Identification method and application of M1 type macrophages
A macrophage and identification method technology, applied in the field of cell detection and typing, can solve the problems of insufficient index specificity, time-consuming, difficult clinical transformation, etc., and achieve the effect of high sensitivity and specificity
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Embodiment 1
[0037] This example is used to illustrate the typing and identification of macrophages, especially M1 type macrophages, by the identification method of the present invention.
[0038] 1. Induction, culture and collection of M0, M1 and M2 macrophages
[0039] Day0: (1) Take two six-well plates and prepare to culture 6-well cells, with two wells each for M0, M1, and M2. Add 1mL of cell culture solution to each well; (2) Take the cells in the culture flask that have been cultured for three days and prepare to subculture, and the cell concentration is about 10 6 cells / mL, add 1 mL of cell suspension to each well; (3) take pictures in a biological safety cabinet (20x, phase contrast mode); (4) add 2 μL of 100 μg / mL PMA to each well (final concentration 100 ng / mL); Induction for 2 days.
[0040] Day2: (1) Take pictures in the biological safety cabinet (20x, phase contrast mode); (2) Replace each well with 2 mL of culture medium; (3) M1 2 wells + 2 μL 100 μg / mL LPS (final concentra...
Embodiment 2
[0050] This example is used to illustrate the typing and identification of macrophages, especially M1 type macrophages, by the identification method of the present invention.
[0051] 1. Induction of M0, M1, and M2 macrophage cells, and intervention with inhibitors of M1 macrophages and agonists of M1 macrophages, cultured and collected.
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