The invention discloses a synthetic method of 20,23-dipiperidine-5-O-carbomycin amine glycosyl-tildipirosin. The synthetic method comprises the following steps: (1) adding 20,23- dihalo-5-O-carbomycinamine glycosyl-tildipirosin, piperidine and potassium carbonate in a reaction container, carrying out heating refluxing for 4-5 h while stirring, after fluxing is finished, filtering an obtained mixing system, adding purified water in filtrate, fully stirring and then standing for layering, and separating to obtain an organic layer; (2) adding pure water in the organic layer again, adjusting pH value to be 2-3, standing for layering, separating to obtain a water layer, cooling the water layer to the temperature of 0 DEG C, adjusting pH value to be 8.0-8.5, standing for layering, separating toobtain a water phase, extracting the water phase by using an organic solvent to obtain an organic phase, removing the organic solvent, filtering, and concentrating till the product is dry to obtain acrude product; and (3) refining the crude product to obtain the 20,23-dipiperidyl-5-O-carbomycin amine glycosyl-tildipirosin. By the method, the 20,23- dihalo-5-O-carbomycin amine glycosyl-tildipirosin is directly used as a starting material, the route for synthesizing tildipirosin is greatly simplified, and the synthesizing cost is reduced.