Peripheral blood-based LCL-NK cell co-culture method, cell and product

A combined culture and peripheral blood technology, applied in the direction of cell culture active agents, blood/immune system cells, animal cells, etc., can solve the problems of unsatisfactory clinical effect, insufficient NK cell number and killing activity, etc.
CN112852728AActive Publication Date: 2021-05-28JIANGSU MOBILI BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU MOBILI BIOTECH CO LTD
Publication Date
2021-05-28

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Abstract

The invention provides a peripheral blood-based LCL-NK cell co-culture method, whichcomprises the following steps: preparing and obtaining the PBMC from the peripheral blood, and preparing the LCL cells from the PBMC, wherein the LCL cells are obtained by culturing PBMC (peripheral blood mononuclear cells) in supernate containing EBV (electron barr virus); the EBV supernate is obtained by carrying out culture passage on B958 cells, then carrying out starvation lysis and then separating; and co-culturing the cells in a culture system formed by PBMC, LCL cells, cell factors and a culture solution. According to the culture method provided by the scheme, the purity of a cell product is effectively improved, and the culture efficiency, the NK cell killing property and the like are remarkably improved.
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Description

technical field

[0001] The invention belongs to the technical field of biology and biomedicine, and in particular relates to a peripheral blood-based LCL-NK cell joint culture method, cells and products. Background technique

[0002] The immune response protects the body from pathogens, and the immune system is composed of numerous immune-related cells and cytokines. White blood cells, especially lymphocytes, play an important role in the immune system. Representative cells constituting lymphocytes include cells of the innate immune system and cells of the acquired immune system. Natural killer cells (NK cells) are a representative innate immune cell. It is known that natural killer cells can kill tumors in a non-specific way, recognize and kill viruses, bacteria, etc., and can kill with enzymes such as perforin and granzyme Pathogens, or kill pathogens through Fas-FasL interaction. According to reports, for tumor patients, the reduction of the ability of NK cells to kill...

Claims

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