Biological fermentation composition with anti-cancer effect as well as preparation method and application thereof

A technology of composition and composite fermentation bacteria, which is applied in the field of bioengineering, can solve the problems of pesticides, fertilizers, hormones, pollution, ecological environment damage, etc., and achieve good inhibitory and therapeutic effects, broad application prospects, and simple preparation methods.

Active Publication Date: 2021-06-01
天津市宝恒生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The advancement of science and technology has brought convenience and speed to people's lives, and has greatly improved people's material living standards, but it has also brought a series of negative problems: ecological environment damage and pollution, water pollution, pesticides The harm of fertilizer hormones, the abuse of various additives in food, the abuse of antibiotics, etc.
At present, there are no drugs for the prevention and treatment of cancer on the market, which has brought harm to people's health

Method used

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  • Biological fermentation composition with anti-cancer effect as well as preparation method and application thereof
  • Biological fermentation composition with anti-cancer effect as well as preparation method and application thereof
  • Biological fermentation composition with anti-cancer effect as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of embodiment 1 selenium-enriched natto fermented extract

[0032] Specifically include the following steps:

[0033] S1. Preparation of liquid medium: 5g fructose, 1g ammonia water, 1g threonine, 1g valine, 0.1g vitamin E, 0.2g calcium chloride, 0.1g magnesium sulfate, 0.2g ferric chloride were used in 100mL sterile Dissolve in water, mix evenly, adjust the pH value of the medium to 6.8 with PBS solution, and sterilize with ultraviolet light for later use;

[0034] S2. Cultivation of compound fermentation bacteria liquid: Inoculate 2g of Monascus, 5g of bacterial strain Bacillus natto 918 and 1g of selenium-enriched yeast into Gao's culture medium and streak respectively, and perform aerobic culture for the first time. The culture condition is 35°C, oxygen The content is 20%, the humidity is 70%, the cultivation time is 12h, and then cultivated into the strain seed liquid respectively, and the bacterial content of the strain seed liquid is 10% ...

Embodiment 2

[0038] The preparation method of embodiment 2 selenium-enriched natto fermented extract

[0039] Specifically include the following steps:

[0040] S1. Preparation of liquid medium: mix 15g glucose, 5g urea, 1g ammonium nitrate, 1g glutamic acid, 0.1g vitamin K, 0.1g vitamin B2 and 0.2gg potassium chloride, 0.2g calcium chloride, 0.2g magnesium sulfate , 0.2g ferric chloride, 0.2g zinc sulfate are dissolved with 100mL sterile water, after mixing evenly, adjust the pH value of the culture medium with PBS solution to 7.2, and ultraviolet sterilize for later use;

[0041] S2. Cultivation of compound fermentation bacteria liquid: inoculate 5g of Monascus, 10g of strain Bacillus natto 918 and 3g of selenium-enriched yeast into Gao's culture medium and mark the line respectively, the first aerobic culture, the culture condition is 40 ℃, oxygen The content is 30%, the humidity is 80%, the cultivation time is 24h, and then cultivated into the strain seed liquid respectively, and the ...

Embodiment 3

[0045] The preparation method of embodiment 3 selenium-enriched natto fermented extract

[0046] Specifically include the following steps:

[0047] S1. Preparation of liquid medium: use 10g maltose, 3g urea, 1g leucine, 1g alanine, 0.1g vitamin C, 0.05g vitamin B1, 0.2g zinc sulfate, 0.3g copper sulfate, 0.2g manganese sulfate Dissolve in 100mL sterile water, mix well, adjust the pH value of the medium to 7 with PBS solution, and sterilize with ultraviolet light for later use;

[0048] S2. Cultivation of compound fermentation bacteria liquid: Inoculate 3.5g of Monascus, 7g of strain Bacillus natto 918 and 2g of selenium-enriched yeast into Gao’s culture medium and streak respectively, perform aerobic culture for the first time, and the culture condition is 37°C. Oxygen content is 25%, and humidity is 75%, and culture time is 18h, and then respectively cultures into bacterial seed liquid, and the bacterial content of bacterial seed liquid is 10 8 cfu / mL, inoculated in the liq...

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Abstract

The invention provides a biological fermentation composition with an anti-cancer effect as well as a preparation method and application thereof, and belongs to the technical field of biological engineering. The biological fermentation composition with the anticancer effect is prepared from the following raw materials in parts by weight: 50 to 100 parts of selenium-enriched natto fermentation extract, 2 to 5 parts of ginsenoside Rg2, 1 to 3 parts of ginsenoside Rg3, 2 to 5 parts of notoginsenoside R1 and 4 to 10 parts of cordyceps polysaccharide. The preparation method is simple, the raw materials are wide in source, and the prepared composition has a very good anti-tumor effect, particularly has very good inhibition and treatment effects on thyroid cancer, is safe and free of side effects, can be used as raw materials of dietary therapy products, health-care foods and medicines, and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a biofermentation composition with an anticancer effect and a preparation method and application thereof. Background technique [0002] The advancement of science and technology has brought convenience and speed to people's lives, and has greatly improved people's material living standards, but it has also brought a series of negative problems: ecological environment damage and pollution, water pollution, pesticides The harm of fertilizer hormones, the abuse of various additives in food, the abuse of antibiotics, etc. It has brought damage to the ecological environment of people's life. At the same time, coupled with the fast-paced life of modern people, the radiation damage of Internet surfers, the invasion of influenza viruses, and the increase in work pressure, most people's body immunity has gradually decreased. Sub-health status, what is more worrying is that cancer,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61K31/704A61K31/715A61P35/00A23L33/105A23L33/125
CPCA61K36/48A61K31/704A61K31/715A61P35/00A23L33/105A23L33/125A61K2236/19A23V2002/00A61K2300/00A23V2200/308A23V2250/21A23V2250/51
Inventor 李宝恒李倩舒威
Owner 天津市宝恒生物科技有限公司
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