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Method for constructing intestinal mucositis animal model

A technology of animal models and construction methods, applied in the field of biomedicine, which can solve problems such as lack of typicality

Active Publication Date: 2021-06-18
澎立生物医药技术(上海)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The film-forming rules of animal models of radiation colitis, but most of them are not typical for the study of the disease mechanism of ordinary mild chronic enteritis, and are mainly used to explore the impact of enteritis and repeated intestinal tissue lesions

Method used

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  • Method for constructing intestinal mucositis animal model
  • Method for constructing intestinal mucositis animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of construction method of intestinal mucositis animal model

[0036] 1. Experimental reagents: Harris hematoxylin solution preparation (including A liquid hematoxylin 1g and absolute alcohol 10ml, B liquid potassium aluminum sulfate 20g and ultrapure water 200ml)

[0037] Preparation method: weigh KAl(SO 4 ) 2 12H 2 O 36.72g and hematoxylin 1g were dissolved and mixed respectively, heated and boiled until the solid was dissolved, slowly added 0.5g of mercuric oxide, and the mixed liquid was quickly cooled down, and the cooled liquid was filtered with a 0.45mm filter membrane. When ready to use, add 6ml of glacial acetic acid and 10ml of glycerin (glycerol) per 100ml to mix and prepare for use.

[0038] Bouin's fixative solution: mix 75ml of picric acid, 25ml of 40% formaldehyde solution and 5ml of glacial acetic acid.

[0039] 2. Selection and grouping of experimental animals: 20 SPF level BALB / c mice aged 9-10 weeks were randomly divided into control group a...

Embodiment 2

[0070] Large-scale administration of the flora in the intestinal tract at the same time will cause physical discomfort or death of the mice, while administration of small doses may cause the administered flora to be killed by the body's own immune system, resulting in failure of flora administration. Based on the long-acting and continuous administration of a small amount of flora, the present invention adopts a bacterial liquid capsule. The bacterial liquid capsule is sealed and wrapped with a polymer osmotic membrane, which can slowly release the bacterial liquid wrapped inside through the osmosis of the osmotic membrane or self-decomposition. Since the size of bacteria is generally between 0.5 and 7 μm, the pore size of the controlled-release membrane or slow-release membrane should be set according to the bacterial liquid to be wrapped. For example, the volume of E. The pore size of the permeable membrane can be 3 μm. When the wrapped bacterial solution is a mixed bacteri...

Embodiment 3

[0075] This embodiment discloses a capsule administration device. The applicator comprises a fixing unit 100 , a connecting rod 200 and a handle 300 . The fixing unit 100 can clamp the capsule, so that the fixing unit 100 releases the capsule when the capsule is at a predetermined position in the intestinal tract, such as figure 1 As shown; the connecting rod 200 can change with the bending shape of the intestinal tract when it enters the intestinal tract, and the connecting rod 200 is provided with electronic detection or scale, so that the user can know the length of the administration device entering the mouse body, and release it at a fixed point Capsules, since the user cannot clearly perceive where the capsule enters the intestinal tract when administering the capsule to mice, it is necessary to design a tool that can assist the user to understand the depth of the intestinal tract where the capsule is located. The handle 300 is held by the user to help control the appli...

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Abstract

The invention relates to a method for constructing an intestinal mucositis animal model. The method comprises the following steps of carrying out flora inoculation on the intestinal tract of an animal sample to induce the intestinal tract of the animal sample to generate inflammation, carrying out character observation, physiological and biochemical index detection and rectal middle section mucosa tissue structure microscopic observation on an inoculated mouse, when bloody stool or mucus stool appears in the mouse, and physiological and biochemical indexes related to inflammatory factors rise, or the middle section of rectum mucosa tissue skin lesion or inflammation infiltration symptoms appear, successfully constructing the intestinal mucositis animal model. Through a controlled-release or slow-release intestinal suppository application mode, uncontrollable factors generated in the application process are solved, the operation of bacterial liquid inoculation is unified and simplified, and the construction of the intestinal mucositis animal model can be repeated.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for constructing an animal model of intestinal mucositis. Background technique [0002] Enteritis is enteritis and colitis caused by bacteria, viruses, fungi and parasites. Clinical manifestations mainly include abdominal pain, diarrhea, watery stools or mucus, pus and blood in stools. Some patients may have fever and tenesmus, so it is also called infectious diarrhea. Enteritis is divided into acute and chronic types according to the length of the course of the disease. The causes of enteritis mainly include viral enteritis, bacterial enteritis, fungal enteritis, parasitic enteritis, enteritis caused by diet and enteritis caused by antibiotics, among which Shigella, Salmonella, Campylobacter jejuni and Escherichia coli are caused Bacterial enteritis is the most common. [0003] Currently, the animal models used in the research of enteritis diseases are mainly divided into...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 段继峰戴飞红俞诚杨平
Owner 澎立生物医药技术(上海)股份有限公司
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