Multiple nucleic acid amplification product detection method and kit

Abstract

The invention belongs to the technical field of acid detection, and discloses a multiple nucleic acid amplification product detection method, which comprises: establishing an amplification reaction system containing a display agent, the amplification reaction system further comprising a sample to be detected, a primer and an amplification reagent; then interpreting an amplification result of the amplification reaction system through a chromogenic dye method; and judging whether test strip detection is carried out or not according to the interpreted amplification result. According to the method, a color developing dye detection method and test strip detection are combined, a color developing agent and different modified primers are added into a multiple nucleic acid amplification system, firstly, a nucleic acid amplification result is visually interpreted by utilizing a color developing reaction of the color developing agent, and whether a to-be-detected solution is successfully amplified or not is judged; and the detection of the target nucleic acid in the successfully amplified to-be-detected solution is completed through an immunochromatography test strip. Therefore, false positive samples in the positive samples can be removed, the false positive rate is reduced, the detection accuracy is improved, meanwhile, all the detection lines of the test strip can be used for detecting indexes, and the multiple detection capacity of an amplification system is guaranteed.

Description

technical field [0001] The invention relates to the technical field of nucleic acid detection, in particular to a detection method and a kit for multiple nucleic acid amplification products. Background technique [0002] The diagnosis of infectious diseases caused by pathogen infection often needs to be confirmed in combination with etiological examination. Multi-infection) pathogen detection often requires the use of multi-target joint detection. [0003] In the new coronavirus nucleic acid detection primers and probe sequences released by the Chinese Center for Disease Control and Prevention of Viral Diseases, it is stated that the target sequences of the new coronavirus detection kit are open reading frame 1ab (open reading frame, ORF1ab), nucleocapsid Protein (nucleoprotein, N) gene region; at the same time, the human gene should be used as an internal reference to monitor the effect of sample collection and extraction to avoid false negative results. However, using si...

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Problems solved by technology

[0004] At present, the nucleic acid detection of multi-target pathogens in the market mainly uses multiplex PCR to achieve amplification. The corresponding detection techniques such as PCR probe method, multiplex amplification-hybridization, multiplex amplification-capillary electrophoresis, and multiplex amplification-mass spectrometry are only applicable to large hospitals. Large-scale medical institutions such as epidemic prevention and control centers are difficult to use for rapid on-site inspection at the grassroots level and early screening of pathogen epidemics due to high equipment costs, high operating environment, and high personnel requirements.

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