Combined vaccine for preventing hand-foot-and-mouth disease as well as preparation method and application thereof

A combined vaccine and hand-foot-mouth disease technology, applied in the biological field, can solve the problem of weak cross-protection of Coxsackie virus, and achieve the effects of simplified vaccination procedures, good stability, good immunogenicity and safety

Active Publication Date: 2021-05-14
SINOVAC BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the EV71 vaccine has been released. According to clinical research data, the use of the vaccine can prevent hand, foot and mouth disease caused by EV71, especially for severe cases. Significantly, but the cross-protection of EV71 vaccine against Coxsackie virus is weak. In view of the trend of enterovirus serotype changes, there is an urgent need for a multi-serotype combination vaccine that can prevent hand, foot and mouth disease in a wider range

Method used

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  • Combined vaccine for preventing hand-foot-and-mouth disease as well as preparation method and application thereof
  • Combined vaccine for preventing hand-foot-and-mouth disease as well as preparation method and application thereof
  • Combined vaccine for preventing hand-foot-and-mouth disease as well as preparation method and application thereof

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Embodiment Construction

[0029] The present invention will be further described by the following examples. It should be understood that the examples of the present invention are only used to illustrate the present invention, rather than limitation of the present invention. Simple improvements to the present invention under the concept of the present invention all belong to the present invention Scope of protection claimed.

[0030] 1. Preparation of finished combination vaccine

[0031] (1) Isolation of strains

[0032] Pharyngeal swabs or feces of patients with hand, foot and mouth disease were obtained from various centers for disease control and prevention, diluted with saline, centrifuged, and sterilized by filtering with 0.45 μm and 0.22 μm filters. Select sensitive cells (Vero cells or human diploid cells) that are well grown and confluent to a monolayer. The virus culture solution is 199 solution containing 2% calf serum, inoculate the processed throat swab / feces specimen according to a certa...

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Abstract

The invention discloses a combined vaccine for preventing a hand-foot-and-mouth disease. The combined vaccine comprises an inactivated enterovirus 71 type virus and an inactivated coxsackie virus A group 10 type virus. The invention also discloses a method for preparing the combined vaccine. The prepared vaccine has good adsorption effect and stability. Multiple antigens involved in the invention do not interfere with each other in antigenicity and immune effect after immunizing an object, and have good immunogenicity and safety. The use of the combined vaccine can obviously simplify a vaccination procedure, improve vaccination efficiency and reduce cost.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a combined vaccine for preventing hand-foot-mouth disease and its preparation method and application. Background technique [0002] Hand-foot-mouth disease (HFMD) is listed as a Class C infectious disease in my country's "Infectious Disease Prevention and Control Law". It is an acute infectious disease caused by a variety of enteroviruses, and it is more common in infants and young children. . It is prevalent in summer, with a high incidence in preschool children and adults as indirect sources of infection. It mainly manifests as rashes on the mouth, hands, and feet, and can be complicated by meningitis, encephalitis, pulmonary edema, and circulatory failure. The viruses that cause HFMD include enterovirus 71 (Enterovirus 71, EV71) and CA16 (Coxasckievirus A16, CA16), which are more common, and also include Coxsackievirus A (Coxasckievirus A, CA) 2, 4, Types 5, 6, 10, et...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K39/13A61K39/125A61K39/39A61P31/14
CPCA61K39/12A61K39/39A61P31/14A61K2039/70A61K2039/5252C12N2770/32334C12N2770/32034A61K2039/55505
Inventor 刘旭洋高虹单慈恩鲍凯凯朱朗高强尹卫东
Owner SINOVAC BIOTECH
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