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A method for preparing metal chelating peptides from poultry feathers

A technology of metal chelating peptides and feathers, applied in the biological field, can solve the problems of high raw material prices, achieve the effect of simple operation, strong practicability, and enhanced activity

Active Publication Date: 2022-01-11
安徽希普生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chinese patent application CN201710732795.9 discloses a fish protein small peptide chelated zinc, including the process of degreasing, enzymatic hydrolysis, and chelation, and provides a method for preparing metal chelating peptides from fish meat, but the price of raw materials is still high , not widely used

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, a kind of method that prepares metal chelating peptide with poultry feather

[0031] A method for preparing metal chelating peptides from poultry feathers, comprising the following steps:

[0032] S1: Pretreatment of feathers: pulverize the duck feathers, place them in an aqueous sodium hydroxide solution with a pH of 7, heat to 85°C, keep the temperature constant for 30 minutes, and simultaneously use microwaves to assist the heating process. The power of the microwaves is 300W, filter, After adding deionized water and mixing, it is spray-dried to obtain pretreated feather meal;

[0033] S2: Segmented enzymatic hydrolysis:

[0034] 1) Place the pretreated feather meal in deionized water, the amount of deionized water is 6 times the mass of the feather meal, to obtain a suspension of feather meal, add mixed enzyme I for enzymolysis, and obtain a primary enzymatic hydrolysis solution, The specific conditions for enzymolysis are: the pH of the enzymolysis ...

Embodiment 2

[0039] Embodiment 2, a kind of method that prepares metal chelating peptide with poultry feather

[0040] A method for preparing metal chelating peptides from poultry feathers, comprising the following steps:

[0041] S1: Pretreatment of feathers: pulverize the duck feathers, place them in an aqueous sodium hydroxide solution with a pH of 9, heat to 100°C, keep the temperature constant for 50 minutes, and simultaneously use microwaves to assist the heating process. The power of the microwaves is 400W, filter, After adding deionized water and mixing, it is spray-dried to obtain pretreated feather meal;

[0042] S2: Segmented enzymatic hydrolysis:

[0043] 1) Place the pretreated feather meal in deionized water, the amount of deionized water is 6 times the mass of the feather meal, to obtain a suspension of feather meal, add mixed enzyme I for enzymolysis, and obtain a primary enzymatic hydrolysis solution, The specific conditions for enzymolysis are: the pH of the enzymolysis...

Embodiment 3

[0048] Embodiment 3, a kind of method that prepares metal chelating peptide with poultry feather

[0049] A method for preparing metal chelating peptides from poultry feathers, comprising the following steps:

[0050] S1: Pretreatment of feathers: pulverize duck feathers, place them in an aqueous sodium hydroxide solution with a pH of 8, heat to 90°C, keep the temperature constant for 40 minutes, and simultaneously use microwaves to assist the heating process. The power of the microwaves is 300W, filter, After adding deionized water and mixing, it is spray-dried to obtain pretreated feather meal;

[0051] S2: Segmented enzymatic hydrolysis:

[0052] 1) Place the pretreated feather meal in deionized water, the amount of deionized water is 6 times the mass of the feather meal, to obtain a suspension of feather meal, add mixed enzyme I for enzymolysis, and obtain a primary enzymatic hydrolysis solution, The specific conditions for enzymolysis are: the pH of the enzymolysis is 7...

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PUM

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Abstract

The invention belongs to the field of biotechnology, and specifically relates to a method for preparing metal chelating peptides from poultry feathers. The method provided by the invention includes: (1) pretreatment of feathers: pulverizing the feathers, placing them in an alkaline solution, heating, and Simultaneously use microwave auxiliary heating process, filter, add deionized water to mix and then spray dry, obtain the feather meal after pretreatment; Enzyme hydrolysis with mixed enzyme Ⅰ, followed by enzymatic hydrolysis with mixed enzyme II containing aminopeptidase and neutral protease, to obtain the enzymatic hydrolysis product of feather meal; (3) metal chelation: the enzymatic hydrolysis product of feather meal React with metal salts to finally produce metal chelating peptides. The metal chelating peptide prepared by the invention has been proved by experiments to have a relatively high chelating rate, and has a wide application range, and provides a new idea for preparing the metal chelating peptide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing metal chelating peptides from poultry feathers. Background technique [0002] my country lacks protein resources, and the protein raw materials of conventional feeds include soybean meal, fish meal, etc., which have been dependent on imports for a long time and have great economic pressure. Therefore, it is urgent to find new protein raw materials with high quality and low price. Feather is one of the important resources in our country, with abundant output, and the annual output can reach one million tons. Feather powder made from various poultry feathers has high protein content. The crude protein content in feathers is more than 80%, and the amino acid content is in More than 70%, the amino acid composition is mainly glycine, serine, cystine and isoleucine, which have the essential amino acids needed by many animals, but the molecular shape is si...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/113
CPCC12P21/06C07K1/113
Inventor 王俊青赵大伟王伦学余忠丽
Owner 安徽希普生物科技有限公司