Heavy metal resistant pestalotiopsis neohirsuta microorganism and application thereof
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A technology of Polychaeta pseudodiscoides and heavy metals, applied in the direction of microorganisms, microorganism-based methods, applications, etc., can solve the problems of re-release of heavy metals, polluted soil, limited microbial biomass, etc.
Active Publication Date: 2021-06-25
HUNAN UNIV OF SCI & TECH
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Problems solved by technology
However, microbial control and remediation of heavy metal-contaminated soil and water also have disadvantages
For example, the biomass of microorganisms is limi
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Embodiment 1
[0030] (1) Take 1g of soil from the heavy metal polluted farmland around Xiangtan Manganese Mine;
[0031] (2) Use 1 mL of sterile water to dissolve the soil sample, react in a shaker at 150 rpm for 1 min, and let stand for 10 min;
[0032] (3) Take 1 mL of the supernatant, and serially dilute 10 1 、10 2 、10 3 、10 4 、10 5 times;
[0033] (4) Use PDA medium and Bengal red medium to culture microorganisms for 1-7 days, and the medium contains heavy metals of different concentrations, among which the Cr concentration is 100mg / L, 150mg / L, 200mg / L, and the Cd concentration is 100mg / L, 150mg / L. L, 200mg / L, Pb concentration 300mg / L, 400mg / L, 500mg / L, Mn concentration 5000mg / L, 7000mg / L, 10000mg / L;
[0034] (5) Screen heavy metal-resistant microorganisms for strain identification;
[0038] (1) Neopestalotiopsis sp. MCT-13 containing 50mg / L Cd 2+ PDA medium for 3 days;
[0039] (2) Add straw and continue to cultivate for 3 days, the concentration of straw is 100:1 (g:L), and the mixture of bacteria and straw is dried by circulating suction;
[0040] (3) 500 o Under the condition of C, burn for 2 hours in an oxygen barrier to prepare biochar;
[0041] (4) XRD characterization of biochar confirms Cd 2+ Convert to CdCO 3 precipitation, is effectively cured ( figure 2 ).
Embodiment 3
[0043] (1) Neopestalotiopsis sp. MCT-13 containing 200mg / L Ca 2+ PDA medium for 4 days;
[0044] (2) Add 100 mesh straw, the concentration of straw is 50:1 (g:L), and continue to cultivate for 10 days;
[0045] (3) at 121 o Under the condition of C, after steam reaction for 1 hour, freeze-drying;
[0046] (4) 600 o Under the condition of C, the biochar composite material was prepared by firing for 1 hour in an oxygen barrier;
[0047] (5) XRD characterization confirmed Ca 2+ CaCO 3 , successfully prepared loaded CaCO 3 The biochar composite material ( image 3 ).
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Abstract
The invention relates to a heavy metal resistant pestalotiopsis neohirsuta microorganism MCT-13, which is a pestalotiopsis sp. MCT-13 (Neopestalotiopsis sp.MCT-13) and preserved in the China Center for Type Culture Collection and has the preservation number of CCTCC NO M 2020543. The Neopestalotiopsis sp.MCT-13 disclosed by the invention is easy to culture, large in biomass and environment-friendly, and has composite heavy metal resistance and adsorption capacity. Under the condition of heavy metal ions Cr < 6 + >, Cd < 2 + > and Pb < 2 + >, after Neopestalotiopsis sp.MCT-13 and plant straw are co-cultured, a culture product is collected and pyrolyzed to prepare the biochar, and the curing of the heavy metal ions Cr < 6 + >, Cd < 2 + > and Pb < 2 + > can be effectively realized. Neopestalotiopsis sp.MCT-13 and plant straws under the conditions of nitrogen, phosphorus and metal ions Fe < 3 + >, Fe < 2 + >, Mn < 2 + >, Mg < 2 + > and Ca < 2 + >, collecting a culture product, and preparing the biochar composite material by combining a hydrothermal method with a high-temperature pyrolysis method, and the biochar composite material can be applied to heavy metal pollution remediation of water and soil.
Description
technical field [0001] The invention relates to the preparation of biochar by combining heavy metal-resistant microorganisms with straws to control and restore heavy metal pollution in water bodies and soils. Background technique [0002] With the rapid development of social economy, people have higher and higher requirements for environmental quality. Heavy metal pollutants pose a serious threat to water bodies and soil, and damage human health. At present, the methods for controlling and remediating heavy metal pollution in soil and water mainly include physical, chemical and biological methods. Biological control and remediation of heavy metal-contaminated soil and water mainly include phytoremediation and microbial remediation. Phytoremediation is a method of using plants to control and repair heavy metal pollution. Due to the long plant growth cycle, the plant grows slowly after entering winter, and even withers and dies. The phytoremediation cycle is long and the ef...
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