Artemisia sieversiana crude extract and metarhizium anisopliae compound bait for preventing and treating oedaleus asiaticus and application thereof
A technology of locust and Artemisia grandis, which is applied in the field of compound bait of crude extract of Artemisia grandis and Metarhizium anisopliae, can solve the problem of less research on the synergistic effect of insecticidal active fungi and insecticides, and achieve good control effect, improve the control effect, increase the effect of force
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[0020] 3. Preparation of Crude Extract of Artemisia grandis
[0021] Use water extraction method to extract plant crude extract: Cut the fresh plant material Artemisia grandis into small pieces of about 1cm and put it into a conical flask, add according to the mass ratio of distilled water to Artemisia grandis as 1:9 or 2:8 After distilling water, put it in an autoclave and heat it at 120°C for 30 minutes; set two concentrations of 10% and 20% water extracts of Artemisia grandis; take it out after heating, cool to room temperature, and filter the plant residue.
Embodiment 1
[0022] Embodiment 1 Artemisia grandis and Metarhizium anisopliae single agent and mixed agent lethal effect
[0023] The 4th instar nymphs of P. asiatica collected in the field were pre-starved for 24 hours, and 20 healthy P. asiatica (female to male ratio 1:1) were picked and put into sterile plastic baskets, with 5 replicates for each treatment. 10% and 20% crude extracts of Artemisia grandis were dipped with a brush, and evenly brushed on Stipa cruzi for feeding, and water was used as a blank control; the gradient concentration of Metarhizium anisopliae was 10 6 ,10 7 ,10 8 (spores / g) were mixed with 5 mg of wheat bran (Table 1), and fed with fresh Stipa kirsenii after 24 hours, and the data were observed and recorded. The experiment lasted for one week. The experiment was carried out in a 16L:8D chamber at 30°C and repeated three times. The mortality rate under each treatment was recorded every day, as shown in Table 2.
[0024] Table 1 The components and concentration...
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