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Solid grain leavening agent and preparation method thereof

A starter and grain technology, applied in the field of grain solid starter and its preparation, can solve the problem of high cost of liquid fermentation, achieve the effects of increasing market and application value, reducing pollution, and low cost

Inactive Publication Date: 2021-05-14
YULIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a grain solid starter and its preparation method to solve the current technical problem of high cost of liquid fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of preparation method of cereal solid starter in the embodiment of the present invention, comprises the following steps:

[0026] Step 1: Screening of grains: select grain kernels with full grains, remove impurities, shriveled grains, and bad grains, wash and dry;

[0027] Step 2: Sterilize the grains after boiling and ripening: add water to the grains for boiling, the cooking time is 1 hour, and sterilize with moist heat steam;

[0028] Step 3: Inoculate the fermented bacteria into the lactic acid bacteria culture medium, and activate it by static culture at 30°C for 50 hours. Use a centrifuge to collect the bacteria at 7000 rpm at 8°C and wash to prepare a concentration of 120cfu / ml of starter to obtain the prepared starter;

[0029] Step 4: Inoculation and fermentation: Spray the prepared starter into the surface of the ripened and sterilized grain particles, the inoculum amount is 0.5%, and use stirring equipment to mix the starter so that it is evenly dist...

Embodiment 2

[0032] A kind of preparation method of cereal solid starter in the embodiment of the present invention, comprises the following steps:

[0033] Step 1: Screening of grains: select grain kernels with full grains, remove impurities, shriveled grains, and bad grains, wash and dry;

[0034] Step 2: Sterilize the grains after cooking and ripening: add water to the grains for boiling, the cooking time is 2 hours, and sterilize with moist heat steam;

[0035] Step 3: Inoculate the fermented bacteria into the lactic acid bacteria culture medium, and activate it by static culture at 33°C for 40 hours. Use a centrifuge to collect the bacteria at 7000 rpm at 8°C, wash them, and prepare a concentration of 130cfu / ml of starter to obtain the prepared starter;

[0036] Step 4: Inoculation and fermentation: Spray the prepared starter into the surface of the ripened and sterilized grain particles, the inoculum amount is 0.5%, and use stirring equipment to mix the starter so that it is evenly...

Embodiment 3

[0039] A kind of preparation method of cereal solid starter in the embodiment of the present invention, comprises the following steps:

[0040] Step 1: Screening of grains: select grain kernels with full grains, remove impurities, shriveled grains, and bad grains, wash and dry;

[0041] Step 2: Sterilize the grains after boiling and ripening: add water to the grains for cooking, the cooking time is 1.5 hours, and sterilize with moist heat steam;

[0042] Step 3: Inoculate the fermented bacteria into the lactic acid bacteria culture medium, and incubate at 35°C for 25 hours for activation. Use a centrifuge to collect the bacteria at 7000 rpm at 8°C, wash them, and prepare a concentration of 111cfu / ml of starter to obtain the prepared starter;

[0043] Step 4: Inoculation and fermentation: Spray the prepared starter into the surface of the ripened and sterilized grain particles, the inoculum amount is 0.5%, and use stirring equipment to mix the starter so that it is evenly dis...

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PUM

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Abstract

The invention discloses a grain solid-state leavening agent and a preparation method thereof. The preparation method comprises the following steps: step 1, selecting grains according to a preset standard and drying the grains; step 2, disinfecting and sterilizing the grains dried in the step 1; step 3, inoculating a fermentation strain into a lactic acid bacteria culture medium, standing and culturing for 25-50 hours at the temperature of 30-35 DEG C, activating, centrifugally collecting thalli by using a centrifugal machine at the speed of 7000 revolutions per minute at the temperature of 8 DEG C, and washing, so as to prepare a leavening agent with a preset concentration, so as to obtain the prepared leavening agent; and step 4, spraying the prepared leavening agent obtained in the step 3 into the surfaces of the cereal particles obtained in the step 2, uniformly mixing and fermenting by using stirring equipment, so that the leavening agent is uniformly distributed on the surfaces of the cereal particles, keeping the wettability of the surfaces of the cereal particles to obtain original grain fermented cereal, and freeze-drying to obtain the cereal solid leavening agent. The preparation method takes cereals as a culture medium, so that the fermentation cost is low. The special appliances are not needed, so that the device can be realized by general families and is suitable for low-tech areas.

Description

technical field [0001] The invention belongs to the technical field of grain fermentation, and in particular relates to a grain solid starter and a preparation method thereof. Background technique [0002] In the current field of grain fermentation, the grain is mainly fermented in a liquid fermentation mode. Liquid fermentation technology is one of the modern biotechnologies. It refers to imitating nature in biochemical reactors to make sugars, organic and inorganic nitrogen-containing compounds, inorganic salts and other trace elements necessary for the growth of strains. Nutrients are dissolved in water as a medium, after sterilization, the bacteria are inserted, sterile air is introduced and stirred to provide oxygen suitable for the respiration and metabolism of the bacteria, and control the appropriate external conditions to carry out mass culture and reproduction of bacteria the process of. Industrialized large-scale fermentation culture is fermentation production, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L7/104A23L33/135C12N1/14C12N1/20C12R1/845
CPCA23V2002/00A23L7/104A23L33/135C12N1/14C12N1/20A23V2200/30
Inventor 王富刚陈花艾银婷王建武
Owner YULIN UNIV
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