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Cysteine protease inhibitor B detection device as well as preparation method and application thereof

A cysteine ​​protease and detection device technology, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of inability to reduce sensitive concentration, limited concentration detection range and detection variation range, etc.

Active Publication Date: 2021-05-28
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Description
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Problems solved by technology

[0004] The present invention aims at the technical problem that the existing CSTB itself saliva detection method has limited concentration detection range and detection change interval, and cannot detect the decrease of its concentration sensitively and specifically, and provides a method that can detect the decrease of CSTB concentration sensitively and specifically. Cysteine ​​protease inhibitor B detection device and its preparation method and application

Method used

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  • Cysteine protease inhibitor B detection device as well as preparation method and application thereof
  • Cysteine protease inhibitor B detection device as well as preparation method and application thereof
  • Cysteine protease inhibitor B detection device as well as preparation method and application thereof

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Embodiment 1

[0021] (1) Construction of asymmetric nanopores: Experiments were carried out using polyethylene terephthalate (PET) membranes that formed bombardment tracks after bombardment with heavy ions to construct an asymmetric nanopore system. The membrane thickness is 12 μm, and the track after bombardment is the full length of the asymmetric nanopore. The front and back sides of the film were irradiated with ultraviolet lamp for 1 hour each. Then perform asymmetric optical nanopore etching on the membrane: preheat the etching device, etching solution (9M sodium hydroxide solution) and stop solution (1M formic acid + 1M potassium chloride) on a hot stage at 30°C for 30 minutes, And the operation is carried out on the hot stage. The membrane was clamped in the etching device, and the real-time change of the current on both sides of the membrane was monitored by a picoammeter at a voltage of 1V. An etching solution is added to the positive side of the membrane, and a blocking solutio...

Embodiment 2

[0026] Take the saliva in the mouth of the patient more than 2 hours after eating: instruct the patient to rinse the mouth with normal saline and take 2ml of saliva, first centrifuge at 1500rcf, 4℃ for 5 minutes to obtain the supernatant; then use the supernatant at 15000rcf, 4℃ The supernatant obtained by centrifugation for 5 minutes was added dropwise on the functionalized nanopore channel for current detection on both sides of the membrane.

[0027] Such as Figure 4 As shown, the calculation result of the current change rate of the normal population is 1.28-2.00; the result of oral squamous cell carcinoma patients is: 0.60-1.27; the result of oral squamous cell carcinoma with metastasis is less than 0.60.

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Abstract

The invention relates to a cysteine protease inhibitor B detection device as well as a preparation method and application thereof, and solves the technical problems that the concentration detection range and the detection change interval of the existing cysteine protease inhibitor B saliva detection method are limited, and the concentration reduction of the cysteine protease inhibitor B cannot be sensitively and specifically detected. The asymmetric nano-pore membrane is a polyethylene terephthalate membrane, the polyethylene terephthalate membrane is provided with an asymmetric nano-pore, and the asymmetric nano-pore is in an asymmetric and continuous conical shape. The invention also provides a preparation method and application thereof. The method can be applied to the field of cysteine protease inhibitor B detection.

Description

technical field [0001] The invention relates to a detection device for saliva components and its preparation method and application, in particular to a cysteine ​​protease inhibitor B detection device and its preparation method and application. Background technique [0002] In recent years, the examination of biological fluids for the detection of oral cancer biomarkers has revolutionized traditional detection methods. After research, it was found that the reduction of the content of a common protein in saliva "Cystatin B: CSTB" is an important target for predicting oral squamous cell carcinoma, so its concentration in saliva was detected in time The changes help to accurately predict the onset, metastasis and prognosis status of oral squamous cell patients. Using a method that can detect the concentration change of CSTB in oral saliva is an effective method for early detection of oral cancer occurrence or recurrence, and is beneficial to clinicians' diagnosis and treatment...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3278G01N27/48
Inventor 卫彦邓旭亮闻利平肖作慧黄晨燕孔祥玉
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
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