Method for detecting small molecules in milk based on centrifugal microfluidics, and special chip thereof

A microfluidic chip and chip technology, applied in the direction of measuring devices, analysis materials, instruments, etc., can solve the problems of cumbersome steps, easy to cause errors, complicated operations, etc., and achieve the effect of simplifying experimental steps, reducing errors, and simplifying operation steps

Active Publication Date: 2021-06-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a centrifugal microfluidic-based detection of small molecules in milk that can realize mechanical operation

Method used

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  • Method for detecting small molecules in milk based on centrifugal microfluidics, and special chip thereof
  • Method for detecting small molecules in milk based on centrifugal microfluidics, and special chip thereof
  • Method for detecting small molecules in milk based on centrifugal microfluidics, and special chip thereof

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Experimental program
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Effect test

Embodiment 1

[0038] like figure 1 As shown, the present invention provides a microfluidic chip, the chip is provided with: a sample pool 1, an immunomagnetic bead liquid is housed in the sample pool 1, and a sample hole is provided on the upper surface of the sample pool 1 10; eluent pool 3, eluent is housed in the said eluent pool 3, said eluent pool 3 communicates with sample pool 1 through micro flow channel 9, and said eluent pool 3 is located in the sample Above the pool 1, the outlet 31 of the eluent pool 3 flowing to the sample pool 1 is located at the lower end of the eluent pool 3; the waste liquid pool 2, the waste liquid pool 2 and the sample pool 1 pass through the micro flow channel 9 The waste liquid pool 2 and the eluent pool 3 are located at the upper and lower sides of the sample pool 1; and the waste liquid pool 2 is located at the lower left side of the sample pool 1, and the sample pool 1 flows to the The liquid outlet 11 is located at the lower left corner of the samp...

Embodiment 2

[0049] The present invention also provides a centrifugal microfluidic system, including the microfluidic chip and a biaxial centrifugal platform, and the biaxial centrifugal platform includes a chip base (such as figure 2 As shown), the chip base is fixedly provided with a positioning column, and the magnet can reciprocate in the magnet positioning hole of the microfluidic chip.

[0050] The biaxial centrifugal platform refers to a platform including a rotating shaft and a centrifugal shaft at the same time. The centrifugal shaft drives the rotating shaft, the chip base and the microfluidic chip to be centrifuged, and the rotating shaft drives the chip base and the microfluidic chip to rotate. The chip The base is fixed with a positioning column, and the magnet passes through the chip base to reciprocate in the magnet positioning hole of the microfluidic chip. When the rotating shaft drives the chip base and the microfluidic chip to rotate, the magnet moves in the magnet posit...

Embodiment 3

[0053] Using the above-mentioned centrifugal microfluidic system to conduct a centrifugal microfluidic-based detection method for small molecules in milk, including:

[0054] 1) Put 500uL of the milk sample to be tested in the sample pool of the microfluidic chip;

[0055] 2) Place the microfluidic chip on the centrifuge platform, and rotate the chip forward and reverse to mix the sample with the immunomagnetic bead liquid to obtain the first mixed solution;

[0056] 3) The rotation axis of the dual-axis centrifugal platform rotates 60° (-60°) counterclockwise, and the microfluidic chip rotates until the magnet is located at the top of the side of the magnet positioning hole close to the waste liquid pool. At this time, the waste liquid pool is located on the chip away from the centrifuge Fix the chip at the center of the shaft 101 for centrifugation. Under the action of centrifugal force, the separated liquid flows into the waste liquid pool. At this time, the positional rela...

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Abstract

The invention provides a method for detecting small molecules in milk based on centrifugal microfluidics, and a special chip thereof. The whole detection method including pretreatment and test strip detection is completed through a centrifugal microfluidic system, manual operation is replaced by mechanical operation, the detection efficiency is greatly improved, the operation steps are simplified, and sources causing errors are reduced. Experiment steps can be effectively simplified, manual operation is replaced by mechanical operation, errors caused by manual operation are reduced, and the detection precision is improved. In the centrifugal microfluidic system, the microfluidic chip is used in cooperation with double-shaft centrifugal platform, the special structure of the microfluidic chip is matched with a rotating shaft and a centrifugal shaft of a double-shaft centrifugal platform, fluid is directionally driven, meanwhile, effective separation of magnetic beads and liquid can be achieved through the magnet, and in addition, the infrared light source heating function of the double-shaft centrifugal platform enables a liquid to be heated to release small molecules.

Description

technical field [0001] The invention belongs to the field of biological detection methods, and in particular relates to a method for detecting small molecules in milk based on centrifugal microfluidics and a special chip thereof. Background technique [0002] The traditional method for detecting small molecule toxic and harmful substances in milk is as follows: firstly, the milk to be tested is pretreated by the milk magnetic bead method: including mixing of the sample and magnetic beads, separation, eluent elution, and re-separation of magnetic beads , and finally obtain the sample liquid to be tested; and then use a rapid detection test strip (such as a quinolone rapid detection test strip) to perform a rapid qualitative detection of the pretreated sample liquid to be tested. [0003] The above-mentioned pre-processing steps are complex and require multiple manual operations, which not only causes a waste of time and experience in human resources, but also affects the effe...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/54326
Inventor 余文博沈建忠温凯王战辉江海洋于雪芝张素霞
Owner CHINA AGRI UNIV
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