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Application of vitamin D in preparation of medicine for regulating macrophages

A technology of macrophages and vitamins, applied in the field of biomedicine, can solve problems such as complicated operations, few acquisition channels, and unclear side effects, and achieve the effect of slowing down and improving acute pneumonia

Pending Publication Date: 2021-05-04
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing studies have found that a variety of stimulatory factors (such as the immune regulatory molecule TIPE2, etc.) can act on macrophages to inhibit inflammation, but there are shortcomings such as few acquisition channels, complicated operations, and unclear side effects

Method used

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  • Application of vitamin D in preparation of medicine for regulating macrophages
  • Application of vitamin D in preparation of medicine for regulating macrophages
  • Application of vitamin D in preparation of medicine for regulating macrophages

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The establishment of particulate dust-induced acute pneumonia model and vitamin D intervention model is as follows:

[0043] Healthy 6-8 week old male C57BL / 6 mice were anesthetized by intraperitoneally injecting 0.1 ml / 10 g of 4% chloral hydrate. One week before dust particle treatment, the vitamin D intervention model was established by intraperitoneal injection of 25(OH)D (1000IU / 20g), and the non-exposed trachea was perfused with SiO 2 (3 mg / only) to construct an acute pneumonia model, and recover tissue and alveolar lavage fluid one week later.

Embodiment 2

[0045] Four groups were set up: the control group (Ctrl group), which was healthy C57BL / 6 mice, and non-exposed organs were perfused with 50 μl of normal saline.

[0046] Other experimental groups and construction methods are as follows:

[0047] Dust group (P group; SiO 2 , 3mg / 50μl), which were perfused with SiO through non-exposed trachea in C57BL / 6 mice 2 (3mg / only) constructed acute pneumonia model.

[0048] The vitamin D group [25(OH)D group; 1000IU / 20g] is a vitamin D intervention model constructed by intraperitoneally injecting 25(OH)D (1000IU / 20g) into C57BL / 6 mice.

[0049] Vitamin D intervention group [25(OH)D+P group], it is according to the method for embodiment 1, before giving the dust particle treatment, through intraperitoneal injection 25(OH)D (1000IU / 20g), then through non-exposure Tracheal perfusion with SiO 2 (3mg / only) constructed acute pneumonia model.

[0050] After the experiment, the lung tissues of the mice were collected for H&E staining to clari...

Embodiment 3

[0067] Grouping and model construction refer to Example 2, the difference is that the research object is replaced by Stat6 + / + or Stat6 - / - Mice, 8 mice per group. After one week of feeding, the mice in each group were sacrificed, and the bilateral lung tissues were recovered. The lung tissue was separated into four rings, one quarter was fixed in 4% paraformaldehyde for embedding and sectioning, and the remaining three quarters were frozen and stored in a -80°C refrigerator for later use.

[0068] to Stat6 + / + , Stat6 - / - Mouse lung tissue was stained with H&E, the results are shown in Figure 4 , in which, the black arrow indicates the increase of inflammatory cell infiltration in lung tissue, and the results show that it is related to Stat6 + / + Compared with the mice in the granule group, the infiltration of inflammatory cells in the lung tissue was increased and the alveolar septum was thickened after the mice in the granule group knocked out Stat6; the intervention o...

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Abstract

The invention relates to application of vitamin D in preparation of a medicine for regulating macrophages, discloses a new application of the vitamin D, and provides a new theory and a potential medicine for preventing or clinically treating macrophage-related diseases.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an application of vitamin D in the preparation of medicines for regulating macrophages. Background technique [0002] Vitamin D (Vitamin D) is a fat-soluble vitamin that belongs to cyclopentane polyhydrophenanthrene compounds. Studies have found that vitamin D deficiency is closely related to the occurrence of various diseases (inflammation, asthma, cardiovascular disease, diabetes, hypertension, Parkinson's syndrome, tumors, etc.). Vitamin D closely related to human health includes VD3 (cholecalciferol) and VD2 (ergocalciferol). The main physiological functions of vitamin D and its metabolites are mainly to promote the absorption of calcium and phosphorus in the intestinal tract, inhibit the release of parathyroid glands, maintain normal blood calcium and phosphorus levels, and ensure bone health and normal neuromuscular function. In recent years, the extraskeletal effects of vitami...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/045A61P29/00
CPCA61K31/045A61P29/00
Inventor 陶莎莎杨友静尉淑慧李倩敏
Owner SUZHOU UNIV
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