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Application of complex microbial inoculant containing bile acid in preparation of preparation for preventing and treating fatty liver of pets

A compound bacterial agent and bile acid technology, applied in the field of pet breeding, can solve the problems of liver damage, cumbersome treatment process, and not very obvious effect, so as to promote the excretion of bilirubin, inhibit the deposition, and facilitate the feeding of medicines Effect

Inactive Publication Date: 2021-06-25
SHANDONG LONGCHANG ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In clinical practice, it is necessary to design a treatment plan according to the pet's own condition and the severity of fatty liver. The treatment process is cumbersome and tests the doctor's clinical experience, and the effect is not very obvious. certain damage

Method used

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  • Application of complex microbial inoculant containing bile acid in preparation of preparation for preventing and treating fatty liver of pets
  • Application of complex microbial inoculant containing bile acid in preparation of preparation for preventing and treating fatty liver of pets
  • Application of complex microbial inoculant containing bile acid in preparation of preparation for preventing and treating fatty liver of pets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Screening of Bacteroides polymorpha GL-02

[0030] Take 10g of healthy pig feces and 100mL of sterile water and mix them evenly, filter and remove impurities to make a feces dilution, and then continue to add sterile water to the dilution to prepare 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 diluent; then take 10 -2 、10 -3 、10 -4 、10 -5 Spread the dilution on GAM agar medium containing 80% N 2 , 10% CO 2 and 10%H 2 Under the anaerobic environment, cultivated at 35°C for 48 hours, selected obvious colonies and purified them several times to obtain a single colony, which was named GL-02 and stored in a frozen state. Among them, the formula of GAM agar medium is: 10.0g peptone, 3.0g soytone, 10.0g monthly peptone, 13.5g serum digestion powder, 2.2g beef extract powder, 5.0g yeast extract powder, 1.2g beef liver extract powder, 3.0 g glucose, 3.0g sodium chloride, 2.5g potassium dihydrogen phosphate, 5.0g soluble starch, 0.3g L-cysteine, 0.15g sodium thioglycolate, 15....

Embodiment 2

[0037] Embodiment 2: the preparation of composite bacterial agent

[0038] 1. Preparation of bile acids

[0039] The preparation process steps of described bile acid are as follows:

[0040] (1) Saponification: Add 1 kg of pig gall powder crushed through a 40-mesh sieve to the reaction kettle, then add 10L of sodium hydroxide solution with a mass concentration of 10%, heat and stir until boiling, and keep stirring for 14 hours in a boiling state, and then react The generated saponified liquid is cooled to solid-liquid separation, the supernatant is removed, and the remaining solid is bile acid saponified product;

[0041](2) Decolorization: Add 6L of water to the bile acid saponification product, heat to 80°C to completely dissolve the bile acid saponification product, then pour it into a decolorization tank, add 300mL hydrogen peroxide and stir evenly, react at room temperature for 24 hours, and then put the reaction The solution is filtered into the acidification tank to o...

Embodiment 3

[0053] A pet hospital in Changchun City, Jilin Province received a 2-year-old male blue cat named Groot, weighing 8kg. When he was admitted to the hospital, Groot did not eat and was on a hunger strike. His ears and teeth were yellow and stained. After biochemical testing, the test indicators (Table 1) showed that Groot’s total bilirubin (T-Bil), serum alkali Both sex phosphatase (ALP) and γ-glutamyl transpeptidase (GGT) were significantly elevated, and the liver was significantly enlarged on chest examination ( figure 1 ), the initial diagnosis was hepatic fat deposition.

[0054] For Groot's fatty liver condition, the compound microbial agent prepared in Example 2 was mixed in Groot's cat food at a daily dosage of 5 g, once a day, for 25 consecutive days. In the process of using the compound bacterial agent, after 5 days of use, take Groot to the first visit for biochemical index detection; after 6 days of use, the second visit for biochemical index detection; after 14 days...

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Abstract

The invention discloses application of a complex microbial inoculant containing bile acid in preparation of a preparation for preventing and treating fatty liver of pets. The complex microbial inoculant comprises bacteroides multiforme powder, bile acid and eucalyptus multibracteata essential oil in a mass ratio of (2-3): (2-3): 1; the bacteroides polymorpha powder is prepared by culturing, fermenting and drying bacteroides polymorpha GL-02 of which the preservation number is CGMCC No.20818. The complex microbial inoculant can solve the problem of fatty liver of pets by reducing indexes of total bilirubin, glutamic-pyruvic transaminase, glutamyltranspeptidase and alkaline phosphatase of the pets and reducing the volume of liver fat of the pets, promotes appetite and mental recovery of the pets, and is safe to use, free of peculiar smell and free of toxic and side effects. The growth and development of pets are facilitated.

Description

technical field [0001] The invention belongs to the technical field of pet breeding, and in particular relates to the application of a compound bacterial agent containing bile acid in the preparation of a preparation for preventing and treating fatty liver in pets. [0002] technical background [0003] Fatty liver is a relatively common liver disease in pets, which is often caused by hunger strike after stress. When the glycogen in the pet's body is exhausted, the body starts to use fat for energy, but the liver of the pet is not good at using fat by nature. When a large amount of fat pours into the liver, it is easy to cause fat deposition in the liver, thus forming fatty liver . The sick pets will further go on hunger strike at this time, and begin to vomit, depressed, and even convulsions of hepatic encephalopathy in severe cases. If the pet is not intervened in time, it will cause the death of the pet. In addition, the common triplet inflammation in pets: hepatitis, en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/61A61K35/741A61K31/575A61P1/16A61P1/14A61P25/00
CPCA61K36/61A61K35/741A61K31/575A61P1/16A61P1/14A61P25/00A61K2300/00
Inventor 李金宝曹爱智娄倩倩
Owner SHANDONG LONGCHANG ANIMAL HEALTH PROD
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