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Method for optimizing compound enzyme-assisted ultrasonic extraction of China rose total flavonoids by response surface method

A technology of response surface method and compound enzyme, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., can solve problems such as low test accuracy, poor predictability of mathematical models, and long extraction time

Inactive Publication Date: 2021-06-08
TONGREN POLYTECHNIC COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The two commonly used methods of uniform design and orthogonal design have shortcomings such as low test accuracy and poor predictability of the established mathematical model.
[0004] The patent document with the publication number CN1772065A discloses a preparation method of total flavonoids of rose flower, which adopts the cold soaking method to extract the total flavonoids of rose flower, but the cold soaking method has the disadvantages of long extraction time and large amount of solvent.

Method used

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  • Method for optimizing compound enzyme-assisted ultrasonic extraction of China rose total flavonoids by response surface method
  • Method for optimizing compound enzyme-assisted ultrasonic extraction of China rose total flavonoids by response surface method
  • Method for optimizing compound enzyme-assisted ultrasonic extraction of China rose total flavonoids by response surface method

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Experimental program
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Effect test

Embodiment 1

[0051] Chinese rose is dried, oven-dried, and it is pulverized into 80 mesh particles. Weigh 10.0000g of Chinese rose powder, add 100ml of citric acid-sodium citrate buffer solution with a pH value of 4.5, add 150mg of compound enzyme (cellulase: pectinase = 3:2), and enzymatically hydrolyze at 45°C for 60min. Collect the extract, filter to obtain the crude extract, use a rotary evaporator to concentrate the extract to 30ml, add petroleum ether to wash three times, discard the petroleum ether part, add ethyl acetate to the water phase for extraction three times, and combine the ethyl acetate part , evaporated to dryness in a water bath, add ethanol solution and transfer to a constant volume in a 100ml volumetric flask, and dilute to volume with ethanol to obtain the test solution. Put 0.5ml of rose flower test solution into a 10ml volumetric flask, then add 0.4ml of 5% sodium nitrite in sequence, leave it for 6 minutes, add 0.4ml of 10% aluminum nitrate, leave it for 6 minutes...

Embodiment 2

[0053] Chinese rose is dried, oven-dried, and it is pulverized into 80 mesh particles. Weigh 10.0000g of Chinese rose powder, add 100ml of citric acid-sodium citrate buffer solution with a pH value of 4.5, add 200mg of compound enzyme (cellulase: pectinase = 3:2), and enzymatically hydrolyze at 45°C for 50min. Collect the extract, filter to obtain the crude extract, use a rotary evaporator to concentrate the extract to 30ml, add petroleum ether to wash three times, discard the petroleum ether part, add ethyl acetate to the water phase for extraction three times, and combine the ethyl acetate part , evaporated to dryness in a water bath, add ethanol solution and transfer to a constant volume in a 100ml volumetric flask, and dilute to volume with ethanol to obtain the test solution. Put 0.5ml of rose flower test solution into a 10ml volumetric flask, then add 0.4ml of 5% sodium nitrite in sequence, leave it for 6 minutes, add 0.4ml of 10% aluminum nitrate, leave it for 6 minutes...

Embodiment 3

[0055] Chinese rose is dried, oven-dried, and it is pulverized into 80 mesh particles. Weigh 10.0000g of Chinese rose powder, add 100ml of citric acid-sodium citrate buffer solution with a pH value of 5.0, add 200mg of compound enzyme (cellulase: pectinase = 3:2), and enzymolyze at 45°C for 40min. Collect the extract, filter to obtain the crude extract, use a rotary evaporator to concentrate the extract to 30ml, add petroleum ether to wash three times, discard the petroleum ether part, add ethyl acetate to the water phase for extraction three times, and combine the ethyl acetate part , evaporated to dryness in a water bath, add ethanol solution and transfer to a constant volume in a 100ml volumetric flask, and dilute to volume with ethanol to obtain the test solution. Put 0.5ml of the Chinese rose test solution into a 10ml volumetric flask, then add 0.4ml of 5% sodium nitrite in turn, leave it for 6 minutes, add 0.4ml of 10% aluminum nitrate, leave it for 6 minutes, and finall...

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Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to a method for optimizing compound enzyme-assisted ultrasonic extraction of China rose total flavonoids by a response surface method. Process parameters of enzymolysis-assisted ultrasonic extraction of China rose total flavonoids are defined by the response surface method, all factors and interaction pairs of every two factors can be fully considered to be fully compensated and perfected, one-sidedness and discretization existing in orthogonal design adjustment parameters after single-factor test treatment are avoided, the optimal process parameters for extracting the China rose total flavonoids are obtained by the response surface method, and the process is efficient, controllable, stable and feasible.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for optimizing composite enzyme-assisted ultrasonic extraction of total flavonoids from rose flowers by using a response surface method. Background technique [0002] Rosaceae belongs to Rosaceae. It is sweet in taste and warm in nature. The roots, leaves and flowers can be used as medicine. It has the effects of promoting blood circulation and regulating menstruation, soothing the liver and relieving depression. Chinese rose contains active ingredients such as flavonoids, volatile oils, and pigments. Pharmacological studies have shown that flavonoids in roses have various activities such as anti-oxidation, anti-fungal, and hypoglycemic. [0003] The response surface method uses a reasonable experimental design method and obtains certain data through experiments, and uses multiple quadratic regression equations to fit the functional relationship betw...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/738A61K133/00
CPCA61K36/738A61K2236/19A61K2236/333A61K2236/51
Inventor 宁娜韩建军李广平付才进
Owner TONGREN POLYTECHNIC COLLEGE
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