RGD/PTX-coated ZIF-90 drug delivery system and preparation method thereof

A technology of ZIF-90 and delivery system, applied in drug combination, pharmaceutical formulation, antineoplastic drugs, etc., can solve the problems of low bioavailability and toxic side effects, achieve low toxicity and good effect, overcome allergic reactions, and increase release rate Effect

Pending Publication Date: 2021-06-22
ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems of low bioavailability and toxic side effects of paclitaxel injection, which is currently used as a common clinical preparation, and the need for a linker for the targeting of RCD peptides, the present invention provides a novel RGD-paclitaxel-metal organic framework ZIF-90 (RGD / PTX@ZIF-90) drug delivery system and its preparation method, the delivery system uses ZIF-90 as a carrier, and uses RGD to modify by Schiff base reaction, and then forms RGD@ZIF-90 and then adsorbs the antibody by reaction The tumor drug PTX makes it form RGD / PTX@ZIF-90, reduces its toxic side effects and enhances its bioavailability and targeting, improves the therapeutic effect of the drug, and the preparation method is simple

Method used

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  • RGD/PTX-coated ZIF-90 drug delivery system and preparation method thereof
  • RGD/PTX-coated ZIF-90 drug delivery system and preparation method thereof
  • RGD/PTX-coated ZIF-90 drug delivery system and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0054] In this example, the RGD / PTX@ZIF-90 drug delivery system was prepared by the preparation method of the RGD / PTX@ZIF-90 drug delivery system of the present invention.

[0055] The preparation of the RGD / PTX@ZIF-90 drug delivery system specifically includes the following steps:

[0056] (1) Preparation of ZIF-90 nanoparticles

[0057] Dissolve 0.24g of imidazole-2-carboxaldehyde in 12.5mL of pure water, dissolve, add 0.05g of polyvinylpyrrolidone, and ultrasonically dissolve, the ultrasonic time is 30min, which is recorded as solution A;

[0058] Dissolve 0.0282g of zinc nitrate hexahydrate in 12.5mL of tert-butanol, and ultrasonically dissolve it for 30 minutes, and record it as solution B;

[0059] The solutions A and B were mixed and stirred, and the stirring reaction conditions were as follows: the stirring speed was 1000 rpm / min, and the reaction time was 15 min. After the reaction, the solution was centrifuged, the precipitate was washed, and dried to obtain ZIF-90...

Embodiment 2

[0067] In this example, the RGD / PTX@ZIF-90 drug delivery system was prepared by the preparation method of the RGD / PTX@ZIF-90 drug delivery system of the present invention.

[0068] The preparation of the RGD / PTX@ZIF-90 drug delivery system specifically includes the following steps:

[0069] (1) Preparation of ZIF-90 nanoparticles

[0070] Dissolve 0.24g of imidazole-2-carboxaldehyde in 10mL of pure water, dissolve, add 0.05g of polyvinylpyrrolidone, and ultrasonically dissolve for 30min, record as solution A;

[0071] Dissolve 0.02g of zinc nitrate hexahydrate in 10mL of tert-butanol, ultrasonically dissolve for 30min, and record it as solution B;

[0072] The solutions A and B were mixed and stirred, and the stirring reaction conditions were as follows: the stirring speed was 700 rpm / min, and the reaction time was 15 min. After the reaction, the solution was centrifuged, the precipitate was washed, and dried to obtain ZIF-90 nanoparticles.

[0073] (2) Preparation of RGD / Z...

Embodiment 3

[0079] In this example, the RGD / PTX@ZIF-90 drug delivery system was prepared by the preparation method of the RGD / PTX@ZIF-90 drug delivery system of the present invention.

[0080] The preparation of the RGD / PTX@ZIF-90 drug delivery system specifically includes the following steps:

[0081] (1) Preparation of ZIF-90 nanoparticles

[0082] Dissolve 0.24g of imidazole-2-carboxaldehyde in 20mL of pure water, dissolve, add 0.1g of polyvinylpyrrolidone, ultrasonically dissolve, ultrasonic time is 50min, record as solution A;

[0083] Dissolve 0.05g of zinc nitrate hexahydrate in 20mL of tert-butanol, ultrasonically dissolve for 50min, and record it as solution B;

[0084] The solutions A and B were mixed and stirred, and the stirring reaction conditions were as follows: the stirring speed was 1000 rpm / min, and the reaction time was 30 min. After the reaction, the solution was centrifuged, the precipitate was washed, and dried to obtain ZIF-90 nanoparticles.

[0085] (2) Preparat...

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Abstract

The invention discloses an RGD/PTX-coated ZIF-90 drug delivery system and a preparation method thereof, and belongs to the field of biological medicine. According to the drug delivery system, on the basis of the metal organic framework ZIF-90, aldehyde groups and amino groups of RGD are connected through a Schiff base reaction, PTX is entrapped through the adsorption effect, no extra chemical reagent is introduced in the whole process, and the drug delivery system is safer and more environmentally friendly. The preparation method is simple, the ZIF-90 and the RGD are stirred to obtain RGD/ZIF-90, then the RGD/ZIF-90 and the PTX are stirred together, and the RGD/PTX ZIF-90 composite material is obtained. According to the drug delivery system, aiming at the physiological characteristics of specific high expression of an alpha v beta 3 integrin receptor and low pH of tumor cells, RGD can target the tumor cells, and when the RGD reaches the vicinity of the tumor cells, the skeleton of ZIF-90 collapses, so that PTX is intensively released near the tumor cells, the anti-tumor activity is improved, the side effect of the drug can be reduced, and the drug delivery system has the advantages of strong anti-tumor effect, few side effects and high stability.

Description

technical field [0001] The invention belongs to the field of anticancer drug delivery system, and in particular relates to a novel anticancer drug delivery system of RGD-paclitaxel-metal organic framework ZIF-90 (RGD / PTX@ZIF-90) and a preparation method thereof. Background technique [0002] With the development of society and the change of people's lifestyle, cancer has become a major disease that endangers human health. How to effectively control and treat cancer has become an important public health problem to be solved urgently. Although traditional anticancer drugs can prolong the lifespan of some patients, they also face many challenges, such as short half-life, low bioavailability, high drug resistance, nonspecific distribution, and difficulty in crossing physiological barriers, etc. [0003] Paclitaxel (PTX) is a common antineoplastic drug, which is widely used in the treatment of various forms of advanced and refractory cancers. It is a broad-spectrum antineoplasti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/22A61K47/18A61K31/337A61P35/00A61K49/00
CPCA61K47/22A61K47/18A61K31/337A61P35/00A61K49/0043
Inventor 葛飞乔瑞安浩辛影陶玉贵杨凯张伟伟宋平李婉珍朱龙宝
Owner ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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