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Acid-resistant streptomyces albidus and application thereof in fermentation of epsilon-polylysine

A Streptomyces albicans, inoculation fermentation technology, applied in the direction of fermentation, bacteria, microorganism-based methods, etc., can solve problems such as stagnation of bacterial growth products

Active Publication Date: 2021-05-28
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using this pH control method, the yield of ε-polylysine is increased by more than 50% compared with the common two-stage pH control process; Synthesis is stagnant, even if the pH is raised to 4.5 after the stress ends, it will take a long time for the bacteria to rejuvenate

Method used

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  • Acid-resistant streptomyces albidus and application thereof in fermentation of epsilon-polylysine
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  • Acid-resistant streptomyces albidus and application thereof in fermentation of epsilon-polylysine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Breeding method of Streptomyces parvus AAE89

[0044] Taking the wild Streptomyces parvus QLU58 screened in the campus soil of Qilu University of Technology Changqing Campus as the starting strain, it was cultured in the M3G liquid medium with pH 6.8 at 30°C and 200 rpm to the logarithmic growth phase, and the mass percentage was expressed as 10% of the inoculum was inoculated in the M3G liquid medium of pH 4.0, fermented and cultivated at 30°C 200rpm for 4 days (the pH dropped to about 3.1 after 1 day of fermentation, and then remained unchanged), and then the fermentation broth was calculated by mass percentage Re-transfer to fresh pH4.0 M3G liquid medium at a ratio of 10% for fermentation and culture for 4 days. After 10 transfers, reduce the initial pH of the M3G liquid medium to 3.9, and gradually reduce the pH of the M3G liquid culture through repeated operations. During this process, the ε-PL high-producing bacteria with improved acid resistance were regularly sc...

Embodiment 2

[0050] The growth characteristics of Streptomyces albus QLU58 and Streptomyces albus AAE89 under acid stress conditions, the specific experimental methods are as follows:

[0051] (1) The spores of the original bacteria Streptomyces albicans QLU58 and the selected acid-resistant bacteria Streptomyces albicans AAE89 were respectively inoculated into M3G liquid medium (the initial concentration of spores in the liquid medium after inoculation was 1×10 5 ~1×10 8 cells / mL), cultured at 30°C for 24 h on a 200 rpm shaker with ventilation and shaking as the seed solution.

[0052] (2) Inoculate the seed liquids of Streptomyces albicans QLU58 and Streptomyces albicans AAE89 into the M3G liquid medium of pH 4.0 with 4% inoculum amount in terms of mass percentage, and ferment at 30° C. with 200 rpm shaker ventilation and vibration On 2d, samples were taken every 12 hours in the middle, and the dry weight and pH of the bacteria were measured; as the fermentation process progressed, the ...

Embodiment 3

[0054] The acid tolerance experiment of Streptomyces albicans QLU58 and Streptomyces albicans AAE89, the specific experimental method is as follows:

[0055] (1) The spores of the original bacteria Streptomyces albicans QLU58 and the selected acid-resistant bacteria Streptomyces albicans AAE89 were respectively inoculated into M3G liquid medium (the initial concentration of spores in the liquid medium after inoculation was 1×10 5 ~1×10 8 cells / mL), cultured at 30°C for 24 h on a 200 rpm shaker with ventilation and shaking as the seed solution.

[0056] (2) Inoculate the seed liquids of Streptomyces albicans QLU58 and Streptomyces albicans AAE89 into the M3G liquid medium of pH 3.0 with 4% inoculum amount in terms of mass percentage, and cultivate them in a ventilated shaking table at 30°C and 200rpm After 48 hours, samples were taken every 12 hours in the middle, the diluted bacterial solution was spread on a plate, the number of viable bacteria was measured, and the survival...

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Abstract

The invention relates to a strain of streptomyces albidus AAE89, which is named as Streptomyces albidus AAE89 in Latin. The strain is preserved in China General Microbiological Culture Collection Center on October 12, 2020, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC 20880; the streptomyces albidosus AAE89 provided by the invention has stronger acid resistance, and compared with an original strain, the streptomyces albidosus AAE89 has the advantages that the dry weight of a thallus is increased by 46.92% after the streptomyces albidosus AAE89 is cultured for 48 hours under the condition that the initial pH is 4.0, and the survival rate of the thallus is increased by 1.81 times after the thallus is stressed for 48 hours under the condition that the pH is 3.0.

Description

technical field [0001] The invention belongs to the field of microbial fermentation engineering, and in particular relates to an acid-resistant Streptomyces parvus and its application in the fermentation of ε-polylysine. Background technique [0002] ε-polylysine (ε-PL) is a homotype amino acid polymer that is composed of 25-35 L-lysines connected by heteromorphic peptide bonds formed by α-carboxyl and ε-amino groups. ε-PL has a wide antibacterial spectrum, good water solubility, high safety, and good thermal stability. It is widely used as a food preservative in Japan, South Korea, the United States and other countries; my country also officially approved it as a new food additive in 2014. Variety. At the same time, as a cationic biopolymer, ε-PL is widely used as biodegradable materials, drug carriers, biochip coatings, emulsifiers, high-absorbency hydrogels and anticancer enhancers, etc. market application prospects. [0003] At present, industrialized ε-PL is mainly pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/02C12R1/465
CPCC12P13/02
Inventor 任喜东董一娴宋菁王亦平杜超凡刘新利
Owner QILU UNIV OF TECH
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