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Digital detection method and system for predicting drug resistance of transgenic maize

A technology of genetically modified corn and digital detection, applied in radio wave measurement systems, neural learning methods, measurement devices, etc., can solve the problems of short test cycle, time-consuming and laborious, not completely consistent, and achieve the effect of rapid screening and performance evaluation

Active Publication Date: 2021-05-25
ZHEJIANG UNIV
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Problems solved by technology

Among them, the determination of morphological characteristic parameters such as plant height, leaf length, and leaf width is mostly based on manual measurement, which is time-consuming, laborious, long detection cycle, and low efficiency.
The invention patent with publication number CN106576829A discloses a method for identifying glyphosate-resistant corn varieties. The method takes corn seeds as objects, and determines whether the test seeds are resistant to glyphosate by testing the changes in physiological and morphological indicators of corn seeds. Although this method has low requirements on the surrounding environment and a short test period, due to the complex and changeable environment in the growth process of corn, the final performance of pesticide resistance is not completely consistent with that of seeds.

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  • Digital detection method and system for predicting drug resistance of transgenic maize
  • Digital detection method and system for predicting drug resistance of transgenic maize
  • Digital detection method and system for predicting drug resistance of transgenic maize

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Experimental program
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Embodiment 2

[0099] Such as figure 2 As shown, a digital detection system for predicting drug resistance of transgenic corn provided in this embodiment includes:

[0100] The detection information acquisition module 201 is used to obtain the detection information of the corn plants sprayed with the chemical agent at the current moment; the detection information includes RGB images, three-dimensional point cloud data and relative content of chlorophyll.

[0101] The pixel ratio calculation module 202 is used to calculate the pixel ratio of the corn plant at the current moment according to the RGB image at the current moment; the pixel ratio is the number of pixels in the first area of ​​the corn plant and the second area of ​​the corn plant The ratio of the number of pixels in the area; the first area of ​​the corn plant is the area of ​​the leaves of the corn plant that changes after being exposed to the chemical agent, and the second area is the area of ​​all the leaves of the corn plant...

Embodiment 3

[0121] The digital detection method for predicting the drug resistance of transgenic corn provided by this embodiment comprises the following steps:

[0122] Step S1: Preparation of experimental materials.

[0123] Several transgenic corn seeds (glyphosate-insensitive5-enolpyruvylshikimate-3-phosphate synthase, EPSPS) used in this embodiment and non-transgenic seeds are provided by Zhejiang Academy of Agricultural Sciences, glyphosate isopropylamine saline solution (400ml) is provided by Hangzhou City, Zhejiang Province Provided by Plant Protection Station. Before sowing, the above-mentioned seeds need to be soaked in clear water for 12 hours to make them fully absorb water and facilitate germination.

[0124] Step S2: Corn sample preparation.

[0125] The soaked seeds are sown in flower pots (upper diameter 11.5cm, height 11cm, bottom diameter 8cm), in order to ensure that each flower pot has a strain of corn, two corn seeds need to be sown in each flower pot. After labeli...

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Abstract

The invention discloses a digital detection method and system for predicting drug resistance of transgenic maize, and relates to the technical field of maize variety drug resistance detection.The digital detection method comprises the steps of acquiring RGB images, three-dimensional point cloud data and chlorophyll relative content of maize plants after drug spraying at the current moment; calculating a pixel ratio and morphological characteristics according to the RGB image and the three-dimensional point cloud data; inputting the detection parameters of the corn plant at the current moment into the series model to predict the detection parameters at the next moment, further obtaining a detection parameter change curve chart of the corn plant at the next time period, and determining the drug resistance characteristic of the corn plant according to the detection parameter change curve chart; wherein the detection parameters comprise chlorophyll relative content, pixel ratio and morphological characteristics; and inputting the detection parameters of the corn plant at the current moment into the parallel model to predict the variety of the corn plant. According to the invention, rapid screening and performance evaluation of the drug-resistant transgenic maize variety can be realized.

Description

technical field [0001] The invention relates to the technical field of detection of drug resistance of corn varieties, in particular to a digital detection method and system for predicting the drug resistance of transgenic corn. Background technique [0002] In today's increasingly serious food security problem, corn, as one of the important food crops in our country, its output is related to the stability of the society, the prosperity of the economy and the strength of the country's comprehensive national strength. The harvest of maize is not only related to the genotype, but also affected by the complex environment. In other words, the excellent genotype of maize can be fully expressed only under suitable environmental conditions. Among them, weeds in the corn growth environment have tenacious vitality and often compete with corn for limited resources such as light, water, nutrients, and inorganic salts. [0003] Therefore, herbicides become a necessity in agricultural p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T7/00G06T7/60G06T7/90G06Q50/02G06N3/04G06N3/08
CPCG06T7/0012G06T7/90G06T7/60G06Q50/02G06N3/08G06T2207/10024G06T2207/20081G06T2207/20084G06T2207/30188G06N3/044G06N3/045A01M7/0089G01S17/42G01S17/88G01S7/4808G06T7/0016G06T2207/10016A01G22/20G06V10/82G06V20/68G06N3/084G06N20/10G06T7/136G06T7/62G06T7/11G01S17/894G06T17/00G06T2207/10028G06T2207/20072G06T2207/30128
Inventor 冯旭萍陶明珠何勇杨睿张金诺史永强
Owner ZHEJIANG UNIV
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