoids are shown in the following table: ...

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Abstract

The invention belongs to the field of analytical chemistry, and particularly relates to a method for simultaneously detecting five cannabinol compounds by using a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS). According to the method, a special chromatographic column for cannabinol is adopted, a formic acid aqueous solution is used as a mobile phase A, acetonitrile is used as a mobile phase B for gradient elution, and a sample enters a detector for detection. The cannabinol compound is tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA), cannabinol (CBN), cannabidiol (CBD) and delta9-tetrahydrocannabinol (THC). The method is high in detection sensitivity, good in recovery rate and easy to operate.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for simultaneously detecting five cannabinol compounds by using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS). Background technique [0002] The main active ingredients in cannabis are cannabinoids, including tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA), cannabinol (CBN), cannabidiol (CBD), △9 -Tetrahydrocannabinol (THC) is the most widely used of these 5 types. [0003] According to literature reports, cannabidiol is currently the most widely used cannabis extract. It is a light yellow resin or crystal, which has obvious therapeutic effects on epilepsy, cancer, multiple sclerosis and other diseases in medicine. △9 - THC has the strongest effect on the central nervous system after inhalation or oral administration, and can interconvert with cannabinol and cannabidiol, so strict control is required. Since ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/7233G01N30/8679G01N2030/146
Inventor 兰韬席兴军初侨于聪聪孟玲玲张文焕张晓芳
Owner CHINA NAT INST OF STANDARDIZATION
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