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A method for rapidly detecting grape flower buds

A grape flower and grape technology, which is applied to measuring devices, testing plant materials, instruments, etc., can solve the problems of long time, many reagents, carcinogenic and toxic substances, etc., and achieves a simple and easy method, accurate and reliable judgment, high efficiency and accuracy. Effect

Active Publication Date: 2022-05-17
CHANGLI INST OF POMOLOGY HEBEI ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the different number of single buds contained in the bud eye, the external shape and size are different. When using the external shape observation method, although it is simple and quick, there are large errors in the identification of grape flower buds, resulting in inaccurate results.
In addition, the paraffin section method can accurately determine grape flower buds, but this method is cumbersome to operate and needs to go through the processes of fixation, wax soaking, embedding, trimming, dyeing and decolorization, which takes a long time, and the process takes about 15-20 days. The test operators have high technical requirements, are unskilled in operation, and are not easy to obtain slices; in addition, many reagents are used, including carcinogens (formaldehyde) and toxic substances (xylene), which pollute the environment and are not conducive to the health of the experimenters

Method used

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  • A method for rapidly detecting grape flower buds
  • A method for rapidly detecting grape flower buds
  • A method for rapidly detecting grape flower buds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The test materials were provided by the viticulture base of Changli Fruit Tree Research Institute, Hebei Academy of Agriculture and Forestry Sciences. Three grape varieties of Xiahei, Baoguang and Miguang cultivated in the field were selected, and the proportions of flower buds at the 4-5, 5-6 and 10-11 nodes of the branches were counted. According to the following "Flower Bud Rapid Detection Method" (also known as anatomical observation method) and paraffin section method to carry out comparative tests. Both methods were performed in 3 replicates, each containing 10 shoots (20 buds). Specifically, the flower bud detection method is as follows:

[0041](1) At the beginning of October, 180 branches (10 days before winter pruning) were randomly collected, including 60 summer black branches (divided into 2 groups) and 60 Baoguang branches (divided into 2 groups) without damage by diseases and insect pests. ), 60 Miguang branches (divided into 2 groups), one group uses th...

Embodiment 2

[0062] Obtain the original pictures of different buds under the stereomicroscope according to the same method as in Example 1, but when judging the flower buds / leaf buds, use existing software (such as PS software or ImageJ developed by java) or equipment (color difference meter) to compare the stereoscopic images. Numerical conversion (quantification) of the color of the growth point and the surrounding part on the microscope image, and the quantified numerical value is associated with the flower bud judgment result or the leaf bud judgment result, and the computer program is trained. Through the self-learning ability of the computer program, use The computer level directly reads the stereomicroscope pictures, and realizes the automatic judgment of the stereomicroscope pictures of buds.

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Abstract

The invention relates to a method for quickly detecting grape flower buds, comprising the following steps: S1 collecting vigorously growing and pest-free branches of grapes after lignification and before pruning in winter; S2 moisturizing grape flower bud branches; S3 using scissors to measure grape branches Cut into 4-5cm long stems according to the nodes, wrap the cut stems with buds with moist gauze to be tested; S4 remove the buds from the stems, and cut in the direction of the knife when taking the buds Perpendicular to the bud body and make the bottom of the bud body smooth and flat; cross-cut the removed bud body with a knife, and the cross-section is parallel to the plane of the bottom of the bud body; after cross-cutting, put it under a stereo microscope to observe the cross-section surface, and the observation magnification is 2‑4 Take the tender green central point on the cross-section as the growth point, if the distribution of scales around the growth point is uneven and obvious irregular radial protrusions are observed, it is the inflorescence primordia, and it is judged as a flower bud; otherwise, it is judged as a leaf bud. The invention has the characteristics of "accurate and reliable judgment, fast and efficient, safe and non-toxic, low cost and simple operation, and suitable for rapid detection in large quantities".

Description

technical field [0001] The invention relates to a method for detecting flower buds of fruit trees, in particular to a method for rapidly detecting flower buds of grapes. Background technique [0002] Grape is one of the important fruit trees in my country, and the quality and quantity of its flower buds are directly related to the output of grapes in the second year. The flower bud differentiation of grapes is divided into two stages as a whole. The first stage is the inflorescence differentiation stage, which is completed in the year when the new shoot grows. This is the key stage to determine whether the grape has flower buds. In spring, it is completed before and after budding to before flowering, and the differentiation stage is concentrated and short, which is the key stage to determine the quality of flower buds. In grape production, how to quickly and accurately judge flower buds plays an important role in guiding annual grape production and predicting grape yield. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84
CPCG01N21/84G01N2021/8466
Inventor 贾楠尹勇刚郭紫娟韩斌刘长江孙艳李敏敏赵胜建
Owner CHANGLI INST OF POMOLOGY HEBEI ACADEMY OF AGRI & FORESTRY SCI