Technology for promoting tillering of daylily
A technology for day lily, which is applied in the fields of seed immunization, plant regeneration, application, etc., can solve the problems of scientific management of difficult tillering characteristics, and the inability to fully exert and utilize the effects of day lily tillering to increase production and efficiency, and achieve the rate of tillering and bolting Improve and promote root proliferation and growth, the effect of a simple method
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Embodiment 1
[0034] Include the following steps:
[0035] S1. Select the seeds of Mengzihua variety to carry out disinfection treatment, and the disinfection treatment is to soak the seeds in 2wt% hydrogen peroxide for 2 hours;
[0036] S2. Inoculate the seedlings after the disinfection treatment on the bud induction medium, and send them to the culture room for bud induction cultivation;
[0037] The bud induction medium is prepared from the following raw materials by weight: 2 parts of glutamic acid, 1 part of lysine, 2 parts of tryptophan, 10 parts of glucose, 5 parts of calcium phosphate, 1 part of magnesium chloride, 0.5 parts of copper sulfate, 1 part of potassium nitrate, 3 parts of potassium sulfate and 100 parts of water; bud induction culture conditions: temperature is 20 ℃, humidity is 30%;
[0038] S3. transplanting the differentiated seedlings onto the differentiation medium, and continuing to cultivate to obtain differentiated seedlings;
[0039] S4. Dig out the differentia...
Embodiment 2
[0052] Include the following steps:
[0053] S1. Select the seeds of Mengzihua variety to carry out disinfection treatment, and the disinfection treatment is to soak the seeds in 5wt% hydrogen peroxide for 5 hours;
[0054] S2. Inoculate the seedlings after the disinfection treatment on the bud induction medium, and send them to the culture room for bud induction cultivation;
[0055] The bud induction medium is prepared from the following raw materials by weight: 5 parts of glutamic acid, 3 parts of lysine, 5 parts of tryptophan, 20 parts of glucose, 10 parts of calcium phosphate, 2 parts of magnesium chloride, 1 part of copper sulfate, 2 parts of potassium nitrate, 7 parts of potassium sulfate and 150 parts of water; bud induction culture conditions: temperature is 35 ℃, humidity is 50%;
[0056] S3. transplanting the differentiated seedlings onto the differentiation medium, and continuing to cultivate to obtain differentiated seedlings;
[0057] S4. The differentiated see...
Embodiment 3
[0070] Include the following steps:
[0071] S1. Select the seeds of Mengzihua variety to carry out disinfection treatment, and the disinfection treatment is to soak the seeds in 3.5wt% hydrogen peroxide for 3.5h;
[0072] S2. Inoculate the seedlings after the disinfection treatment on the bud induction medium, and send them to the culture room for bud induction cultivation;
[0073] The bud induction medium is prepared from the following raw materials by weight: 35 parts of glutamic acid, 2 parts of lysine, 3.5 parts of tryptophan, 15 parts of glucose, 7 parts of calcium phosphate, 1.5 parts of magnesium chloride, 0.75 parts of copper sulfate, 1.5 parts of potassium nitrate, 5 parts of potassium sulfate and 125 parts of water; bud induction culture conditions: temperature is 30 ℃, humidity is 40%;
[0074] S3. transplanting the differentiated seedlings onto the differentiation medium, and continuing to cultivate to obtain differentiated seedlings;
[0075] S4. The different...
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