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A method for constructing an animal model of intestinal mucositis

A technology of animal models and construction methods, applied in the field of biomedicine, which can solve problems such as lack of typicality

Active Publication Date: 2022-08-05
澎立生物医药技术(上海)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The film-forming rules of animal models of radiation colitis, but most of them are not typical for the study of the disease mechanism of ordinary mild chronic enteritis, and are mainly used to explore the impact of enteritis and repeated intestinal tissue lesions

Method used

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  • A method for constructing an animal model of intestinal mucositis
  • A method for constructing an animal model of intestinal mucositis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for constructing an animal model of intestinal mucositis

[0036] 1. Experimental reagents: Harris hematoxylin solution (contains 1g of hematoxylin in solution A and 10ml of anhydrous alcohol, and 20g of potassium aluminum sulfate in solution B and 200ml of ultrapure water)

[0037] Preparation method: weigh KAl (SO 4 ) 2 ·12H 2 O 36.72g and hematoxylin 1g were dissolved and mixed respectively, heated and boiled until the solid dissolved, slowly added 0.5g of mercuric oxide, the mixed liquid was rapidly cooled and cooled, and the liquid was filtered using a 0.45mm filter membrane after cooling. When ready to use, mix and configure according to the ratio of adding 6 ml of glacial acetic acid and 10 ml of glycerol (glycerol) per 100 ml.

[0038] Bouin's fixative: Mix 75ml of picric acid, 25ml of 40% formaldehyde solution and 5ml of glacial acetic acid.

[0039] 2. Selection and grouping of experimental animals: 20 SPF grade BALB / c mice aged 9-10 weeks were ra...

Embodiment 2

[0070] Simultaneous administration of a large area of ​​the flora in the intestinal tract can lead to physical discomfort or death of the mouse, while the administration of a small dose may cause the administered flora to be killed by the body's own immune system, resulting in the failure of the flora administration. Based on the long-acting and uninterrupted microflora application mode, the present invention adopts a bacterial liquid capsule. The bacterial liquid capsule is sealed and encapsulated by a polymer permeable membrane, which can slowly release the encapsulated bacterial liquid through the osmosis or self-decomposition of the permeable membrane. Since the size of bacteria is generally between 0.5 and 7 μm, the pore size of the controlled-release membrane or sustained-release membrane should be set according to the bacterial liquid to be wrapped. The permeable membrane pore size can be 3 μm. When the encapsulated bacterial solution is a mixed bacterial solution, the...

Embodiment 3

[0075] This embodiment discloses a capsule administration device. The applicator includes a fixing unit 100 , a connecting rod 200 and a handle 300 . The fixing unit 100 can hold the capsule, so that the fixing unit 100 can release the capsule when the capsule is in a predetermined position in the intestine, such as figure 1 The connecting rod 200 can change with the bending shape of the intestinal tract when entering the intestinal tract, and the connecting rod 200 is provided with electronic detection or scale, so that the user can know the length of the applicator entering the mouse and release it at a fixed position Capsules, since the user cannot clearly perceive the location of the capsule entering the intestine when administering the capsule to the mouse, it is necessary to design a tool that can assist the user to understand the depth of the intestine where the capsule is located. The handle 300 helps control the applicator by the user's hand. The handle 300 is provi...

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Abstract

The invention relates to a method for constructing an animal model of intestinal mucositis. The intestinal flora of the animal sample is inoculated to induce inflammation in the intestinal tract of the animal sample, and the inoculated mice are subjected to character observation, physiological and biochemical index detection, and the middle rectum. Microscopic observation of mucosal tissue structure shows that the animal model of intestinal mucositis has been successfully constructed when the mice have bloody stools or mucus stools, increased physiological and biochemical indicators related to inflammatory factors, or symptoms of skin lesions or inflammatory infiltration in the middle rectal mucosal tissue. Through controlled-release or sustained-release enteral suppository administration, the uncontrollable factors generated in the administration process are resolved, the operation of bacterial liquid inoculation is unified and simplified, and the model construction of intestinal mucositis animals can be reproducible.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for constructing an animal model of intestinal mucositis. Background technique [0002] Enteritis is enteritis and colitis caused by bacteria, viruses, fungi and parasites. The main clinical manifestations are abdominal pain, diarrhea, watery stools or mucus pus and bloody stools. Some patients may have fever and tenesmus, so it is also called infectious diarrhea. Enteritis is divided into acute and chronic categories according to the duration of the disease. The causes of enteritis mainly include viral enteritis, bacterial enteritis, fungal enteritis, parasitic enteritis, enteritis caused by diet and enteritis caused by antibiotics, among which, Shigella, Salmonella, Campylobacter jejuni and Escherichia coli are caused Bacterial enteritis is the most common. [0003] At present, animal models for enteritis disease research are mainly divided into three categories, includi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 段继峰戴飞红俞诚杨平
Owner 澎立生物医药技术(上海)股份有限公司
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