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Chiral chromatographic separation analysis method of dihydroquercetin enantiomer

A dihydroquercetin, separation and analysis technology, applied in material separation, analysis materials, measuring devices, etc., can solve problems such as differences

Active Publication Date: 2021-05-14
广州研创生物技术发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the efficacy of two enantiomers of some chiral drug molecules is very different, or even completely opposite.

Method used

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  • Chiral chromatographic separation analysis method of dihydroquercetin enantiomer
  • Chiral chromatographic separation analysis method of dihydroquercetin enantiomer
  • Chiral chromatographic separation analysis method of dihydroquercetin enantiomer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of chiral chromatographic separation analysis method of dihydroquercetin enantiomer is as follows:

[0027] (1) Instruments and conditions: High performance liquid chromatography: Agilent 1200 series liquid chromatography

[0028] Chromatographic column: the specification is 250×4.6mm; the column is filled with cellulose-tris(4-methylbenzoate) chiral stationary phase produced by Guangzhou Yanchuang Biotechnology Development Co., Ltd., namely Y2.

[0029] Mobile phase: n-hexane: trifluoroacetic acid: ethanol = 80:0.1:20 (V / V / V)

[0030] Flow rate: 1.0mL / min

[0031] Column temperature: 25°C

[0032] Injection volume: 10μL

[0033] Detection wavelength: 290mm

[0034] (2) Implementation

[0035] Dihydroquercetin (purity is 99%) is formulated into 0.5mg / mL solution with ethanol, on Agilent 1200 series high-performance liquid chromatograph, adopt Y2 chromatographic column (250×4.6mm), the mobile phase is n-hexane:trifluoroacetic acid:ethanol=80:0.1:20 (V / V / V...

example 2

[0037] A kind of chiral chromatographic separation analysis method of dihydroquercetin enantiomer is as follows:

[0038] (1) Instruments and conditions: High performance liquid chromatography: Agilent 1200 series liquid chromatography

[0039] Chromatographic column: the specification is 250×4.6mm; the column is filled with amylose (3,5-dimethylphenylcarbamate) chiral stationary phase produced by Guangzhou Yanchuang Biotechnology Development Co., Ltd., namely Y3.

[0040] Mobile phase: n-hexane: trifluoroacetic acid: ethanol = 80:0.1:20 (V / V / V)

[0041] Flow rate: 1.0mL / min

[0042] Column temperature: 25°C

[0043] Injection volume: 10μL

[0044] Detection wavelength: 290mm

[0045] (2) Implementation

[0046] Dihydroquercetin (purity is 99%) is formulated into 0.5mg / mL solution with ethanol, on Agilent 1200 series high-performance liquid chromatograph, adopt Y3 chromatographic column (250×4.6mm), the mobile phase is hexane:trifluoroacetic acid:ethanol=80:0.1:20 (V / ...

example 3

[0048]A kind of chiral chromatographic separation analysis method of dihydroquercetin enantiomer is as follows:

[0049] (1) Instruments and conditions: High performance liquid chromatography: Agilent 1200 series liquid chromatography

[0050] Chromatographic column: the specification is 250×4.6mm; the column is filled with cellulose-tris(3-chloro-4-methylphenylcarbamate) chiral stationary phase produced by Guangzhou Yanchuang Biotechnology Development Co., Ltd., namely Y5.

[0051] Mobile phase: n-hexane: trifluoroacetic acid: ethanol = 80:0.1:20 (V / V / V)

[0052] Flow rate: 1.0mL / min

[0053] Column temperature: 25°C

[0054] Injection volume: 10μL

[0055] Detection wavelength: 290mm

[0056] (2) Implementation

[0057] Dihydroquercetin (purity is 99%) is formulated into 0.5mg / mL solution with ethanol, on Agilent 1200 series high-performance liquid chromatograph, adopt Y5 chromatographic column (250×4.6mm), the mobile phase is n-hexane: trifluoroacetic acid: ethanol...

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Abstract

The invention discloses a chiral chromatographic separation and analysis method of a dihydroquercetin enantiomer. The method comprises the following steps of dissolving a dihydroquercetin sample in ethanol, carrying out gradient elution by adopting a chiral chromatographic column and taking n-hexane, trifluoroacetic acid and lower alcohol as mobile phases, and carrying out liquid chromatography separation analysis by adopting a chiral stationary phase. The invention belongs to the technical field of chiral chromatographic separation and particularly relates to a method for preparing a racemic dihydroquercetin enantiomer, which can discover that the racemic dihydroquercetin enantiomer realizes good chiral separation by combining a mobile phase on a coating type and bonding type polysaccharide derivative chiral stationary phase material, and is high in analysis and separation speed and high in resolution; the racemic dihydroquercetin can be analyzed, detected and put into a separation analysis method.

Description

technical field [0001] The invention belongs to the technical field of chiral chromatographic separation, in particular to a chiral chromatographic separation and analysis method of dihydroquercetin enantiomers. Background technique [0002] With the development of stereochemistry, the study of stereoselectivity of chiral drugs has attracted much attention. Chiral isomers with the same physical and chemical properties may exhibit completely different absorption, metabolism, pharmacological effects, teratogenic, carcinogenic, mutagenic and endocrine disrupting activities in vivo. For example, the efficacy of two enantiomers of some chiral drug molecules is very different, or even completely opposite. A typical example is that of the two isomers of thalidomide, only the (R)-enantiomer has a sedative effect, while the (S)-enantiomer is a powerful teratogen that can cause fetal malformations. Another example is that the antibacterial activity of levofloxacin is 8 to 12 times t...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/26
CPCG01N30/02G01N30/26
Inventor 毛志平许文业郭栋梁绍武王子杏
Owner 广州研创生物技术发展有限公司
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