Probiotic for regulating and controlling microecological balance of epidermis as well as product and application thereof
A technology of ecological balance and probiotics, applied in the field of microorganisms, can solve the problems of product quality control, storage and transportation of live bacteria products, and achieve the effect of significantly regulating the microecology of the epidermis and treating and caring for acne
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Embodiment 1
[0044] The separation and purification of embodiment 1 probiotics and species preservation
[0045] The laboratory collected milk samples from healthy lactating women. After gradient dilution with sterile anaerobic water, the appropriate gradient dilution was selected and spread on the MRS solid medium, and anaerobically incubated at 37°C for 48 hours. Pick typical colonies and streak them on MRS solid medium for 2-3 times, and cultivate to obtain pure colonies. It was identified as a strain of Bifidobacterium animalis by 16S rDNA molecular identification, named Bifidobacterium animalis 5b5l3 (Bifidobacterium animalis 5b5l3), and the strain was deposited in China Center for Type Culture Collection CCTCC on April 16, 2021.
Embodiment 2
[0046] The fermented liquid preparation of embodiment 2 probiotics
[0047] (1) Activation of bacteria
[0048] Take out the Bifidobacterium animalis 5b5l3 glycerol cryopreservation tube stored in the -80°C refrigerator, put it at room temperature to thaw, pick up the strains with an inoculation loop in a sterile environment, inoculate them on the MRS solid plate, and incubate at 37°C for 48 hours After observing the colony morphology on the plate and confirming that it is Bifidobacterium animalis without pollution, pick a single bacterium on the plate and inoculate it into MRS liquid medium, and cultivate it at 37°C for 24 hours for later use.
[0049] (2) Preparation of fermentation medium
[0050] Weigh 10g sucrose, 5g soybean meal, 5g corn, 5g bran, 5g NaCl, 0.5g MgSO4, add deionized water to 500mL, and adjust the pH to 5-6.
[0051] (3) Preparation of fermented nutrient material
[0052] Weigh and wash 5-10g of rice, soak in 500mL of deionized water for 2 hours, then add...
Embodiment 3
[0060] The bacteriostatic effect test of embodiment 3 probiotic fermented liquid
[0061] The probiotic fermentation base liquid prepared in Example 2 and the probiotic fermentation liquid were respectively added to BHI and LB liquid medium according to the volume ratio of 5%, and the inoculum of Propionibacterium acnes was inoculated into In the BHI medium of the probiotic fermentation base liquid and the probiotic fermentation liquid, Staphylococcus epidermidis was inoculated into the LB medium containing the probiotic fermentation base liquid and the probiotic fermentation liquid prepared in Example 2 with an inoculum size of 2%. ; Negative control groups were BHI and LB liquid medium, respectively inoculated Propionibacterium acnes and Staphylococcus epidermidis with 2% inoculum, and the inoculation conditions of the medium were shown in the table below. Incubate at 37°C for 24 hours, OD 600 Detect the absorbance value. Three parallel experiments were set up for each gro...
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