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Breeding method and application of desulfurization strain with high sulfur oxidation performance

A technology of desulfurization bacteria and sulfur oxidation, applied in the field of bioengineering, can solve the problems of few desulfurization strains and few desulfurization strains, etc., and achieve the effects of increasing the production rate of elemental sulfur, lowering the operating cost, and improving the sulfur oxidation performance.

Active Publication Date: 2021-05-11
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, there are still relatively few desulfurization strains with high sulfur oxidation performance in the prior art, and there are few strategies on how to breed desulfurization strains with high sulfur oxidation performance.

Method used

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  • Breeding method and application of desulfurization strain with high sulfur oxidation performance
  • Breeding method and application of desulfurization strain with high sulfur oxidation performance
  • Breeding method and application of desulfurization strain with high sulfur oxidation performance

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Embodiment 1

[0048]This embodiment provides a breeding method of high-sulfur oxidative detholite strains, as follows:

[0049](1) Inoculated into the TD medium to TD medium, the wild desirabus d301 was cultured in 180 rpm and 30 ° C for 30 hours, and the sulfur particles were removed by low speed at 1000 rpm, 2 min, and then transferred to a new centrifuge, The cells were collected from centrifugation at 8000 rpm and 5 min, and then suspended with a clean TD medium, wash cells, centrifugally collected. Suspended by TD medium, dimming density OD600 is 1.0, diluted to cell density of 105CFU / ml.

[0050](2) Take the above 0.1 ml of diluted bacterial liquid coated on TDS solid medium containing 120 mmol / L of sulfide, plasma treatment. The position of the electrode is adjusted such that the distance between the upper and lower electrodes is controlled at 3 mm, the voltage 3V and current 0.5a are adjusted, and the gas generates discharge to obtain a uniform air medium blocking discharge plasma. Sustaine...

Embodiment 2

[0057]The present embodiment performs biosulfurization performance verification of the high-sulfur oxidative detholane strain D309 screened by wild-type strain D301 and Example 1.

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Abstract

The invention relates to a breeding method and application of a desulfurization strain with high sulfur oxidation performance, the breeding method is based on a plasma treatment technology, high sulfide conditions are taken as screening pressure, a plurality of complete bacterial colonies are selected by using a plate with gradient sulfide concentration, the change of the sulfur oxidation performance of each strain is investigated through culture of high-concentration sulfide, the sulfur oxidation performance of the cells is determined through a respiration determination method, and finally a target strain is screened out. According to the breeding method, the strain with good desulfurization performance and high treatment efficiency can be bred, and compared with a wild strain, the sulfur oxidation performance of the bred strain is improved, sulfate radical accumulation is remarkably reduced, and the elemental sulfur generation rate is increased, so that the system is low in energy consumption and low in operation cost, deep popularization of biological desulfurization is powerfully promoted, the robustness of biological desulfurization is enhanced, and a very valuable thought is provided for the biological desulfurization technology to deal with the problem of complex and changeable gas components in practical application.

Description

Technical field[0001]The present invention belongs to the field of bio-engineering technology, and is specifically involved in the breeding method of high sulfur oxidative detholicin strains and their applications.Background technique[0002]Biological desulfurization is a technique that removes sulfide in water using a microorganism having sulfur oxidation properties at normal temperature. Using this method, you can use the h in the h in biogas.2S removed. The desulfurization capacity of the biological desulfurization process tends to be directly related to the desulfurization performance of the sulfur oxidation microorganism. In addition, in the actual situation, the change in production conditions changes vulnerability in the gas, once in the case of hydrogen sulfide, It may cause a certain impact on the desulfurization system, affecting the production of production. Therefore, high-performance strains can effectively reduce the risk of accidents, while also improving the stability...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12R1/01
CPCC12N1/20C12N1/02Y02A50/20
Inventor 穆廷桢刑建民杨茂华苗得露赵胥浩
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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